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Human Autoimmune Disease IgG Autoantibody Array (Glass Slide, 33 Targets) (Y0216) (AbAr-0216-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The Autoimmune Disease IgG Autoantibody Array contains 33 human proteins and double strand DNA(dsDNA) for simultaneous detection of human IgG autoantibodies presented in blood serum samples of common 10 autoimmune diseases, such as systemic lupus erythematosus (SLE), Sjøgren's syndrome, Rheumatoid arthritis, primary biliary cirrhosis, etc.
Specifications
Target Array
Autoimmune Disease IgG Autoantibody Array
Target
Alanyl-tRNA Synthetase, Gliadin, Cytokeratin 19 (KRT19), M/Scl 100, Calcitonin, DNA topoisomerase 1 (DNA topoisomerase I), Thrombopoietin (TPO), Cytokeratin 8 (KRT8), Glycine-tRNA ligase (AP-4-A synthetase/GlyRS), Histidine-tRNA ligase (HisRS), M2, PCNA, Sm core protein, Transglutaminase 2 (TGM2), Centromere protein B (CENP-B), Nuclear pore protein gp210 (POM210/Nucleoporin Nup210), Lupus La protein (SS-B), Mi-2, Myeloblastin (PR3/C-ANCA), Sp-100, snRNP70, Centromere protein A (CENP-A), Histone H2A, LC-1, Myeloperoxidase, TRIM21 (Ro(SS-A)/SS-A), Threonine-tRNA ligase (ThrRS), dsDNA, Histone H2B, Cytokeratin 20 (KRT20), Nuclear pore glycoprotein p62 (Nucleoporin Nup62), TRIM21 (Ro(SS-A)/SS-A), Thyroglobulin
Species Reactivity
Human
Sample Type
Serum
Applications
Application
Multiplex Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Glass Slide
Detection Assay
Sandwich-based
Protocol Outline
Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis
Components
Antibody Array Slide
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.
Background
Introduction
An autoimmune disease is a condition arising from an abnormal immune response to a normal body part. There are at least 80 types of autoimmune diseases. Nearly any body part can be involved. Common symptoms include low grade fever and feeling tired.
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For Research Use Only. Not For Clinical Use.