Mouse Anti-RHOA Recombinant Antibody (CBYC-A1035) (CBMAB-A4705-YC)

Mouse

Human, Mouse, Rat

CBYC-A1035

IgG

WB, ICC

Human, Mouse, Rat

IgG

Monoclonal

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Ras Homolog Family Member A

RHOA is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and r

Ras Homolog Family Member A; ARH12; RHO12; ARHA; Ras Homolog Gene Family, Member A; Aplysia Ras-Related Homolog 12; Small GTP Binding Protein RhoA;

Small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Mainly associated with cytoskeleton organization, in active state binds to a variety of effector proteins to regulate cellular responses such as cytoskeletal dynamics, cell migration and cell cycle. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers (PubMed:8910519, PubMed:9121475, PubMed:31570889).
Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis (PubMed:16236794, PubMed:12900402).
Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion (PubMed:20974804, PubMed:23940119).
Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly (PubMed:19934221).
The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization (PubMed:20937854).
Regulates KCNA2 potassium channel activity by reducing its location at the cell surface in response to CHRM1 activation; promotes KCNA2 endocytosis (PubMed:9635436, PubMed:19403695).
Acts as an allosteric activator of guanine nucleotide exchange factor ECT2 by binding in its activated GTP-bound form to the PH domain of ECT2 which stimulates the release of PH inhibition and promotes the binding of substrate RHOA to the ECT2 catalytic center (PubMed:31888991).
May be an activator of PLCE1 (PubMed:16103226).
In neurons, involved in the inhibition of the initial spine growth. Upon activation by CaMKII, modulates dendritic spine structural plasticity by relaying CaMKII transient activation to synapse-specific, long-term signaling (By similarity).
Acts as a regulator of platelet alpha-granule release during activation and aggregation of platelets (By similarity).
(Microbial infection) Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague.

Biological Process actin cytoskeleton organizationManual Assertion Based On ExperimentTAS:UniProtKB
Biological Process actin cytoskeleton reorganizationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process actin filament organizationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process alpha-beta T cell lineage commitmentIEA:Ensembl
Biological Process androgen receptor signaling pathwayIEA:Ensembl
Biological Process angiotensin-mediated vasoconstriction involved in regulation of systemic arterial blood pressureIEA:Ensembl
Biological Process apical junction assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process apolipoprotein A-I-mediated signaling pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process beta selectionIEA:Ensembl
Biological Process cell junction assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process cell migrationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process cell-matrix adhesionIEA:Ensembl
Biological Process cellular response to chemokineManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process cellular response to cytokine stimulusManual Assertion Based On ExperimentIMP:ARUK-UCL
Biological Process cellular response to lipopolysaccharideManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process cerebral cortex cell migrationIEA:Ensembl
Biological Process cleavage furrow formationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process cortical cytoskeleton organizationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process cytoplasmic microtubule organizationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process endothelial cell migrationManual Assertion Based On ExperimentIGI:MGI
Biological Process endothelial tube lumen extensionManual Assertion Based On ExperimentIGI:MGI
Biological Process establishment of epithelial cell apical/basal polarityManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process establishment or maintenance of cell polarityManual Assertion Based On ExperimentIBA:GO_Central
Biological Process forebrain radial glial cell differentiationIEA:Ensembl
Biological Process GTP metabolic processIEA:Ensembl
Biological Process kidney developmentIEA:Ensembl
Biological Process mitotic cleavage furrow formationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process mitotic spindle assemblyManual Assertion Based On ExperimentIMP:BHF-UCL
Biological Process motor neuron apoptotic processIEA:Ensembl
Biological Process negative chemotaxisManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process negative regulation of cell migration involved in sprouting angiogenesisManual Assertion Based On ExperimentIGI:MGI
Biological Process negative regulation of cell sizeManual Assertion Based On ExperimentIMP:CAFA
Biological Process negative regulation of cell-substrate adhesionISS:ARUK-UCL
Biological Process negative regulation of I-kappaB kinase/NF-kappaB signalingIEA:Ensembl
Biological Process negative regulation of intracellular steroid hormone receptor signaling pathwayIEA:Ensembl
Biological Process negative regulation of motor neuron apoptotic processIEA:Ensembl
Biological Process negative regulation of neuron differentiationIEA:Ensembl
Biological Process negative regulation of neuron projection developmentIEA:Ensembl
Biological Process negative regulation of oxidative phosphorylationIEA:Ensembl
Biological Process negative regulation of reactive oxygen species biosynthetic processIEA:Ensembl
Biological Process negative regulation of vascular associated smooth muscle cell migrationIEA:Ensembl
Biological Process negative regulation of vascular associated smooth muscle cell proliferationIEA:Ensembl
Biological Process neuron migrationIEA:Ensembl
Biological Process neuron projection morphogenesisIEA:Ensembl
Biological Process odontogenesisIEA:Ensembl
Biological Process ossification involved in bone maturationISS:BHF-UCL
Biological Process positive regulation of actin filament polymerizationIEA:Ensembl
Biological Process positive regulation of alpha-beta T cell differentiationIEA:Ensembl
Biological Process positive regulation of cell growthIEA:Ensembl
Biological Process positive regulation of cysteine-type endopeptidase activity involved in apoptotic processIEA:Ensembl
Biological Process positive regulation of cytokinesisManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process positive regulation of I-kappaB kinase/NF-kappaB signalingManual Assertion Based On ExperimentIEP:UniProtKB
Biological Process positive regulation of leukocyte adhesion to vascular endothelial cellManual Assertion Based On ExperimentIMP:ARUK-UCL
Biological Process positive regulation of lipase activityManual Assertion Based On ExperimentIDA:AgBase
Biological Process positive regulation of neuron apoptotic processIEA:Ensembl
Biological Process positive regulation of neuron differentiationManual Assertion Based On ExperimentIMP:MGI
Biological Process positive regulation of NIK/NF-kappaB signalingManual Assertion Based On ExperimentIMP:ARUK-UCL
Biological Process positive regulation of podosome assemblyIEA:Ensembl
Biological Process positive regulation of protein serine/threonine kinase activityManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of stress fiber assemblyManual Assertion Based On ExperimentIDA:MGI
Biological Process positive regulation of T cell migrationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process positive regulation of translationIEA:Ensembl
Biological Process positive regulation of vascular associated smooth muscle contractionIEA:Ensembl
Biological Process regulation of actin cytoskeleton organizationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of calcium ion transportIEA:Ensembl
Biological Process regulation of cell migrationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulation of cell shapeManual Assertion Based On ExperimentIBA:GO_Central
Biological Process regulation of dendrite developmentIEA:Ensembl
Biological Process regulation of focal adhesion assemblyManual Assertion Based On ExperimentTAS:ARUK-UCL
Biological Process regulation of microtubule cytoskeleton organizationIEA:Ensembl
Biological Process regulation of modification of postsynaptic actin cytoskeletonManual Assertion Based On ExperimentIMP:SynGO
Biological Process regulation of neural precursor cell proliferationIEA:Ensembl
Biological Process regulation of osteoblast proliferationISS:BHF-UCL
Biological Process regulation of systemic arterial blood pressure by endothelinIEA:Ensembl
Biological Process regulation of transcription by RNA polymerase IIIEA:Ensembl
Biological Process response to amino acidIEA:Ensembl
Biological Process response to ethanolIEA:Ensembl
Biological Process response to glucocorticoidIEA:Ensembl
Biological Process response to glucoseIEA:Ensembl
Biological Process response to hypoxiaIEA:Ensembl
Biological Process response to mechanical stimulusIEA:Ensembl
Biological Process response to xenobiotic stimulusIEA:Ensembl
Biological Process Rho protein signal transductionManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process Roundabout signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process skeletal muscle satellite cell migrationISS:AgBase
Biological Process skeletal muscle tissue developmentIEA:Ensembl
Biological Process stress fiber assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process stress-activated protein kinase signaling cascadeIEA:Ensembl
Biological Process substantia nigra developmentManual Assertion Based On ExperimentHEP:UniProtKB
Biological Process substrate adhesion-dependent cell spreadingManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process trabecula morphogenesisISS:BHF-UCL
Biological Process Wnt signaling pathway, planar cell polarity pathway1 PublicationNAS:ParkinsonsUK-UCL
Biological Process wound healing, spreading of cellsISS:AgBase

Cell membrane
Cytoplasm, cytoskeleton
Cleavage furrow
Cytoplasm, cell cortex
Midbody
Cell projection, lamellipodium
Cell projection, dendrite
Nucleus
Localized to cell-cell contacts in calcium-treated keratinocytes (By similarity).
Translocates to the equatorial region before furrow formation in a ECT2-dependent manner. Localizes to the equatorial cell cortex (at the site of the presumptive furrow) in early anaphase in an activated form and in a myosin- and actin-independent manner.

Ectodermal dysplasia with facial dysmorphism and acral, ocular, and brain anomalies (EDFAOB):
A neuroectodermal syndrome characterized by linear hypopigmentation, alopecia, apparently asymptomatic leukoencephalopathy, and facial, ocular, dental and acral anomalies. Patients show no intellectual or neurologic impairment.

(Microbial infection) Substrate for botulinum ADP-ribosyltransferase.
(Microbial infection) Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage.
(Microbial infection) AMPylation at Tyr-34 and Thr-37 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
(Microbial infection) Glycosylated at Tyr-34 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of Rho and leads to actin disassembly.
(Microbial infection) Glucosylated at Thr-37 by C.difficile toxins TcdA and TcdB in the colonic epithelium (PubMed:7777059, PubMed:7775453, PubMed:24905543).
Monoglucosylation completely prevents the recognition of the downstream effector, blocking the GTPases in their inactive form, leading to actin cytoskeleton disruption and cell death, resulting in the loss of colonic epithelial barrier function (PubMed:7777059, PubMed:7775453).
(Microbial infection) Glycosylated (O-GlcNAcylated) at Thr-37 by C.novyi toxin TcdA (PubMed:8810274).
O-GlcNAcylation completely prevents the recognition of the downstream effector, blocking the GTPases in their inactive form, leading to actin cytoskeleton disruption (PubMed:8810274).
(Microbial infection) Stearoylated By S.flexneri N-epsilon-fatty acyltransferase IcsB, thereby disrupting the host actin cytoskeleton.
Phosphorylation by PRKG1 at Ser-188 inactivates RHOA signaling (PubMed:11162591).
Phosphorylation by SLK at Ser-188 in response to AGTR2 activation (By similarity).
Ubiquitinated by the BCR(KCTD13) and BCR(TNFAIP1) E3 ubiquitin ligase complexes, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and synaptic transmission in neurons.
Serotonylation of Gln-63 by TGM2 during activation and aggregation of platelets leads to constitutive activation of GTPase activity.