Mouse Anti-CYSLTR2 Recombinant Antibody (CBLC209-LY) (V2LY-0125-LY1018)

Name: Mouse Anti-CYSLTR2 Recombinant Antibody (CBLC209-LY)
SKU: V2LY-0125-LY1018
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBLC209-LY

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 213-229 within an N-terminal cytoplasmic domain of CysLT2 Receptor of mouse.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgM, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cysteinyl Leukotriene Receptor 2

Entrez Gene ID

Mouse	70086
Rat	170926

UniProt ID

Mouse	Q920A1
Rat	Q924T9

Function

Receptor for cysteinyl leukotrienes. The response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Stimulation by BAY u9773, a partial agonist, induces specific contractions of pulmonary veins and might also have an indirect role in the relaxation of the pulmonary vascular endothelium. The rank order of affinities for the leukotrienes is LTC4 = LTD4>> LTE4.

Biological Process

Cellular response to glucagon stimulus Source: Reactome
G protein-coupled receptor signaling pathway Source: Reactome
Immune response Source: UniProtKB
Negative regulation of inflammatory response to antigenic stimulus Source: Reactome
Neuropeptide signaling pathway Source: GO_Central

Cellular Location

Cell membrane

Topology

Extracellular: 1-42
Helical: 43-63
Cytoplasmic: 64-72
Helical: 73-93
Extracellular: 94-123
Helical: 124-144
Cytoplasmic: 145-153
Helical: 154-174
Extracellular: 175-204
Helical: 205-225
Cytoplasmic: 226-245
Helical: 246-266
Extracellular: 267-286
Helical: 287-307
Cytoplasmic: 308-346

Ceraudo, E., Horioka, M., Mattheisen, J. M., Hitchman, T. D., Moore, A. R., Kazmi, M. A., ... & Huber, T. (2021). Direct evidence that the GPCR CysLTR2 mutant causative of uveal melanoma is constitutively active with highly biased signaling. Journal of Biological Chemistry, 296.

Trinh, H. K. T., Suh, D. H., Nguyen, T. V. T., Choi, Y., Park, H. S., & Shin, Y. S. (2019). Characterization of cysteinyl leukotriene-related receptors and their interactions in a mouse model of asthma. Prostaglandins, Leukotrienes and Essential Fatty Acids, 141, 17-23.

Duah, E., Teegala, L. R., Kondeti, V., Adapala, R. K., Keshamouni, V. G., Kanaoka, Y., ... & Paruchuri, S. (2019). Cysteinyl leukotriene 2 receptor promotes endothelial permeability, tumor angiogenesis, and metastasis. Proceedings of the National Academy of Sciences, 116(1), 199-204.

Slater, K., Hoo, P. S., Buckley, A. M., Piulats, J. M., Villanueva, A., Portela, A., & Kennedy, B. N. (2018). Evaluation of oncogenic cysteinyl leukotriene receptor 2 as a therapeutic target for uveal melanoma. Cancer and Metastasis Reviews, 37(2), 335-345.

Liu, T., Barrett, N. A., Kanaoka, Y., Yoshimoto, E., Garofalo, D., Cirka, H., ... & Boyce, J. A. (2018). Type 2 cysteinyl leukotriene receptors drive IL-33–dependent type 2 immunopathology and aspirin sensitivity. The Journal of Immunology, 200(3), 915-927.

Möller, I., Murali, R., Müller, H., Wiesner, T., Jackett, L. A., Scholz, S. L., ... & Griewank, K. G. (2017). Activating cysteinyl leukotriene receptor 2 (CYSLTR2) mutations in blue nevi. Modern Pathology, 30(3), 350-356.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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