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Mouse Anti-DDX4 Recombinant Antibody (CBT3566) (V2LY-0625-LY2371)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBT3566
Antibody Isotype
IgG1
Application
IHC, ICC, FC

Basic Information

Immunogen
Purified recombinant fragment of human DDX4 expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
IHC-P1:200-1:1,000
ICC1:200-1:1,000
FC1:200-1:400
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Preservative
Sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
DEAD-Box Helicase 4
Entrez Gene ID
54514
UniProt ID
Q9NQI0
Alternative Names
DEAD-Bovinex Helicase 4; DEAD/H (Asp-Glu-Ala-Asp/His) Bovinex Polypeptide 4; DEAD (Asp-Glu-Ala-Asp) Bovinex Polypeptide 4; DEAD Bovinex Protein 4; Vasa Homolog; VASA; Probable ATP-Dependent RNA Helicase DDX4; EC 3.6.4.13; EC 3.6.1;
Function
ATP-dependent RNA helicase required during spermatogenesis (PubMed:10920202, PubMed:21034600).

Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity (By similarity).

Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (By similarity).

Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle (By similarity).

Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs (By similarity).
Biological Process
Cell differentiation Source: GO_Central
DNA methylation involved in gamete generation Source: UniProtKB
Flagellated sperm motility Source: UniProtKB
Gamete generation Source: GO_Central
Gene silencing by RNA Source: UniProtKB-KW
Male meiosis I Source: UniProtKB
Male meiotic nuclear division Source: UniProtKB
Multicellular organism development Source: UniProtKB-KW
Negative regulation of transposition Source: UniProtKB
PiRNA biosynthetic process Source: UniProtKB
PiRNA metabolic process Source: UniProtKB
Spermatogenesis Source: UniProtKB
Cellular Location
Cytoplasm; Perinuclear region. Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis.
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For research use only. Not intended for any clinical use.

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