Mouse Anti-CCM2 Recombinant Antibody (2E4) (CBMAB-C4319-LY)

Name: Mouse Anti-CCM2 Recombinant Antibody (2E4)
SKU: CBMAB-C4319-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2E4

Application

IF, IHC, WB

Immunogen

Full length human recombinant protein of human CCM2 produced in HEK293T cell.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:2,000
IHC	1:150
IF(ICC)	1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

CCM2 Scaffolding Protein

Introduction

CCM2 (CCM2 Scaffolding Protein) is a Protein Coding gene. Diseases associated with CCM2 include Cerebral Cavernous Malformations 2 and Cavernous Hemangioma. Among its related pathways are p38 MAPK signaling pathway (Pathway Interaction Database) and Regulation of p38-alpha and p38-beta.

An important paralog of this gene is CCM2L.

Entrez Gene ID

83605

UniProt ID

Q9BSQ5

Alternative Names

CCM2 Scaffolding Protein; Malcavernin; Cerebral Cavernous Malformation 2; Osmosensing Scaffold For MEKK3; C7orf22; Cerebral Cavernous Malformations 2 Protein; Chromosome 7 Open Reading Frame 22; PP10187; OSM;

Function

Component of the CCM signaling pathway which is a crucial regulator of heart and vessel formation and integrity. May act through the stabilization of endothelial cell junctions (By similarity).
May function as a scaffold protein for MAP2K3-MAP3K3 signaling. Seems to play a major role in the modulation of MAP3K3-dependent p38 activation induced by hyperosmotic shock (By similarity).

Biological Process

Blood vessel endothelial cell differentiation Source: Ensembl
Cell-cell junction organization Source: Ensembl
Endothelial cell development Source: Ensembl
Endothelial tube morphogenesis Source: MGI
Inner ear development Source: Ensembl
Integrin-mediated signaling pathway Source: UniProtKB
In utero embryonic development Source: Ensembl
Multicellular organism growth Source: Ensembl
Pericardium development Source: Ensembl
Stress-activated MAPK cascade Source: UniProtKB
Vasculogenesis Source: UniProtKB
Venous blood vessel morphogenesis Source: Ensembl

Cellular Location

Cytoplasm

Involvement in disease

Cerebral cavernous malformations 2 (CCM2): A congenital vascular anomaly of the central nervous system that can result in hemorrhagic stroke, seizures, recurrent headaches, and focal neurologic deficits. The lesions are characterized by grossly enlarged blood vessels consisting of a single layer of endothelium and without any intervening neural tissue, ranging in diameter from a few millimeters to several centimeters.

Ishii, K., Tozaka, N., Tsutsumi, S., Muroi, A., & Tamaoka, A. (2020). Familial cerebral cavernous malformation presenting with epilepsy caused by mutation in the CCM2 gene: A case report. Medicine, 99(29).

Jiang, X., Padarti, A., Qu, Y., Sheng, S., Abou-Fadel, J., Badr, A., & Zhang, J. (2019). Alternatively spliced isoforms reveal a novel type of PTB domain in CCM2 protein. Scientific reports, 9(1), 1-19.

Citron, Y. R. (2018). The Small Conserved CM2 Domain in Centrosomin is a Multi-functional Binding Domain that Drives Protein Organization in the Centrosome (Doctoral dissertation, UCSF).

Lisowska, J., Rödel, C. J., Manet, S., Miroshnikova, Y. A., Boyault, C., Planus, E., ... & Faurobert, E. (2018). The CCM1–CCM2 complex controls complementary functions of ROCK1 and ROCK2 that are required for endothelial integrity. Journal of cell science, 131(15), jcs216093.

Miura, K., Nojiri, T., Akitake, Y., Ando, K., Fukuhara, S., Zenitani, M., ... & Kangawa, K. (2017). CCM2 and PAK4 act downstream of atrial natriuretic peptide signaling to promote cell spreading. Biochemical Journal, 474(11), 1897-1918.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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