Mouse Anti-ASPH Recombinant Antibody (A10) (CBMAB-1152-CN)

Name: Mouse Anti-ASPH Recombinant Antibody (A10)
SKU: CBMAB-1152-CN
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ASPH Recombinant Antibody (A10) CBMAB-1152-CN in WB

Specific ASPH siRNAs visibly downregulate ASPH expression after 18 h. ...View More

Benelli, R., Costa, D., Mastracci, L., Grillo, F., Olsen, M. J., Barboro, P., ... & Ferrari, N. (2020). Aspartate-β-hydroxylase: a promising target to limit the local invasiveness of colorectal cancer. Cancers, 12(4), 971.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

A10

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 382-681 mapping within an internal region of ASPH of human origin.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

aspartate beta-hydroxylase

Introduction

This gene is thought to play an important role in calcium homeostasis. The gene is expressed from two promoters and undergoes extensive alternative splicing. The encoded set of proteins share varying amounts of overlap near their N-termini but have substantial variations in their C-terminal domains resulting in distinct functional properties. ASPH is thought to play an important role in calcium homeostasis. Alternative splicing of this gene results in five transcript variants which vary in protein translation, the coding of catalytic domains, and tissue expression.

Entrez Gene ID

444

UniProt ID

Q12797

Alternative Names

AAH; BAH; HAAH; JCTN; FDLAB; junctin; CASQ2BP1

Function

Isoform 1: specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins.
Isoform 8: membrane-bound Ca2+-sensing protein, which is a structural component of the ER-plasma membrane junctions. Isoform 8 regulates the activity of Ca(+2) released-activated Ca(+2) (CRAC) channels in T-cells.

Biological Process

Activation of cysteine-type endopeptidase activity Source: BHF-UCL
Activation of store-operated calcium channel activity Source: UniProtKB
Calcium ion homeostasis Source: UniProtKB
Calcium ion transmembrane transport Source: UniProtKB
Cellular response to calcium ion Source: UniProtKB
Detection of calcium ion Source: BHF-UCL
Ion transmembrane transport Source: Reactome
Muscle contraction Source: ProtInc
Peptidyl-aspartic acid hydroxylation Source: GO_Central
Positive regulation of calcium ion transport into cytosol Source: UniProtKB
Positive regulation of intracellular protein transport Source: UniProtKB
Positive regulation of proteolysis Source: BHF-UCL
Positive regulation of ryanodine-sensitive calcium-release channel activity Source: BHF-UCL
Positive regulation of transcription, DNA-templated Source: UniProtKB
Regulation of cardiac conduction Source: Reactome
Regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion Source: BHF-UCL
Regulation of cell communication by electrical coupling Source: BHF-UCL
Regulation of inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity Source: UniProtKB
Regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum Source: BHF-UCL
Regulation of ryanodine-sensitive calcium-release channel activity Source: BHF-UCL
Response to ATP Source: UniProtKB

Cellular Location

Isoform 1: Endoplasmic reticulum membrane
Isoform 4: Sarcoplasmic reticulum membrane
Isoform 8: Endoplasmic reticulum membrane

Involvement in disease

Facial dysmorphism, lens dislocation, anterior segment abnormalities, and spontaneous filtering blebs (FDLAB): A syndrome characterized by dislocated crystalline lenses and anterior segment abnormalities in association with a distinctive facies involving flat cheeks and a beaked nose. Some affected individuals develop highly unusual non-traumatic conjunctival cysts (filtering blebs).

Topology

Cytoplasmic: 1-53 aa
Helical: 54-74 aa
Lumenal: 75-758 aa

Nagaoka, K., Ogawa, K., Ji, C., Cao, K. Y., Bai, X., Mulla, J., ... & Huang, C. K. (2021). Targeting aspartate beta-hydroxylase with the small molecule inhibitor MO-I-1182 suppresses cholangiocarcinoma metastasis. Digestive Diseases and Sciences, 66, 1080-1089.

Senthil, S., Sharma, S., Vishwakarma, S., & Kaur, I. (2021). A novel mutation in the aspartate beta-hydroxylase (ASPH) gene is associated with a rare form of Traboulsi syndrome. Ophthalmic Genetics, 42(1), 28-34.

Kanwal, M., Smahel, M., Olsen, M., Smahelova, J., & Tachezy, R. (2020). Aspartate β-hydroxylase as a target for cancer therapy. Journal of Experimental & Clinical Cancer Research, 39(1), 1-12.

Zheng, W., Wang, X., Hu, J., Bai, B., & Zhu, H. (2020). Diverse molecular functions of aspartate β‑hydroxylase in cancer. Oncology Reports.

Benelli, R., Costa, D., Mastracci, L., Grillo, F., Olsen, M. J., Barboro, P., ... & Ferrari, N. (2020). Aspartate-β-hydroxylase: A promising target to limit the local invasiveness of colorectal cancer. Cancers, 12(4), 971.

Barboro, P., Benelli, R., Tosetti, F., Costa, D., Capaia, M., Astigiano, S., ... & Ferrari, N. (2020). Aspartate β-hydroxylase targeting in castration-resistant prostate cancer modulates the NOTCH/HIF1α/GSK3β crosstalk. Carcinogenesis, 41(9), 1246-1252.

Nagaoka, K., Bai, X., Ogawa, K., Dong, X., Zhang, S., Zhou, Y., ... & Wands, J. R. (2019). Anti-tumor activity of antibody drug conjugate targeting aspartate-β-hydroxylase in pancreatic ductal adenocarcinoma. Cancer letters, 449, 87-98.

Holtzman, N. G., Lebowitz, M. S., Koka, R., Baer, M. R., Malhotra, K., Fuller, S. A., ... & Emadi, A. (2018). Aspartate Beta-Hydroxylase (ASPH) As a Novel Therapeutic Target in Acute Myeloid Leukemia. Blood, 132, 5273.

Huang, C. K., Iwagami, Y., Zou, J., Casulli, S., Lu, S., Nagaoka, K., ... & Wands, J. R. (2018). Aspartate beta-hydroxylase promotes cholangiocarcinoma progression by modulating RB1 phosphorylation. Cancer letters, 429, 1-10.

Zou, Q., Hou, Y., Wang, H., Wang, K., Xing, X., Xia, Y., ... & Shen, F. (2018). Hydroxylase activity of ASPH promotes hepatocellular carcinoma metastasis through epithelial-to-mesenchymal transition pathway. EBioMedicine, 31, 287-298.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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