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Creative Biolabs has successfully developed SARS, MERS and other anti-viral antibodies in the past to assist scientific research. Facing the difficult COVID-19, Creative Biolabs actively invests in antibody development to assist researchers to better understand the characteristics of 2019-nCoV and drug development. Currently, Creative Biolabs is proud to offer an extensive line of research antibodies to support the study of SARS-CoV-2/COVID-19, several of which were validated using virus-infected cell lysates.

Explore the collections of SARS-CoV-2/COVID-19 antibodies >

Human Cytokine Antibody Array (Glass Slide, 54 Targets) (Y0050) (AbAr-0050-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The Human Cytokine Antibody Array detects 54 Human Cytokines. It is suitable for all liquid sample types.
Specifications
Target Array
Cytokine Array
Target
Activin A, Cardiotrophin-1 (CT-1), ErbB3, IL-1 R2, MMP-3, IL-9, L-Selectin (CD62L), MPIF-1 (CCL23), PDGF R beta, Siglec-5 (CD170), TIMP-4, ALCAM (CD166), CD14, E-Selectin, IL-10 R beta, IL-2 R beta (CD122), IP-10 (CXCL10), M-CSF R, NGFR (TNFRSF16), PECAM-1 (CD31), TGF alpha, VE-Cadherin (CDH5), CD80 (B7-1), CXCL16, Fas Ligand (TNFSF6), IL-13 R alpha 2, IL-2 R gamma (Common gamma Chain), LAP/TGF beta 1, MMP-1, PDGF-AA, Prolactin, TGF beta 2, VEGFR2, BMP-5, DR6 (TNFRSF21), ICAM-2 (CD102), IL-18 BP alpha, IL-21 R, Leptin R, MMP-13, PDGF-AB, SCF R (CD117/c-kit), Tie-1, VEGFR3, BMP-7, Endoglin (CD105), IGF-2, IL-18 R beta (AcPL), IL-5 R alpha, LIF, MMP-9, PDGF R alpha, SDF-1 beta (CXCL12 beta), Tie-2
Species Reactivity
Human
Sample Type
Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates
Applications
Application
Multiplex Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Glass Slide
Detection Assay
Sandwich-based
Protocol Outline
Dry the glass slide
Block array surface
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis
Components
Antibody Array Slide
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.
Background
Introduction
Cytokines are a broad and loose category of small proteins (5–20 kDa) that are important in cell signaling. Cytokines are peptides, and cannot cross the lipid bilayer of cells to enter the cytoplasm. Cytokines have been shown to be involved in autocrine signaling, paracrine signaling and endocrine signaling as immunomodulating agents. Their definite distinction from hormones is still part of ongoing research. Cytokines include chemokines, interferons, interleukins, lymphokines, and tumour necrosis factors, but generally not hormones or growth factors (despite some overlap in the terminology). Cytokines are produced by a broad range of cells, including immune cells like macrophages, B lymphocytes, T lymphocytes and mast cells, as well as endothelial cells, fibroblasts, and various stromal cells; a given cytokine may be produced by more than one type of cell.
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