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Mouse Anti-RAG2 Recombinant Antibody (CBT1090) (V2LY-0625-LY3043)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBT1090
Antibody Isotype
IgG
Application
WB, ELISA

Basic Information

Immunogen
Purified recombinant fragment of human RAG-2 (350-527aa) expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
ELISA1:10,000
WB1:500-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Buffer
BSA, Glycerol
Preservative
Sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Recombination Activating 2
Entrez Gene ID
5897
UniProt ID
P55895
Function
Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3 (By similarity).
Biological Process
Biological Process B cell differentiationISS:UniProtKB
Biological Process B cell homeostatic proliferationIEA:Ensembl
Biological Process B cell lineage commitmentIEA:Ensembl
Biological Process chromatin organizationIEA:UniProtKB-KW
Biological Process defense response to bacteriumIEA:Ensembl
Biological Process DN2 thymocyte differentiationIEA:Ensembl
Biological Process mature B cell differentiation involved in immune responseIEA:Ensembl
Biological Process negative regulation of T cell differentiation in thymusIEA:Ensembl
Biological Process organ growthIEA:Ensembl
Biological Process positive regulation of organ growthIEA:Ensembl
Biological Process pre-B cell allelic exclusionISS:UniProtKB
Biological Process T cell differentiation in thymusISS:UniProtKB
Biological Process T cell lineage commitmentIEA:Ensembl
Biological Process V(D)J recombinationISS:UniProtKB
Cellular Location
Nucleus
Involvement in disease
Combined cellular and humoral immune defects with granulomas (CHIDG):
Immunodeficiency disease with granulomas in the skin, mucous membranes, and internal organs. Other characteristics include hypogammaglobulinemia, a diminished number of T and B-cells, and sparse thymic tissue on ultrasonography.
Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-positive (T(-)B(-)NK(+) SCID):
A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Omenn syndrome (OS):
Severe immunodeficiency characterized by the presence of activated, anergic, oligoclonal T-cells, hypereosinophilia, and high IgE levels.
Alpha/beta T-cell lymphopenia, with gamma/delta T-cell expansion, severe cytomegalovirus infection and autoimmunity (T-CMVA):
An immunological disorder characterized by oligoclonal expansion of TCR gamma/delta T-cells, TCR alpha/beta T-cell lymphopenia, severe, disseminated cytomegalovirus infection and autoimmune cytopenia.
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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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