Mouse Anti-CES1 Recombinant Antibody (CBFYC-1732) (CBMAB-C1794-FY)

Name: Mouse Anti-CES1 Recombinant Antibody (CBFYC-1732)
SKU: CBMAB-C1794-FY
Rating: 5 (1 reviews)

See all CES1 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYC-1732

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 261-355 mapping within an internal region of CES1 of human.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 19-563

More Infomation

Target

Full Name

Carboxylesterase 1

Introduction

This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of this gene cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for this gene.

Entrez Gene ID

1066

UniProt ID

P23141

Alternative Names

Carboxylesterase 1; Carboxylesterase 1 (Monocyte/Macrophage Serine Esterase 1); Human Monocyte/Macrophage Serine Esterase 1; Methylumbelliferyl-Acetate Deacetylase 1; Monocyte/Macrophage Serine Esterase; Brain Carboxylesterase HBr1; Triacylglycerol Hydrolase; Cocaine Carboxylesterase; Retinyl Ester Hydrolase; Serine Esterase 1; EC 3.1.1.1; Egasyn; HCE-1; ACAT; CE-1; CES2

Function

Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs (PubMed:7980644, PubMed:9169443, PubMed:9490062, PubMed:18762277).
Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester (PubMed:7980644, PubMed:9169443, PubMed:9490062, PubMed:18762277).
Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine (PubMed:7980644).
Catalyzes the transesterification of cocaine to form cocaethylene (PubMed:7980644).
Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate (PubMed:7980644).
Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes of 2-arachidonoylglycerol and prostaglandins (PubMed:21049984).
Hydrolyzes cellular cholesteryl esters to free cholesterols and promotes reverse cholesterol transport (RCT) by facilitating both the initial and final steps in the process (PubMed:18762277, PubMed:16024911, PubMed:11015575, PubMed:16971496).
First of all, allows free cholesterol efflux from macrophages to extracellular cholesterol acceptors and secondly, releases free cholesterol from lipoprotein-delivered cholesteryl esters in the liver for bile acid synthesis or direct secretion into the bile (PubMed:18762277, PubMed:18599737, PubMed:16971496).

Biological Process

Angiotensin maturation Source: Reactome
Cellular response to cholesterol Source: ARUK-UCL
Cellular response to low-density lipoprotein particle stimulus Source: ARUK-UCL
Cholesterol biosynthetic process Source: BHF-UCL
Cholesterol ester hydrolysis involved in cholesterol transport Source: BHF-UCL
Cholesterol homeostasis Source: ARUK-UCL
Cholesterol metabolic process Source: UniProtKB
Epithelial cell differentiation Source: UniProtKB
Lipid catabolic process Source: GO_Central
Medium-chain fatty acid metabolic process Source: BHF-UCL
Negative regulation of cholesterol storage Source: UniProtKB
Positive regulation of cholesterol efflux Source: UniProtKB
Positive regulation of cholesterol metabolic process Source: UniProtKB
Regulation of bile acid biosynthetic process Source: UniProtKB
Regulation of bile acid secretion Source: UniProtKB
Response to toxic substance Source: ProtInc
Reverse cholesterol transport Source: UniProtKB
Xenobiotic metabolic process Source: Reactome

Cellular Location

Cytoplasm; Endoplasmic reticulum lumen; Lipid droplet. Moves from cytoplasm to lipid droplets upon lipid loading. Associates with lipid droplets independently of triglycerides (TG) content of the droplets and hydrolyzes cholesteryl esters more efficiently from mixed droplets.

PTM

Contains sialic acid.
Cleavage of the signal sequence can occur at 2 positions, either between Trp-17 and Gly-18 or between Gly-18 and His-19.

Na, K., Kim, M., Kim, C. Y., Lim, J. S., Cho, J. Y., Shin, H., ... & Paik, Y. K. (2020). Potential Regulatory Role of Human-Carboxylesterase-1 Glycosylation in Liver Cancer Cell Growth. Journal of Proteome Research, 19(12), 4867-4883.

Her, L., & Zhu, H. J. (2020). Carboxylesterase 1 and precision pharmacotherapy: pharmacogenetics and nongenetic regulators. Drug Metabolism and Disposition, 48(3), 230-244.

Xu, Y., Zhu, Y., Bawa, F. C., Hu, S., Pan, X., Yin, L., & Zhang, Y. (2020). Hepatocyte‐Specific Expression of Human Carboxylesterase 1 Attenuates Diet‐Induced Steatohepatitis and Hyperlipidemia in Mice. Hepatology communications, 4(4), 527-539.

Wang, X., Shi, J., & Zhu, H. J. (2019). Functional study of carboxylesterase 1 protein isoforms. Proteomics, 19(4), 1800288.

Ding, L., Tian, Z., Hou, J., Dou, T., Jin, Q., Wang, D., ... & Ge, G. (2019). Sensing carboxylesterase 1 in living systems by a practical and isoform-specific fluorescent probe. Chinese Chemical Letters, 30(3), 558-562.

Tian, Z., Ding, L., Li, K., Song, Y., Dou, T., Hou, J., ... & Cui, J. (2019). Rational design of a long-wavelength fluorescent probe for highly selective sensing of carboxylesterase 1 in living systems. Analytical chemistry, 91(9), 5638-5645.

Qian, Y., Wang, X., & Markowitz, J. S. (2019). In vitro inhibition of carboxylesterase 1 by major cannabinoids and selected metabolites. Drug Metabolism and Disposition, 47(5), 465-472.

Neuvonen, M., Tarkiainen, E. K., Tornio, A., Hirvensalo, P., Tapaninen, T., Paile‐Hyvärinen, M., ... & Niemi, M. (2018). Effects of genetic variants on carboxylesterase 1 gene expression, and clopidogrel pharmacokinetics and antiplatelet effects. Basic & clinical pharmacology & toxicology, 122(3), 341-345.

Xu, J., Xu, Y., Xu, Y., Yin, L., & Zhang, Y. (2017). Global inactivation of carboxylesterase 1 (Ces1/Ces1g) protects against atherosclerosis in Ldlr−/− mice. Scientific reports, 7(1), 1-12.

Wang, X., Rida, N., Shi, J., Wu, A. H., Bleske, B. E., & Zhu, H. J. (2017). A comprehensive functional assessment of carboxylesterase 1 nonsynonymous polymorphisms. Drug Metabolism and Disposition, 45(11), 1149-1155.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-CES1 Recombinant Antibody (CBFYC-1732)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-CES1 Recombinant Antibody (A103) (CAT#: CBMAB-AP11704LY)

Mouse Anti-CES1 Recombinant Antibody (A104) (CAT#: CBMAB-AP11705LY)

Mouse Anti-CES1 Recombinant Antibody (A106) (CAT#: CBMAB-AP11706LY)

Mouse Anti-CES1 Recombinant Antibody (C2550) (CAT#: CBMAB-C2550-LY)

Rabbit Anti-CES1 Recombinant Antibody (A105) (CAT#: CBMAB-AP11707LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry