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Mouse Anti-TUBA8 Recombinant Antibody (CBYJT-5249) (CBMAB-T4834-YJ)


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Summary

Host Animal
Mouse
Specificity
Human, Dog, Monkey, Rat
Clone
CBYJT-5249
Antibody Isotype
IgG2a
Application
FC, ICC, IF, IHC, IHC-P, WB

Basic Information

Immunogen
Full length human recombinant protein of human TUBA8(NP_061816) produced in HEK293T cell
Specificity
Human, Dog, Monkey, Rat
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.3, 1% BSA, 50% Glycerol
Preservative
0.02% Sodium Azide
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Introduction
TUBA8 is a member of the alpha tubulin protein family. Alpha tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Mutations in TUBA8 are associated with polymicrogyria and optic nerve hypoplasia.
Entrez Gene ID
Human51807
Dog486762
Rat500377
Monkey710378
UniProt ID
HumanQ9NY65
DogA0A8C0PIX2
RatQ6AY56
MonkeyF6S2F3
Alternative Names
Tubulin Alpha 8; Tubulin Alpha Chain-Like 2; TUBAL2; Tubulin Alpha-8 Chain; Alpha-Tubulin 8; CDCBM8
Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Biological Process
Biological Process microtubule cytoskeleton organization Source:GO_Central1 Publication
Biological Process mitotic cell cycle Source:GO_Central1 Publication
Cellular Location
Cytoplasm, cytoskeleton
Involvement in disease
Cortical dysplasia, complex, with other brain malformations 8 (CDCBM8):
A disease characterized by extensive polymicrogyria, optic nerve hypoplasia, severe developmental delay, hypotonia, seizures, a dysplastic or absent corpus callosum and colpocephaly. Polymicrogyria is a malformation of the cortex in which the brain surface is irregular and characterized by an excessive number of small gyri with abnormal lamination. Polymicrogyria is a heterogeneous disorder, considered to be the result of postmigratory abnormal cortical organization.
PTM
Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866).
Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
Glutamylation is also involved in cilia motility (By similarity).
Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into cilia and flagella axonemes, which is required for their stability and maintenance. Flagella glycylation controls sperm motility. Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally.
The C-terminal phenylalanine residue is cleaved by KIAA0895L/MATCAP.
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For research use only. Not intended for any clinical use.

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