Mouse Anti-CMKLR1 Recombinant Antibody (CBYY-C3187) (CBMAB-C4630-YY)

Name: Mouse Anti-CMKLR1 Recombinant Antibody (CBYY-C3187)
SKU: CBMAB-C4630-YY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CMKLR1 Recombinant Antibody (CBYY-C3187) (CBMAB-C4630-YY) in IHC

SPM receptor localization in normal and injured vascular tissues. Representative images of immunohistochemical staining of normal and injured arteries were demonstrated. Expression of ALX/FPR2 and ERV1/ChemR23 along with CD31, SMA, and CD45 were assessed at different time points; before (a-d), 1 day (e-h) and 7 days (i-l) after angioplasty. Higher magnification images of injured arteries at 7 days with confocal microscopy demonstrated positive cells of ALX/FPR2, ERV1/ChemR23, CD31 and CD45 in the neo-endothelial layer (m-p). Dotted line shows the internal Elastic Lamina which defined the border of the neointimal hyperplasia (NIH). Consecutive sections were used for the immunostaining with each antibody in the tissues. SMA indicated smooth muscle Actin. Scale bar: 100 μm (a-l), 50 μm (m-p). Green = ALX/FPR2 or ERV1/ChemR23 or CD45, Red = CD31 or SMA, Blue = DAPI. ...View More

Kagaya, H., Kim, A. S., Chen, M., Lin, P. Y., Yin, X., Spite, M., & Conte, M. S. (2024). Dynamic changes in proresolving lipid mediators and their receptors following acute vascular injury in male rats. Physiological Reports, 12(15), e16178.

Distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Mouse Anti-CMKLR1 Recombinant Antibody (CBYY-C3187) (CBMAB-C4630-YY) in WB

Expression of chemerin receptors, CMKLR1 and CCRL2, in HUVECs. Chemerin receptors were identified by Western blot analyses using anti-human CMKLR1 and CCRL2 antibodies. ...View More

Nakamura, N., Naruse, K., Kobayashi, Y., Miyabe, M., Saiki, T., Enomoto, A., ... & Matsubara, T. (2018). Chemerin promotes angiogenesis in vivo. Physiological reports, 6(24), e13962.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-C3187

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 273-305 within a C-terminal extracellular domain of ChemR23 of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

0.1% gelatin

Preservative

<0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Chemerin Chemokine-Like Receptor 1

Entrez Gene ID

Human	1240
Mouse	14747
Rat	60669

UniProt ID

Human	Q99788
Mouse	P97468
Rat	O35786

Alternative Names

AHUS5, ARMD9, ASPa, C3b, CPAMD1, HEL-S-62p, C3

Function

Receptor for the chemoattractant adipokine chemerin/RARRES2 and for the omega-3 fatty acid derived molecule resolvin E1. Interaction with RARRES2 induces activation of intracellular signaling molecules, such as SKY, MAPK1/3 (ERK1/2), MAPK14/P38MAPK and PI3K leading to multifunctional effects, like, reduction of immune responses, enhancing of adipogenesis and angionesis. Resolvin E1 down-regulates cytokine production in macrophages by reducing the activation of MAPK1/3 (ERK1/2) and NF-kappa-B. Positively regulates adipogenesis and adipocyte metabolism. Acts as a coreceptor for several SIV strains (SIVMAC316, SIVMAC239, SIVMACL7E-FR and SIVSM62A), as well as a primary HIV-1 strain (92UG024-2).

Biological Process

Chemotaxis Source: UniProtKB
Complement receptor mediated signaling pathway Source: GO_Central
G protein-coupled receptor signaling pathway Source: UniProtKB
Immune response Source: ProtInc
Inflammatory response Source: GO_Central
Negative regulation of interleukin-12 production Source: UniProtKB
Negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
Phospholipase C-activating G protein-coupled receptor signaling pathway Source: GO_Central
Positive regulation of cold-induced thermogenesis Source: YuBioLab
Positive regulation of cytosolic calcium ion concentration Source: GO_Central
Positive regulation of fat cell differentiation Source: UniProtKB
Positive regulation of macrophage chemotaxis Source: DFLAT
Regulation of calcium-mediated signaling Source: UniProtKB
Skeletal system development Source: ProtInc

Cellular Location

Cell membrane

Topology

Extracellular: 1-41
Helical: 42-64
Cytoplasmic: 65-75
Helical: 76-97
Extracellular: 98-114
Helical: 115-135
Cytoplasmic: 136-154
Helical: 155-176
Extracellular: 177-224
Helical: 225-245
Cytoplasmic: 246-261
Helical: 262-282
Extracellular: 283-300
Helical: 301-320
Cytoplasmic: 321-373

Fischer, T. F., Schoeder, C. T., Zellmann, T., Stichel, J., Meiler, J., & Beck-Sickinger, A. G. (2021). Cyclic analogues of the chemerin C-terminus mimic a loop conformation essential for activating the chemokine-like receptor 1. Journal of Medicinal Chemistry, 64(6), 3048-3058.

Fischer, T. F., Czerniak, A. S., Weiß, T., Zellmann, T., Zielke, L., Els-Heindl, S., & Beck-Sickinger, A. G. (2021). Cyclic derivatives of the chemerin C-terminus as metabolically stable agonists at the chemokine-like receptor 1 for cancer treatment. Cancers, 13(15), 3788.

Omori, A., Goshima, M., Kakuda, C., Kodama, T., Otani, K., Okada, M., & Yamawaki, H. (2020). Chemerin-9-induced contraction was enhanced through the upregulation of smooth muscle chemokine-like receptor 1 in isolated pulmonary artery of pulmonary arterial hypertensive rats. Pflügers Archiv-European Journal of Physiology, 472(3), 335-342.

Kumar, J. D., Aolymat, I., Tiszlavicz, L., Reisz, Z., Garalla, H. M., Beynon, R., ... & Varro, A. (2019). Chemerin acts via CMKLR1 and GPR1 to stimulate migration and invasion of gastric cancer cells: putative role of decreased TIMP-1 and TIMP-2. Oncotarget, 10(2), 98.

Yao, J., Li, Z., Fu, Y., Wu, R., Wang, Y., Liu, C., ... & Zhang, H. (2019). Involvement of obesity-associated upregulation of chemerin/chemokine-like receptor 1 in oxidative stress and apoptosis in ovaries and granulosa cells. Biochemical and biophysical research communications, 510(3), 449-455.

Zhao, H., Yan, D., Xiang, L., Huang, C., Li, J., Yu, X., ... & Zhang, J. V. (2019). Chemokine-like receptor 1 deficiency leads to lower bone mass in male mice. Cellular and Molecular Life Sciences, 76(2), 355-367.

Dranse, H. J., Zheng, A., Comeau, A. M., Langille, M. G., Zabel, B. A., & Sinal, C. J. (2018). The impact of chemerin or chemokine-like receptor 1 loss on the mouse gut microbiome. PeerJ, 6, e5494.

Yang, X., Yao, J., Wei, Q., Ye, J., Yin, X., Quan, X., ... & Xing, H. (2018). Role of chemerin/CMKLR1 in the maintenance of early pregnancy. Frontiers of medicine, 12(5), 525-532.

Perumalsamy, S., Ain Aqilah Mohd Zin, N., Teguh Widodo, R., Azman Wan Ahmad, W., Vethakkan, R. D., & Zaman Huri, H. (2017). Chemokine like receptor-1 (CMKLR-1) receptor: a potential therapeutic target in management of chemerin induced type 2 diabetes mellitus and cancer. Current pharmaceutical design, 23(25), 3689-3698.

Muruganandan, S., Govindarajan, R., McMullen, N. M., & Sinal, C. J. (2017). Chemokine-like receptor 1 is a novel Wnt target gene that regulates mesenchymal stem cell differentiation. Stem cells, 35(3), 711-724.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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