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Mouse Anti-RFWD3 Recombinant Antibody (6B4) (CBMAB-R2378-CN)

This product is a Mouse antibody that recognizes RFWD3. The antibody 6B4 can be used for immunoassay techniques such as: ELISA, WB.
See all RFWD3 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
6B4
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Immunogen
Partial recombinant corresponding to aa. 651-750 from human RFWD3 (H59371) with GST tag
Specificity
Human
Antibody Isotype
IgG2a, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2

Target

Full Name
ring finger and WD repeat domain 3
Introduction
RFWD3 (Ring Finger And WD Repeat Domain 3) is a Protein Coding gene. Diseases associated with RFWD3 include Fanconi Anemia, Complementation Group W and Fanconi Anemia, Complementation Group A. Gene Ontology (GO) annotations related to this gene include ligase activity and p53 binding.
Entrez Gene ID
55159
UniProt ID
Q6PCD5
Alternative Names
Ring Finger And WD Repeat Domain 3; RING Finger And WD Repeat Domain-Containing Protein 3; RING Finger Protein 201; RNF201; RING-Type E3 Ubiquitin Transferase RFWD3; E3 Ubiquitin-Protein Ligase RFWD3; EC 2.3.2.27; FANCW;
Function
E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage (PubMed:21504906, PubMed:21558276, PubMed:26474068, PubMed:28575657, PubMed:28575658).
Plays a key role in RPA-mediated DNA damage signaling and repair (PubMed:21504906, PubMed:21558276, PubMed:26474068, PubMed:28575657, PubMed:28575658, PubMed:28691929).
Acts by mediating ubiquitination of the RPA complex (RPA1, RPA2 and RPA3 subunits) and RAD51 at stalled replication forks, leading to remove them from DNA damage sites and promote homologous recombination (PubMed:26474068, PubMed:28575657, PubMed:28575658).
Also mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint (PubMed:20173098).
May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome (PubMed:20173098).
In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53 (PubMed:20173098).
Biological Process
Biological Process cellular response to DNA damage stimulusManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process double-strand break repair via homologous recombinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process interstrand cross-link repairManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process mitotic G1 DNA damage checkpoint signalingManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process protein ubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of DNA damage checkpointManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process replication fork processingManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process response to ionizing radiationManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Nucleus
Nucleus, PML body
Cytoplasm
In undamaged cells, found both in the cytoplasm and in the nucleus, partially associated with PML nuclear bodies (PubMed:21558276).
In response to replication block, such as that caused by hydroxyurea treatment, or to DNA damage caused by ionizing radiations or doxorubicin, recruited to the nucleus, to stalled replication forks or to sites of DNA repair (PubMed:21504906, PubMed:28575657).
This recruitment depends upon RPA2 (PubMed:21504906).
Involvement in disease
Fanconi anemia, complementation group W (FANCW):
A form of Fanconi anemia, a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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