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Mouse Anti-PKN1 Recombinant Antibody (1A4) (CBMAB-P1949-YC)

Provided herein is a Mouse monoclonal antibody against Human Protein Kinase N1. The antibody can be used for immunoassay techniques, such as ELISA, IHC-P, WB.
See all PKN1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1A4
Antibody Isotype
IgG2a, κ
Application
ELISA, IHC-P, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
PKN1
Introduction
PKN1 (Protein Kinase N1) is a protein coding gene. Diseases associated with PKN1 include Paraneoplastic Cerebellar Degeneration. Among its related pathways are Signaling by Rho GTPases and RhoA signaling pathway. Gene Ontology annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity.
Entrez Gene ID
5585
UniProt ID
Q16512
Alternative Names
PAK2; PRK2; PRKCL2; PRO2042; Pak-2; STK7
Function
PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (AR)-dependent transcription, by being recruited to AR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser-159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.
Biological Process
B cell apoptotic processIEA:Ensembl
B cell homeostasisIEA:Ensembl
Chromatin organizationIEA:UniProtKB-KW
Epithelial cell migrationManual Assertion Based On ExperimentIMP:UniProtKB
Histone H3-T11 phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Hyperosmotic responseIEA:Ensembl
Intracellular signal transductionManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of B cell proliferationIEA:Ensembl
Negative regulation of protein kinase activityIEA:Ensembl
Peptidyl-serine phosphorylationManual Assertion Based On ExperimentIBA:GO_Central
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of androgen receptor signaling pathwayTAS:Reactome
Regulation of cell motilityManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of germinal center formationIEA:Ensembl
Regulation of immunoglobulin productionIEA:Ensembl
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
Renal system processIEA:Ensembl
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Spleen developmentIEA:Ensembl
Cellular Location
Cytoplasm
Nucleus
Endosome
Cell membrane
Cleavage furrow
Midbody
Associates with chromatin in a ligand-dependent manner. Localization to endosomes is mediated via its interaction with RHOB. Association to the cell membrane is dependent on Ser-377 phosphorylation. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis.
PTM
Autophosphorylated; preferably on serine. Phosphorylated during mitosis.
Activated by limited proteolysis with trypsin.
(Microbial infection) In case of infection, polyubiquitinated by the bacterial E3 ubiquitin-protein ligase SspH1, leading to its proteasomal degradation.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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