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Mouse Anti-COMMD1 Recombinant Antibody (3B3) (V2LY-1206-LY719)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat, Monkey
Clone
3B3
Antibody Isotype
IgG1
Application
WB, IHC, FC

Basic Information

Immunogen
Full length human recombinant protein of human COMMD1 produced in HEK293T cell.
Host Species
Mouse
Specificity
Human, Mouse, Rat, Monkey
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:2,000
IHC1:150
FC1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.3, 1% BSA, 50% Glycerol
Preservative
0.02% sodium azide
Concentration
1 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Copper Metabolism Domain Containing 1
Entrez Gene ID
Human150684
Mouse17846
Rat289831
UniProt ID
HumanQ8N668
MouseQ8K4M5
RatF7DLW7
Function
Proposed scaffold protein that is implicated in diverse physiological processes and whose function may be in part linked to its ability to regulate ubiquitination of specific cellular proteins. Can modulate activity of cullin-RING E3 ubiquitin ligase (CRL) complexes by displacing CAND1; in vitro promotes CRL E3 activity and dissociates CAND1 from CUL1 and CUL2 (PubMed:21778237).

Promotes ubiquitination of NF-kappa-B subunit RELA and its subsequent proteasomal degradation. Down-regulates NF-kappa-B activity (PubMed:15799966, PubMed:17183367, PubMed:20048074).

Involved in the regulation of membrane expression and ubiquitination of SLC12A2 (PubMed:23515529).

Modulates Na+ transport in epithelial cells by regulation of apical cell surface expression of amiloride-sensitive sodium channel (ENaC) subunits and by promoting their ubiquitination presumably involving NEDD4L. Promotes the localization of SCNN1D to recycling endosomes (PubMed:14645214, PubMed:20237237, PubMed:21741370).

Promotes CFTR cell surface expression through regulation of its ubiquitination (PubMed:21483833).

Down-regulates SOD1 activity by interfering with its homodimerization (PubMed:20595380).

Plays a role in copper ion homeostasis. Involved in copper-dependent ATP7A trafficking between the trans-Golgi network and vesicles in the cell periphery; the function is proposed to depend on its association within the CCC complex and cooperation with the WASH complex on early endosomes (PubMed:25355947).

Can bind one copper ion per monomer (PubMed:17309234).

May function to facilitate biliary copper excretion within hepatocytes. Binds to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) (PubMed:18940794).

Involved in the regulation of HIF1A-mediated transcription; competes with ARNT/Hif-1-beta for binding to HIF1A resulting in decreased DNA binding and impaired transcriptional activation by HIF-1 (PubMed:20458141).

Negatively regulates neuroblastoma G1/S phase cell cycle progression and cell proliferation by stimulating ubiquitination of NF-kappa-B subunit RELA and NF-kappa-B degradation in a FAM107A- and actin-dependent manner (PubMed:28604741).
Biological Process
Copper ion homeostasis Source: UniProtKB
Golgi to plasma membrane transport Source: UniProtKB
Negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
Negative regulation of protein localization to cell surface Source: UniProtKB
Negative regulation of sodium ion transmembrane transport Source: UniProtKB
Nucleotide-excision repair Source: CACAO
Plasma membrane to endosome transport Source: UniProtKB
Positive regulation of protein ubiquitination Source: UniProtKB
Post-translational protein modification Source: Reactome
Protein transport Source: UniProtKB-KW
Protein ubiquitination Source: Reactome
Regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
Cellular Location
Endosome membrane; Early endosome; Recycling endosome; Nucleus; Cytoplasm; Cytoplasmic vesicle. Shuttles between nucleus and cytosol. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins.
PTM
Acetylated by EP300 ina stimuli-specific manner; protecting it from XIAP-mediated proteasomal degradation and required for interaction with RElA in response to stress.
Ubiquitinated; undergoes both 'Lys-63'- and 'Lys-48'-linked polyubiquitination. Ubiquitinated by XIAP, leading to its proteasomal degradation.
More Infomation

Corbee, R. J., & Penning, L. C. (2021). COMMD1 Exemplifies the Power of Inbred Dogs to Dissect Genetic Causes of Rare Copper-Related Disorders. Animals, 11(3), 601.

Weiskirchen, R., & Penning, L. C. (2021). COMMD1, a multi-potent intracellular protein involved in copper homeostasis, protein trafficking, inflammation, and cancer. Journal of Trace Elements in Medicine and Biology, 65, 126712.

Li, C., Wang, T., Xiao, Y., Li, K., Meng, X., & James Kang, Y. (2021). COMMD1 upregulation is involved in copper efflux from ischemic hearts. Experimental Biology and Medicine, 246(5), 607-616.

Jiang, Z., Chen, W., Zhou, J., Peng, Q., Zheng, H., Yuan, Y., ... & Liu, X. (2019). Identification of COMMD1 as a novel lamin A binding partner. Molecular medicine reports, 20(2), 1790-1796.

Stewart, D. J., Short, K. K., Maniaci, B. N., & Burkhead, J. L. (2019). COMMD1 and PtdIns (4, 5) P2 interaction maintain ATP7B copper transporter trafficking fidelity in HepG2 cells. Journal of cell science, 132(19), jcs231753.

Jiang, Z., Yuan, Y., Zheng, H., Cui, H., Sun, X., Zhao, W., & Liu, X. (2019). COMMD1 regulates cell proliferation and cell cycle progression by modulating p21 Cip1 levels. Bioscience, Biotechnology, and Biochemistry, 83(5), 845-850.

Li, K., Li, C., Xiao, Y., Wang, T., & James Kang, Y. (2018). Featured Article: The loss of copper is associated with the increase in copper metabolism MURR domain 1 in ischemic hearts of mice. Experimental Biology and Medicine, 243(9), 780-785.

Riera‐Romo, M. (2018). COMMD1: a multifunctional regulatory protein. Journal of Cellular Biochemistry, 119(1), 34-51.

Fedoseienko, A., Wijers, M., Wolters, J. C., Dekker, D., Smit, M., Huijkman, N., ... & Van De Sluis, B. (2018). The COMMD family regulates plasma LDL levels and attenuates atherosclerosis through stabilizing the CCC complex in endosomal LDLR trafficking. Circulation research, 122(12), 1648-1660.

Mu, P., Akashi, T., Lu, F., Kishida, S., & Kadomatsu, K. (2017). A novel nuclear complex of DRR1, F-actin and COMMD1 involved in NF-κB degradation and cell growth suppression in neuroblastoma. Oncogene, 36(41), 5745-5756.

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For research use only. Not intended for any clinical use.

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