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Rabbit Anti-Phospho-IRS1 (Ser318) Recombinant Antibody (D51C3) (CBMAB-CP1223-LY)

The product is antibody recognizes Phospho-IRS1 (Ser318). The antibody D51C3 immunoassay techniques such as: WB,IP.
See all Phospho-IRS1 (Ser318) antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
D51C3
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Immunogen
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser318 of mouse IRS-1 protein.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Insulin Receptor Substrate 1
Introduction
This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009]
Entrez Gene ID
Human3667
Mouse16367
Rat25467
UniProt ID
HumanP35568
MouseP35569
RatP35570
Alternative Names
Insulin Receptor Substrate 1
Function
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).
Biological Process
Cellular response to fatty acidISS:ARUK-UCL
Cellular response to insulin stimulusManual Assertion Based On ExperimentIMP:BHF-UCL
Glucose homeostasisManual Assertion Based On ExperimentTAS:BHF-UCL
Insulin receptor signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Insulin-like growth factor receptor signaling pathwayManual Assertion Based On ExperimentIPI:UniProtKB
Negative regulation of insulin receptor signaling pathwayISS:BHF-UCL
Negative regulation of insulin secretionManual Assertion Based On ExperimentIDA:BHF-UCL
Phosphatidylinositol 3-kinase signalingManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of cell population proliferation1 PublicationNAS:BHF-UCL
Positive regulation of fatty acid beta-oxidationManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of glucose importManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of glucose metabolic processManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of glycogen biosynthetic processManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of insulin receptor signaling pathwayManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of phosphatidylinositol 3-kinase activityISS:BHF-UCL
Response to insulinManual Assertion Based On ExperimentIDA:BHF-UCL
Response to peptide hormoneISS:BHF-UCL
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
Caveola; Cytoplasm; Cytosol; Insulin receptor complex; Intracellular membrane-bounded organelle; Nucleoplasm; Nucleus; Plasma membrane
Involvement in disease
Diabetes mellitus, non-insulin-dependent (NIDDM):
A multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension and hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
PTM
Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor (By similarity).
Phosphorylation of Tyr-896 is required for GRB2-binding (By similarity).
Phosphorylated by ALK. Phosphorylated at Ser-270, Ser-307, Ser-636 and Ser-1101 by RPS6KB1; phosphorylation induces accelerated degradation of IRS1 (PubMed:18952604).
Phosphorylated on tyrosine residues in response to insulin (PubMed:23401856).
In skeletal muscles, dephosphorylated on Tyr-612 by TNS2 under anabolic conditions; dephosphorylation results in the proteasomal degradation of IRS1 (PubMed:23401856).
Ubiquitinated by the Cul7-RING(FBXW8) complex in a mTOR-dependent manner, leading to its degradation: the Cul7-RING(FBXW8) complex recognizes and binds IRS1 previously phosphorylated by S6 kinase (RPS6KB1 or RPS6KB2).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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