Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Human EGFR Phosphorylation Antibody Array (Membrane, 17 Targets) (Y0044) (AbAr-0044-YC)


See all EGFR Phosphorylation antibodies
Online Inquiry

Specifications

Target Array
EGFR Phosphorylation Array
Target
EGFR (pTyr845), EGFR (pTyr1148), ErbB2 (pTyr1112), ErbB3 (pTyr1289), EGFR (pTyr992), EGFR (pTyr1173), ErbB2 (pTyr1221/1222), ErbB4 (pTyr1284), EGFR (pTyr1045), EGFR (pSer1046/1047), ErbB2 (pTyr1248), EGFR (pTyr1068), EGFR (pSer1070), ErbB2 (pThr686), EGFR (pTyr1086), ErbB2 (pTyr877), ErbB2 (pSer1113)
Species Reactivity
Human
Sample Type
Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates

Applications

Application
Multiplex Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Membrane
Detection Assay
Sandwich-based
Protocol Outline
Apply a blocking agent to the membranes.
Proceed with sample incubation.
Incubate using the cocktail of biotinylated detection antibodies.
Incubate with streptavidin conjugated to horseradish peroxidase (HRP).
Conduct incubation with the detection buffers.
Utilize a chemiluminescent imaging system for image capture.
Execute densitometric evaluation and perform data analysis.
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction
The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family (EGF family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). In many cancer types, mutations affecting EGFR expression or activity could result in cancer. Deficient signaling of the EGFR and other receptor tyrosine kinases in humans is associated with diseases such as Alzheimer's, while over-expression is associated with the development of a wide variety of tumors. Interruption of EGFR signalling, either by blocking EGFR binding sites on the extracellular domain of the receptor or by inhibiting intracellular tyrosine kinase activity, can prevent the growth of EGFR-expressing tumours and improve the patient's condition.
More Infomation
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Human EGFR Phosphorylation Antibody Array (Membrane, 17 Targets) (Y0044)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry