Mouse Anti-CES1 Recombinant Antibody (A104) (CBMAB-AP11705LY)

Name: Mouse Anti-CES1 Recombinant Antibody (A104)
SKU: CBMAB-AP11705LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

A104

Application

WB, IP

Immunogen

Recombinant human Carboxylesterase1/CES-1 His19-Glu563.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	25 μg/ml
IP	2 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, Trehalose

Preservative

None

Purity

Affinity purity

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Carboxylesterase 1

Introduction

This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of this gene cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

Alternative Names

Carboxylesterase 1; Carboxylesterase 1 (Monocyte/Macrophage Serine Esterase 1); Human Monocyte/Macrophage Serine Esterase 1; Methylumbelliferyl-Acetate Deacetylase 1; Monocyte/Macrophage Serine Esterase; Brain Carboxylesterase HBr1; Triacylglycerol Hydrolase; Cocaine Carboxylesterase; Retinyl Ester Hydrolase; Serine Esterase 1; EC 3.1.1.1; Egasyn; HCE-1; ACAT; CE-1; CES2;

Function

SMC-551S

Biological Process

Angiotensin maturation Source: Reactome
Cellular response to cholesterol Source: ARUK-UCL
Cellular response to low-density lipoprotein particle stimulus Source: ARUK-UCL
Cholesterol biosynthetic process Source: BHF-UCL
Cholesterol ester hydrolysis involved in cholesterol transport Source: BHF-UCL
Cholesterol homeostasis Source: ARUK-UCL
Cholesterol metabolic process Source: UniProtKB
Epithelial cell differentiation Source: UniProtKB
Lipid catabolic process Source: GO_Central
Medium-chain fatty acid metabolic process Source: BHF-UCL
Negative regulation of cholesterol storage Source: UniProtKB
Positive regulation of cholesterol efflux Source: UniProtKB
Positive regulation of cholesterol metabolic process Source: UniProtKB
Regulation of bile acid biosynthetic process Source: UniProtKB
Regulation of bile acid secretion Source: UniProtKB
Response to toxic substance Source: ProtInc
Reverse cholesterol transport Source: UniProtKB
Xenobiotic metabolic process Source: Reactome

Cellular Location

Cytoplasm; Endoplasmic reticulum lumen; Lipid droplet. Moves from cytoplasm to lipid droplets upon lipid loading. Associates with lipid droplets independently of triglycerides (TG) content of the droplets and hydrolyzes cholesteryl esters more efficiently from mixed droplets.

PTM

Contains sialic acid.
Cleavage of the signal sequence can occur at 2 positions, either between Trp-17 and Gly-18 or between Gly-18 and His-19.

Na, K., Kim, M., Kim, C. Y., Lim, J. S., Cho, J. Y., Shin, H., ... & Paik, Y. K. (2020). Potential Regulatory Role of Human-Carboxylesterase-1 Glycosylation in Liver Cancer Cell Growth. Journal of Proteome Research, 19(12), 4867-4883.

Her, L., & Zhu, H. J. (2020). Carboxylesterase 1 and precision pharmacotherapy: pharmacogenetics and nongenetic regulators. Drug Metabolism and Disposition, 48(3), 230-244.

Xu, Y., Zhu, Y., Bawa, F. C., Hu, S., Pan, X., Yin, L., & Zhang, Y. (2020). Hepatocyte‐Specific Expression of Human Carboxylesterase 1 Attenuates Diet‐Induced Steatohepatitis and Hyperlipidemia in Mice. Hepatology communications, 4(4), 527-539.

Wang, X., Shi, J., & Zhu, H. J. (2019). Functional study of carboxylesterase 1 protein isoforms. Proteomics, 19(4), 1800288.

Ding, L., Tian, Z., Hou, J., Dou, T., Jin, Q., Wang, D., ... & Ge, G. (2019). Sensing carboxylesterase 1 in living systems by a practical and isoform-specific fluorescent probe. Chinese Chemical Letters, 30(3), 558-562.

Tian, Z., Ding, L., Li, K., Song, Y., Dou, T., Hou, J., ... & Cui, J. (2019). Rational design of a long-wavelength fluorescent probe for highly selective sensing of carboxylesterase 1 in living systems. Analytical chemistry, 91(9), 5638-5645.

Qian, Y., Wang, X., & Markowitz, J. S. (2019). In vitro inhibition of carboxylesterase 1 by major cannabinoids and selected metabolites. Drug Metabolism and Disposition, 47(5), 465-472.

Neuvonen, M., Tarkiainen, E. K., Tornio, A., Hirvensalo, P., Tapaninen, T., Paile‐Hyvärinen, M., ... & Niemi, M. (2018). Effects of genetic variants on carboxylesterase 1 gene expression, and clopidogrel pharmacokinetics and antiplatelet effects. Basic & clinical pharmacology & toxicology, 122(3), 341-345.

Xu, J., Xu, Y., Xu, Y., Yin, L., & Zhang, Y. (2017). Global inactivation of carboxylesterase 1 (Ces1/Ces1g) protects against atherosclerosis in Ldlr−/− mice. Scientific reports, 7(1), 1-12.

Wang, X., Rida, N., Shi, J., Wu, A. H., Bleske, B. E., & Zhu, H. J. (2017). A comprehensive functional assessment of carboxylesterase 1 nonsynonymous polymorphisms. Drug Metabolism and Disposition, 45(11), 1149-1155.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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