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Mouse Anti-PRKAG3 Recombinant Antibody (CBT3997) (V2LY-0625-LY617)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBT3997
Antibody Isotype
IgG1
Application
IHC, ICC

Basic Information

Immunogen
Purified recombinant fragment of human PRKAG3 (AA: 9-151) expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
IHC-P1:200-1:1,000
ICC1:200-1:1,000
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
protein kinase AMP-activated non-catalytic subunit gamma 3
Entrez Gene ID
53632
UniProt ID
Q9UGI9
Function
AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. AMPK also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. The AMPK gamma3 subunit is a non-catalytic subunit with a regulatory role in muscle energy metabolism (PubMed:17878938).
It mediates binding to AMP, ADP and ATP, leading to AMPK activation or inhibition: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.
Biological Process
Cellular response to glucose starvationManual Assertion Based On ExperimentIBA:GO_Central
Cellular response to nutrient levelsManual Assertion Based On ExperimentIDA:ComplexPortal
Fatty acid biosynthetic processIEA:UniProtKB-KW
Glycogen biosynthetic processIEA:Ensembl
Glycolytic processIEA:Ensembl
Intracellular signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Protein phosphorylationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of catalytic activityManual Assertion Based On ExperimentIBA:GO_Central
Regulation of protein serine/threonine kinase activityIEA:InterPro
Response to muscle activity involved in regulation of muscle adaptationIEA:Ensembl
Cellular Location
cytoplasm
cytosol
extracellular space
nucleoplasm
nucleotide-activated protein kinase complex
nucleus
PTM
Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK.
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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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