Mouse Anti-PRK2 Recombinant Antibody (CBT3737) (V2LY-0625-LY2677)

Name: Mouse Anti-PRK2 Recombinant Antibody (CBT3737)
SKU: V2LY-0625-LY2677
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-PRK2 Recombinant Antibody (CBT3737) (V2LY-0625-LY2677) in WB

Mouse Anti-PRK2 Recombinant Antibody (CBT3737) (V2LY-0625-LY2677) in IHC

Mouse Anti-PRK2 Recombinant Antibody (CBT3737) (V2LY-0625-LY2677) in FC

Mouse Anti-PRK2 Recombinant Antibody (CBT3737) (V2LY-0625-LY2677) in ELISA

Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBT3737

Application

WB, IHC, FC

Immunogen

Purified recombinant fragment of human PRK2 expressed in E. Coli.

Host Species

Mouse

Specificity

Human, Monkey, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC-P	1:200-1:1,000
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Preservative

Sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

PRK2

Entrez Gene ID

5586

UniProt ID

Q16513

Function

PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c-fos serum response factor (SRF). Involved in the negative regulation of ciliogenesis (PubMed:27104747).
(Microbial infection) Phosphorylates HCV NS5B leading to stimulation of HCV RNA replication.

Biological Process

Apical junction assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Apoptotic processIEA:UniProtKB-KW
Cell adhesionIEA:UniProtKB-KW
Cell cycleIEA:UniProtKB-KW
Cell divisionIEA:UniProtKB-KW
Cell projection organizationIEA:UniProtKB-KW
Epithelial cell migrationManual Assertion Based On ExperimentIDA:UniProtKB
Intracellular signal transductionManual Assertion Based On ExperimentIBA:GO_Central
Peptidyl-serine phosphorylationManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of cytokinesisManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of mitotic cell cycleManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of viral genome replicationManual Assertion Based On ExperimentIMP:AgBase
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of cell motilityManual Assertion Based On ExperimentIMP:UniProtKB
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Cytoplasm
Nucleus
Membrane
Cell projection, lamellipodium
Cytoplasm, cytoskeleton
Cleavage furrow
Midbody
Cell junction
Colocalizes with PTPN13 in lamellipodia-like structures, regions of large actin turnover. Accumulates during telophase at the cleavage furrow and concentrates finally around the midbody in cytokinesis. Recruited to nascent cell-cell contacts at the apical surface of cells. In the course of viral infection, colocalizes with HCV NS5B at perinuclear region in the cytoplasm.

PTM

Autophosphorylated. Phosphorylated during mitosis. Phosphorylated by CDK10 (PubMed:27104747).
Activated by limited proteolysis with trypsin (By similarity).
Proteolytically cleaved by caspase-3 during the induction of apoptotic cell death.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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