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Mouse Anti-SRSF1 Recombinant Antibody (CBXS-3104) (CBMAB-S5849-CQ)

This product is a mouse antibody that recognizes SRSF1. The antibody CBXS-3104 can be used for immunoassay techniques such as: IF, WB.
See all SRSF1 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBXS-3104
Antibody Isotype
IgG1
Application
IF, WB

Basic Information

Immunogen
Recombinant SF2/ASF protein
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
0.09% sodium azide
Concentration
0.5 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Serine And Arginine Rich Splicing Factor 1
Introduction
This gene encodes a member of the arginine/serine-rich splicing factor protein family. The encoded protein can either activate or repress splicing, depending on its phosphorylation state and its interaction partners. Multiple transcript variants have been found for this gene. There is a pseudogene of this gene on chromosome 13.
Entrez Gene ID
Human6426
Mouse110809
Rat689890
UniProt ID
HumanQ07955
MouseQ6PDM2
RatA0A8I6GMR8
Alternative Names
ASF; SF2; SF2p33; SFRS1; SRp30a
Function
Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway.
Biological Process
Biological Process alternative mRNA splicing, via spliceosomeManual Assertion Based On ExperimentIDA:GO_Central
Biological Process mRNA 5'-splice site recognitionManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process mRNA processingManual Assertion Based On ExperimentTAS:ProtInc
Biological Process mRNA splice site selectionManual Assertion Based On ExperimentTAS:ProtInc
Biological Process mRNA splicing, via spliceosomeManual Assertion Based On ExperimentIBA:GO_Central
Biological Process mRNA transportIEA:UniProtKB-KW
Biological Process regulation of RNA splicingISS:UniProtKB
Cellular Location
Cytoplasm
Nucleus speckle
In nuclear speckles. Shuttles between the nucleus and the cytoplasm (PubMed:12215544, PubMed:20308322, PubMed:9420331, PubMed:24449914).
Nuclear import is mediated via interaction with TNPO3 (PubMed:24449914).
PTM
Phosphorylated by CLK1, CLK2, CLK3 and CLK4. Phosphorylated by SRPK1 at multiple serines in its RS domain via a directional (C-terminal to N-terminal) and a dual-track mechanism incorporating both processive phosphorylation (in which the kinase stays attached to the substrate after each round of phosphorylation) and distributive phosphorylation steps (in which the kinase and substrate dissociate after each phosphorylation event). The RS domain of SRSF1 binds to a docking groove in the large lobe of the kinase domain of SRPK1 and this induces certain structural changes in SRPK1 and/or RRM 2 domain of SRSF1, allowing RRM 2 to bind the kinase and initiate phosphorylation. The cycles continue for several phosphorylation steps in a processive manner (steps 1-8) until the last few phosphorylation steps (approximately steps 9-12). During that time, a mechanical stress induces the unfolding of the beta-4 motif in RRM 2, which then docks at the docking groove of SRPK1. This also signals RRM 2 to begin to dissociate, which facilitates SRSF1 dissociation after phosphorylation is completed.
Asymmetrically dimethylated at arginines, probably by PRMT1, methylation promotes localization to nuclear speckles.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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