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Mouse Anti-BPTF Recombinant Antibody (CBT232) (V2LY-0625-LY3683)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBT232
Antibody Isotype
IgG
Application
WB, ELISA

Basic Information

Immunogen
Purified recombinant fragment of human BPTF expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
ELISA1:10,000
WB1:500-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Buffer
BSA, Glycerol
Preservative
Sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Bromodomain PHD Finger Transcription Factor
Entrez Gene ID
2186
UniProt ID
Q12830
Function
Histone-binding component of NURF (nucleosome-remodeling factor), a complex which catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin. Specifically recognizes H3 tails trimethylated on 'Lys-4' (H3K4me3), which mark transcription start sites of virtually all active genes. May also regulate transcription through direct binding to DNA or transcription factors.
Biological Process
Anterior/posterior pattern specification Source: Ensembl
Brain development Source: HGNC-UCL
Cellular response to nerve growth factor stimulus Source: Ensembl
Chromatin remodeling Source: HGNC-UCL
Embryonic placenta development Source: Ensembl
Endoderm development Source: Ensembl
Negative regulation of transcription by RNA polymerase II Source: MGI
Nucleosome mobilization Source: GO_Central
Positive regulation of transcription by RNA polymerase II Source: HGNC-UCL
Regulation of transcription by RNA polymerase II Source: HGNC-UCL
Response to wounding Source: Ensembl
Cellular Location
Nucleus; Cytoplasm. In brains of Alzheimer disease patients, present in a subset of amyloid-containing plaques.
Involvement in disease
Neurodevelopmental disorder with dysmorphic facies and distal limb anomalies (NEDDFL): An autosomal dominant neurodevelopmental disorder characterized by variable degrees of developmental delay, intellectual disability, speech delay, postnatal microcephaly, dysmorphic features, and mild abnormalities of the hands and feet.
PTM
Phosphorylation enhances DNA-binding.
Highly susceptible to proteolysis.
More Infomation

Zahid, H., Olson, N. M., & Pomerantz, W. C. (2021). Opportunity knocks for uncovering the new function of an understudied nucleosome remodeling complex member, the bromodomain PHD finger transcription factor, BPTF. Current opinion in chemical biology, 63, 57-67.

Miao, J., Zhang, M., Huang, X., Xu, L., Tang, R., Wang, H., & Han, S. (2021). Upregulation of bromodomain PHD finger transcription factor in ovarian cancer and its critical role for cancer cell proliferation and survival. Biochemistry and Cell Biology, 99(999), 1-9.

Mélin, L., Calosing, C., Kharenko, O. A., Hansen, H. C., & Gagnon, A. (2021). Synthesis of NVS-BPTF-1 and evaluation of its biological activity. Bioorganic & Medicinal Chemistry Letters, 128208.

Xiong, L., Mao, X., Guo, Y., Zhou, Y., Chen, M., Chen, P., ... & Li, L. (2021). Discovery of selective BPTF bromodomain inhibitors by screening and structure-based optimization. Biochemical and Biophysical Research Communications, 545, 125-131.

Lu, T., Lu, H., Duan, Z., Wang, J., Han, J., Xiao, S., ... & Luo, C. (2021). Discovery of High-Affinity Inhibitors of the BPTF Bromodomain. Journal of Medicinal Chemistry, 64(16), 12075-12088.

Zahid, H., Buchholz, C. R., Singh, M., Ciccone, M. F., Chan, A., Nithianantham, S., ... & Pomerantz, W. C. (2021). New Design Rules for Developing Potent Cell-Active Inhibitors of the Nucleosome Remodeling Factor (NURF) via BPTF Bromodomain Inhibition. Journal of Medicinal Chemistry.

Pan, Y., Yuan, F., Li, Y., Wang, G., Lin, Z., & Chen, L. (2019). Bromodomain PHD‑finger transcription factor promotes glioma progression and indicates poor prognosis. Oncology reports, 41(1), 246-256.

Zhang, D., Han, J., Lu, W., Lian, F., Wang, J., Lu, T., ... & Luo, C. (2019). Discovery of alkoxy benzamide derivatives as novel BPTF bromodomain inhibitors via structure-based virtual screening. Bioorganic chemistry, 86, 494-500.

Kirberger, S. E., Ycas, P. D., Johnson, J. A., Chen, C., Ciccone, M. F., Woo, R. W., ... & Pomerantz, W. C. (2019). Selectivity, ligand deconstruction, and cellular activity analysis of a BPTF bromodomain inhibitor. Organic & biomolecular chemistry, 17(7), 2020-2027.

Perell, G. T., Mishra, N. K., Sudhamalla, B., Ycas, P. D., Islam, K., & Pomerantz, W. C. (2017). Specific acetylation patterns of H2A. Z form transient interactions with the BPTF bromodomain. Biochemistry, 56(35), 4607-4615.

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For research use only. Not intended for any clinical use.

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