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Human EGFR Phosphorylation Antibody Array (Membrane, 17 Targets) (Y0044) (AbAr-0044-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The Human EGFR Phosphorylation Antibody Array detects 17 site specific and pan Human EGFRs. It is suitable for all liquid sample types but intended for use with cell and tissue lysates.
See all EGFR Phosphorylation antibodies

Specifications

Target Array
EGFR Phosphorylation Array
Target
EGFR (P-Tyr845), EGFR (P-Tyr1148), ErbB2 (P-Tyr1112), ErbB3 (P-Tyr1289), EGFR (P-Tyr992), EGFR (P-Tyr1173), ErbB2 (P-Tyr 1221/1222), ErbB4 (P-Tyr1284), EGFR (P-Tyr1045), EGFR (P-Ser1046/1047), ErbB2 (P-Tyr1248), EGFR (P-Tyr1068), EGFR (P-Ser1070), ErbB2 (P-Thr686), EGFR (P-Tyr1086), ErbB2 (P-Tyr877), ErbB2 (P-Ser1113)
Species Reactivity
Human
Sample Type
Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates

Applications

Application
Multiplex Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Membrane
Detection Assay
Sandwich-based
Protocol Outline
Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction
The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family (EGF family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). In many cancer types, mutations affecting EGFR expression or activity could result in cancer. Deficient signaling of the EGFR and other receptor tyrosine kinases in humans is associated with diseases such as Alzheimer's, while over-expression is associated with the development of a wide variety of tumors. Interruption of EGFR signalling, either by blocking EGFR binding sites on the extracellular domain of the receptor or by inhibiting intracellular tyrosine kinase activity, can prevent the growth of EGFR-expressing tumours and improve the patient's condition.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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