Mouse Anti-BPTF Recombinant Antibody (CBYY-1993) (CBMAB-2342-YY)

Name: Mouse Anti-BPTF Recombinant Antibody (CBYY-1993)
SKU: CBMAB-2342-YY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-1993

Application

Immunogen

Purified recombinant fragment of human BPTF expressed in E. coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:500-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Preservative

0.03% sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Bromodomain PHD Finger Transcription Factor

Introduction

This gene was identified by the reactivity of its encoded protein to a monoclonal antibody prepared against brain homogenates from patients with Alzheimer's disease. Analysis of the original protein (fetal Alz-50 reactive clone 1, or FAC1), identified as an 810 aa protein containing a DNA-binding domain and a zinc finger motif, suggested it might play a role in the regulation of transcription. High levels of FAC1 were detected in fetal brain and in patients with neurodegenerative diseases. The protein encoded by this gene is actually much larger than originally thought, and it also contains a C-terminal bromodomain characteristic of proteins that regulate transcription during proliferation. The encoded protein is highly similar to the largest subunit of the Drosophila NURF (nucleosome remodeling factor) complex. In Drosophila, the NURF complex, which catalyzes nucleosome sliding on DNA and interacts with sequence-specific transcription factors, is necessary for the chromatin remodeling required for transcription. Two alternative transcripts encoding different isoforMouse have been described completely.

Entrez Gene ID

2186

UniProt ID

Q12830

Alternative Names

Bromodomain PHD Finger Transcription Factor; Fetal Alzheimer Antigen; Bromodomain And PHD Finger-Containing Transcription Factor; Fetal Alz-50 Clone 1 Protein; FALZ; FAC1; Bromodomain And PHD Domain Transcription Factor; Nucleosome Remodeling Factor, Large Subunit;

Function

Histone-binding component of NURF (nucleosome-remodeling factor), a complex which catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin. Specifically recognizes H3 tails trimethylated on 'Lys-4' (H3K4me3), which mark transcription start sites of virtually all active genes. May also regulate transcription through direct binding to DNA or transcription factors.

Biological Process

Anterior/posterior pattern specification Source: Ensembl
Brain development Source: HGNC-UCL
Cellular response to nerve growth factor stimulus Source: Ensembl
Chromatin remodeling Source: HGNC-UCL
Embryonic placenta development Source: Ensembl
Endoderm development Source: Ensembl
Negative regulation of transcription by RNA polymerase II Source: MGI
Nucleosome mobilization Source: GO_Central
Positive regulation of transcription by RNA polymerase II Source: HGNC-UCL
Regulation of transcription by RNA polymerase II Source: HGNC-UCL
Response to wounding Source: Ensembl

Cellular Location

Nucleus; Cytoplasm. In brains of Alzheimer disease patients, present in a subset of amyloid-containing plaques.

Involvement in disease

Neurodevelopmental disorder with dysmorphic facies and distal limb anomalies (NEDDFL): An autosomal dominant neurodevelopmental disorder characterized by variable degrees of developmental delay, intellectual disability, speech delay, postnatal microcephaly, dysmorphic features, and mild abnormalities of the hands and feet.

PTM

Phosphorylation enhances DNA-binding.
Highly susceptible to proteolysis.

Zahid, H., Olson, N. M., & Pomerantz, W. C. (2021). Opportunity knocks for uncovering the new function of an understudied nucleosome remodeling complex member, the bromodomain PHD finger transcription factor, BPTF. Current opinion in chemical biology, 63, 57-67.

Miao, J., Zhang, M., Huang, X., Xu, L., Tang, R., Wang, H., & Han, S. (2021). Upregulation of bromodomain PHD finger transcription factor in ovarian cancer and its critical role for cancer cell proliferation and survival. Biochemistry and Cell Biology, 99(999), 1-9.

Mélin, L., Calosing, C., Kharenko, O. A., Hansen, H. C., & Gagnon, A. (2021). Synthesis of NVS-BPTF-1 and evaluation of its biological activity. Bioorganic & Medicinal Chemistry Letters, 128208.

Xiong, L., Mao, X., Guo, Y., Zhou, Y., Chen, M., Chen, P., ... & Li, L. (2021). Discovery of selective BPTF bromodomain inhibitors by screening and structure-based optimization. Biochemical and Biophysical Research Communications, 545, 125-131.

Lu, T., Lu, H., Duan, Z., Wang, J., Han, J., Xiao, S., ... & Luo, C. (2021). Discovery of High-Affinity Inhibitors of the BPTF Bromodomain. Journal of Medicinal Chemistry, 64(16), 12075-12088.

Zahid, H., Buchholz, C. R., Singh, M., Ciccone, M. F., Chan, A., Nithianantham, S., ... & Pomerantz, W. C. (2021). New Design Rules for Developing Potent Cell-Active Inhibitors of the Nucleosome Remodeling Factor (NURF) via BPTF Bromodomain Inhibition. Journal of Medicinal Chemistry.

Pan, Y., Yuan, F., Li, Y., Wang, G., Lin, Z., & Chen, L. (2019). Bromodomain PHD‑finger transcription factor promotes glioma progression and indicates poor prognosis. Oncology reports, 41(1), 246-256.

Zhang, D., Han, J., Lu, W., Lian, F., Wang, J., Lu, T., ... & Luo, C. (2019). Discovery of alkoxy benzamide derivatives as novel BPTF bromodomain inhibitors via structure-based virtual screening. Bioorganic chemistry, 86, 494-500.

Kirberger, S. E., Ycas, P. D., Johnson, J. A., Chen, C., Ciccone, M. F., Woo, R. W., ... & Pomerantz, W. C. (2019). Selectivity, ligand deconstruction, and cellular activity analysis of a BPTF bromodomain inhibitor. Organic & biomolecular chemistry, 17(7), 2020-2027.

Perell, G. T., Mishra, N. K., Sudhamalla, B., Ycas, P. D., Islam, K., & Pomerantz, W. C. (2017). Specific acetylation patterns of H2A. Z form transient interactions with the BPTF bromodomain. Biochemistry, 56(35), 4607-4615.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
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For research use only. Not intended for any clinical use.

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