Mouse Anti-RTN4 Recombinant Antibody (M364) (CBMAB-N4226-WJ)

Mouse

Human, Rat, Mouse

M364

IgG1

WB, ELISA

Human, Rat, Mouse

IgG1

Monoclonal

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

reticulon 4

This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Reticulon 4; Neuroendocrine-Specific Protein C Homolog; Neurite Outgrowth Inhibitor; Foocen; RTN-X; NOGO; NSP; Neuroendocrine-Specific Protein; Neurite Growth Inhibitor 220; My043 Protein; Nogo Protein; Human NogoA; Reticulon 5;

Required to induce the formation and stabilization of endoplasmic reticulum (ER) tubules (PubMed:27619977, PubMed:25612671, PubMed:24262037).
They regulate membrane morphogenesis in the ER by promoting tubular ER production (PubMed:27619977, PubMed:25612671, PubMed:24262037, PubMed:27786289).
They influence nuclear envelope expansion, nuclear pore complex formation and proper localization of inner nuclear membrane proteins (PubMed:26906412).
However each isoform have specific functions mainly depending on their tissue expression specificities (Probable).
Isoform A
Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS (PubMed:10667797, PubMed:11201742).
Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex (By similarity).
Acts as a negative regulator of central nervous system angiogenesis. Inhibits spreading, migration and sprouting of primary brain microvascular endothelial cells (MVECs). Also induces the retraction of MVECs lamellipodia and filopodia in a ROCK pathway-dependent manner (By similarity).
Isoform B
Mainly function in endothelial cells and vascular smooth muscle cells, is also involved in immune system regulation (Probable). Modulator of vascular remodeling, promotes the migration of endothelial cells but inhibits the migration of vascular smooth muscle cells. Regulates endothelial sphingolipid biosynthesis with direct effects on vascular function and blood pressure. Inhibits serine palmitoyltransferase, SPTLC1, the rate-limiting enzyme of the novo sphingolipid biosynthetic pathway, thereby controlling production of endothelial sphingosine-1-phosphate (S1P). Required to promote macrophage homing and functions such as cytokine/chemokine gene expression involved in angiogenesis, arteriogenesis and tissue repair. Mediates ICAM1 induced transendothelial migration of leukocytes such as monocytes and neutrophils and acute inflammation. Necessary for immune responses triggered by nucleic acid sensing TLRs, such as TLR9, is required for proper TLR9 location to endolysosomes. Also involved in immune response to LPS. Plays a role in liver regeneration through the modulation of hepatocytes proliferation (By similarity).
Reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration (PubMed:11126360).
With isoform C, inhibits BACE1 activity and amyloid precursor protein processing (PubMed:16965550).
Isoform C
Regulates cardiomyocyte apoptosis upon hypoxic conditions (By similarity).
With isoform B, inhibits BACE1 activity and amyloid precursor protein processing (PubMed:16965550).

Biological Process apoptotic process1 PublicationNAS:UniProtKB
Biological Process axonal fasciculationISS:UniProtKB
Biological Process blastocyst formationIEA:Ensembl
Biological Process brain developmentManual Assertion Based On ExperimentIBA:GO_Central
Biological Process cardiac epithelial to mesenchymal transitionIEA:Ensembl
Biological Process cell adhesion involved in sprouting angiogenesisIDA:UniProtKB
Biological Process cell migration involved in vasculogenesisISS:UniProtKB
Biological Process cellular response to hypoxiaISS:UniProtKB
Biological Process cellular sphingolipid homeostasisIEA:Ensembl
Biological Process central nervous system vasculogenesisISS:UniProtKB
Biological Process cerebral cortex radial glia-guided migrationISS:UniProtKB
Biological Process endoplasmic reticulum organizationIDA:UniProtKB
Biological Process endoplasmic reticulum tubular network formationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process endoplasmic reticulum tubular network membrane organizationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process endoplasmic reticulum tubular network organizationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process leukocyte migration involved in inflammatory responseISS:UniProtKB
Biological Process negative regulation of amyloid-beta formationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of axon extensionManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of cell growthManual Assertion Based On ExperimentIMP:DFLAT
Biological Process negative regulation of vasculogenesisISS:UniProtKB
Biological Process neuron differentiationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process nuclear pore complex assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process positive regulation of angiogenesisISS:UniProtKB
Biological Process positive regulation of artery morphogenesisISS:UniProtKB
Biological Process positive regulation of epithelial cell migrationManual Assertion Based On ExperimentIMP:CAFA
Biological Process positive regulation of ERBB3 signaling pathwayManual Assertion Based On ExperimentIMP:CAFA
Biological Process positive regulation of hepatocyte proliferationISS:UniProtKB
Biological Process positive regulation of macrophage cytokine productionISS:UniProtKB
Biological Process positive regulation of macrophage migrationISS:UniProtKB
Biological Process positive regulation of mammary gland epithelial cell proliferationManual Assertion Based On ExperimentIMP:CAFA
Biological Process positive regulation of neutrophil migrationISS:UniProtKB
Biological Process positive regulation of protein kinase B signalingManual Assertion Based On ExperimentIMP:CAFA
Biological Process positive regulation of protein localization to endoplasmic reticulumManual Assertion Based On ExperimentIDA:CAFA
Biological Process positive regulation of Rac protein signal transductionISS:UniProtKB
Biological Process positive regulation of toll-like receptor 9 signaling pathwayISS:UniProtKB
Biological Process protein localization to lysosomeISS:UniProtKB
Biological Process protein stabilizationManual Assertion Based On ExperimentIMP:CAFA
Biological Process regulation of apoptotic process1 PublicationNAS:UniProtKB
Biological Process regulation of branching morphogenesis of a nerveISS:UniProtKB
Biological Process regulation of cell migrationIDA:MGI

Isoform A
Endoplasmic reticulum membrane
Cell membrane
Anchored to the membrane of the endoplasmic reticulum (ER) through 2 putative transmembrane domains. Localizes throughout the ER tubular network (PubMed:27619977).
Co-localizes with TMEM33 at the ER sheets.
Isoform B
Endoplasmic reticulum membrane
Cell membrane
Cell junction
Mainly located on endoplasmic reticulum tubules and sheet edges (PubMed:27786289).
Upon ICAM1 engagement, redistributed toward endothelial junctions where interacts with CDH5 (PubMed:21183689).
Isoform C
Endoplasmic reticulum membrane

Cytoplasmic: 1-1018
Helical: 1019-1039
Lumenal: 1040-1133
Helical: 1134-1154
Cytoplasmic: 1155-1192