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PPP1CA (1H1) Mouse mAb (CBMAB-Z0523-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
1H1-F7-D8
Antibody Isotype
IgG1
Application
WB, ICC

Basic Information

Immunogen
Purified recombinant human PPP1A protein fragments expressed in E.coli.
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
protein phosphatase 1 catalytic subunit alpha
Entrez Gene ID
5499
UniProt ID
P62136
Alternative Names
PP-1A; PPP1A; PP1alpha
Function
Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Regulates NEK2 function in terms of kinase activity and centrosome number and splitting, both in the presence and absence of radiation-induced DNA damage. Regulator of neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208).
Dephosphorylates CENPA (PubMed:25556658).
Dephosphorylates the 'Ser-139' residue of ATG16L1 causing dissociation of ATG12-ATG5-ATG16L1 complex, thereby inhibiting autophagy (PubMed:26083323).
(Microbial infection) Necessary for alphaviruses replication.
Biological Process
Beta-catenin destruction complex disassemblyISS:ParkinsonsUK-UCL
Branching morphogenesis of an epithelial tubeIEA:Ensembl
Cell cycleIEA:UniProtKB-KW
Cell divisionIEA:UniProtKB-KW
Circadian regulation of gene expressionISS:UniProtKB
DephosphorylationManual Assertion Based On ExperimentIDA:CACAO
Entrainment of circadian clock by photoperiodISS:UniProtKB
Glycogen metabolic processIEA:UniProtKB-KW
Lung developmentIEA:Ensembl
Negative regulation of protein bindingISS:ParkinsonsUK-UCL
Peptidyl-serine dephosphorylationManual Assertion Based On ExperimentTAS:ARUK-UCL
Peptidyl-threonine dephosphorylationManual Assertion Based On ExperimentTAS:ARUK-UCL
Positive regulation of extrinsic apoptotic signaling pathway in absence of ligandIEA:Ensembl
Protein dephosphorylationManual Assertion Based On ExperimentIMP:CACAO
Regulation of canonical Wnt signaling pathway1 PublicationIC:ParkinsonsUK-UCL
Regulation of circadian rhythmManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of glycogen biosynthetic processIEA:Ensembl
Regulation of glycogen catabolic processIEA:Ensembl
Regulation of translational initiation by eIF2 alpha dephosphorylationISS:ParkinsonsUK-UCL
Response to lead ionISS:ARUK-UCL
Cellular Location
Cytoplasm
Nucleus
Nucleus, nucleoplasm
Nucleus, nucleolus
Primarily nuclear and largely excluded from the nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. NOM1 plays a role in targeting this protein to the nucleolus. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles. Shuttles toward the cytosol during infection with VEEV (PubMed:29769351).
PTM
Phosphorylated. Dephosphorylated at Thr-320 in the presence of ionizing radiation.
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For research use only. Not intended for any clinical use.

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