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Mouse Anti-PDCD6 Recombinant Antibody (2B4) (CBMAB-P1164-YC)

Provided herein is a Mouse monoclonal antibody against Human Programmed Cell Death 6. The antibody can be used for immunoassay techniques, such as WB, ELISA, IF.
See all PDCD6 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2B4
Antibody Isotype
IgG2a, κ
Application
WB, ELISA, IF

Basic Information

Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Programmed Cell Death 6
Introduction
PDCD6 is a calcium-binding protein belonging to the penta-EF-hand protein family. Calcium binding is important for homodimerization and for conformational changes required for binding to other protein partners. This gene product participates in T cell receptor-, Fas-, and glucocorticoid-induced programmed cell death. In mice deficient for this gene product, however, apoptosis was not blocked suggesting this gene product is functionally redundant. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is also located on the short arm of chromosome 5.
Entrez Gene ID
10016
UniProt ID
O75340
Alternative Names
Programmed Cell Death 6; Apoptosis-Linked Gene 2 Protein Homolog; ALG-2; ALG2; Probable Calcium-Binding Protein ALG-2; Apoptosis-Linked Gene 2 Protein; Programmed Cell Death Protein 6; Apoptosis-Linked Gene-2; PEF1B;
Function
Calcium sensor that plays a key role in processes such as endoplasmic reticulum (ER)-Golgi vesicular transport, endosomal biogenesis or membrane repair. Acts as an adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes in response to calcium: calcium-binding triggers exposure of apolar surface, promoting interaction with different sets of proteins thanks to 3 different hydrophobic pockets, leading to translocation to membranes (PubMed:20691033, PubMed:25667979).
Involved in ER-Golgi transport by promoting the association between PDCD6IP and TSG101, thereby bridging together the ESCRT-III and ESCRT-I complexes (PubMed:19520058).
Together with PEF1, acts as calcium-dependent adapter for the BCR(KLHL12) complex, a complex involved in ER-Golgi transport by regulating the size of COPII coats (PubMed:27716508).
In response to cytosolic calcium increase, the heterodimer formed with PEF1 interacts with, and bridges together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification (PubMed:27716508).
Involved in the regulation of the distribution and function of MCOLN1 in the endosomal pathway (PubMed:19864416).
Promotes localization and polymerization of TFG at endoplasmic reticulum exit site (PubMed:27813252).
Required for T-cell receptor-, Fas-, and glucocorticoid-induced apoptosis (By similarity).
May mediate Ca2+-regulated signals along the death pathway: interaction with DAPK1 can accelerate apoptotic cell death by increasing caspase-3 activity (PubMed:16132846).
Its role in apoptosis may however be indirect, as suggested by knockout experiments (By similarity).
May inhibit KDR/VEGFR2-dependent angiogenesis; the function involves inhibition of VEGF-induced phosphorylation of the Akt signaling pathway (PubMed:21893193).
In case of infection by HIV-1 virus, indirectly inhibits HIV-1 production by affecting viral Gag expression and distribution (PubMed:27784779).
Isoform 2
Has a lower Ca2+ affinity than isoform 1 (By similarity).
Biological Process
Activation of cysteine-type endopeptidase activity involved in apoptotic processIEA:Ensembl
AngiogenesisIEA:UniProtKB-KW
Apoptotic signaling pathwayManual Assertion Based On ExperimentTAS:ProtInc
Cellular response to heatManual Assertion Based On ExperimentIDA:UniProtKB
COPII vesicle coatingManual Assertion Based On ExperimentIMP:UniProtKB
Endoplasmic reticulum to Golgi vesicle-mediated transportManual Assertion Based On ExperimentIMP:UniProtKB
Intracellular protein transportManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of protein kinase B signalingManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of TOR signalingManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of vascular endothelial growth factor receptor signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Neural crest cell developmentManual Assertion Based On ExperimentIMP:UniProtKB
Neural crest formationManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of angiogenesisManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of cysteine-type endopeptidase activity involved in apoptotic processManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of endothelial cell migrationManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of endothelial cell proliferationManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of protein monoubiquitinationManual Assertion Based On ExperimentIMP:UniProtKB
Response to calcium ionISS:UniProtKB
Vascular endothelial growth factor receptor-2 signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Endoplasmic reticulum membrane
Cytoplasmic vesicle, COPII-coated vesicle membrane
Cytoplasm
Nucleus
Endosome
Interaction with RBM22 induces relocalization from the cytoplasm to the nucleus (PubMed:17045351).
Translocated from the cytoplasm to the nucleus after heat shock cell treatment. Accumulates in cytoplasmic vesicle-like organelles after heat shock treatment, which may represent stress granules (PubMed:21122810).
In response to calcium increase, relocates from cytoplasm to COPII vesicle coat (PubMed:27716508).
Localizes to endoplasmic reticulum exit site (ERES) (PubMed:27813252).
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For research use only. Not intended for any clinical use.

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