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Rabbit Anti-ABCG1 Recombinant Antibody (V2-12490) (CBMAB-0980-CN)

This product is a rabbit antibody that recognizes ABCG1 of human. The antibody EP1366Y can be used for immunoassay techniques such as: IF, WB.
See all ABCG1 antibodies
Published Data
Rabbit Anti-ABCG1 Recombinant Antibody (EP1366Y) (CBMAB-0980-CN) in IF
Immunofluorescence staining of ABCG1. ...View More
Palmer, M. A., Smart, E., & Haslam, I. S. (2021). Localisation and regulation of cholesterol transporters in the human hair follicle: Mapping changes across the hair cycle. Histochemistry and Cell Biology, 155(5), 529-545.
Rabbit Anti-ABCG1 Recombinant Antibody (EP1366Y) (CBMAB-0980-CN) in WB
Western blot analysis of ABCG1 in C6 cells with 0, 5μM 27-OHC for 24 h. ...View More
Wang, Y., Zhang, X., Wang, T., Liu, W., Wang, L., Hao, L., ... & Xiao, R. (2020). 27-Hydroxycholesterol promotes the transfer of astrocyte-derived cholesterol to neurons in co-cultured SH-SY5Y cells and C6 cells. Frontiers in Cell and Developmental Biology, 8, 580599.

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat, Cattle
Clone
V2-12490
Antibody Isotype
IgG
Application
IF, WB

Basic Information

Immunogen
A synthetic peptide corresponding to residues on human ABCG1
Specificity
Human, Mouse, Rat, Cattle
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100
IF(ICC)1:1,000-1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 40% Glycerol, 0.05% BSA
Preservative
0.01% sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ATP Binding Cassette Subfamily G Member 1
Introduction
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. It is involved in macrophage cholesterol and phospholipids transport, and may regulate cellular lipid homeostasis in other cell types. Six alternative splice variants have been identified. The protein is an active component of the macrophage lipid export complex. It could also be involved in intracellular lipid transport processes. Its role in cellular lipid homeostasis may not be limited to macrophages.
Entrez Gene ID
Human9619
Mouse11307
Rat85264
Cattle510745
UniProt ID
HumanP45844
MouseQ64343
RatQ9EPG9
CattleA0A3Q1M833
Alternative Names
ABC8; WHITE1
Function
Catalyzes the efflux of phospholipids such as sphingomyelin, cholesterol and its oxygenated derivatives like 7beta-hydroxycholesterol and this transport is coupled to hydrlysis of ATP. The lipid efflux is ALB-dependent. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. Prevents cell death by transporting cytotoxic 7beta-hydroxycholesterol.
Biological Process
Amyloid precursor protein catabolic process
Cholesterol efflux
Cholesterol homeostasis
Cholesterol metabolic process
Glycoprotein transport
High-density lipoprotein particle remodeling
Intracellular cholesterol transport
Intracellular receptor signaling pathway
Lipid transport
Low-density lipoprotein particle remodeling
Negative regulation of cholesterol storage
Negative regulation of macrophage derived foam cell differentiation
Phospholipid efflux
Phospholipid homeostasis
Positive regulation of amyloid-beta formation
Positive regulation of cholesterol biosynthetic process
Positive regulation of protein secretion
Regulation of cholesterol esterification
Response to lipid
Response to organic substance
Reverse cholesterol transport
Cellular Location
Golgi apparatus membrane; Cell membrane; Endoplasmic reticulum membrane. Predominantly localized in the intracellular compartments mainly associated with the endoplasmic reticulum (ER) and Golgi membranes.
Topology
Cytoplasmic: 1-426 aa
Helical: 427-445 aa
Extracellular: 446-456 aa
Helical: 457-477 aa
Cytoplasmic: 478-506 aa
Helical: 507-525 aa
Extracellular: 526-533 aa
Helical: 534-555 aa
Cytoplasmic: 556-567 aa
Helical: 568-586 aa
Extracellular: 587-649 aa
Helical: 650-669 aa
Cytoplasmic: 670-678 aa
PTM
Palmitoylation at Cys-315 seems important for trafficking from the endoplasmic reticulum.

Song, X., Yan, G., Wang, H., & Lou, D. (2021). Septin 4 activates PPARγ/LXRα signaling by upregulating ABCA1 and ABCG1 expression to inhibit the formation of THP‑1 macrophage‑derived foam cells. Experimental and therapeutic medicine, 22(1), 1-7.

Meng, F., Xiao, Y., Xie, L., Liu, Q., & Qian, K. (2021). Diagnostic and prognostic value of ABC transporter family member ABCG1 gene in clear cell renal cell carcinoma. Channels, 15(1), 375-385.

Yao, X., & Levine, S. J. (2021). Scavenger Hunt: SR-B1, Adrenal Insufficiency, IL-17A, and Neutrophilic Airway Inflammation in Asthma.

Dong, M., Zhang, Y., Xu, C., Wang, C., Liu, M., Zhang, Z., ... & Zhou, J. (2020). Interferon‐γ decreases ATP‐binding cassette subfamily G member 1‐mediated cholesterol efflux through small ubiquitin‐like modifier/ubiquitin‐dependent liver X receptor‐α degradation in macrophages. Biotechnology and Applied Biochemistry.

Barilla, S., Liang, N., Mileti, E., Ballaire, R., Lhomme, M., Ponnaiah, M., ... & Treuter, E. (2020). Loss of G protein pathway suppressor 2 in human adipocytes triggers lipid remodeling by upregulating ATP binding cassette subfamily G member 1. Molecular metabolism, 42, 101066.

Liu, L., Zhao, L., Chen, P., Cai, H., Hou, Z., Jin, X., ... & Qin, Y. (2020). ATP binding cassette transporters ABCG1 and ABCG16 affect reproductive development via auxin signalling in Arabidopsis. The Plant Journal, 102(6), 1172-1186.

Li, H. N., Zhao, X., Zha, Y. J., Du, F., Liu, J., & Sun, L. (2019). miR‑146a‑5p suppresses ATP‑binding cassette subfamily G member 1 dysregulation in patients with refractory Mycoplasma pneumoniae via interleukin 1 receptor‑associated kinase 1 downregulation. International journal of molecular medicine, 44(6), 2003-2014.

Hou, L., Tang, S., Wu, B. J., Ong, K. L., Westerterp, M., Barter, P. J., ... & Rye, K. A. (2019). Apolipoprotein A‐l improves pancreatic β‐cell function independent of the ATP‐binding cassette transporters ABCA1 and ABCG1. The FASEB Journal, 33(7), 8479-8489.

Pandzic, E., Gelissen, I. C., Whan, R., Barter, P. J., Sviridov, D., Gaus, K., ... & Cochran, B. J. (2017). The ATP binding cassette transporter, ABCG1, localizes to cortical actin filaments. Scientific reports, 7(1), 1-10.

Wang, L., Fumoto, T., Masumoto, S., Shoji, T., Miura, T., Naraoka, M., ... & Ohkuma, H. (2017). Regression of atherosclerosis with apple procyanidins by activating the ATP-binding cassette subfamily A member 1 in a rabbit model. Atherosclerosis, 258, 56-64.

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For research use only. Not intended for any clinical use.

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