Mouse Anti-DIAPH3 Recombinant Antibody (4D5) (V2LY-0425-LY323)

Name: Mouse Anti-DIAPH3 Recombinant Antibody (4D5)
SKU: V2LY-0425-LY323
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-DIAPH3 Recombinant Antibody (4D5) (V2LY-0425-LY323-1) in IF

Actin isolated from human platelets nucleated on glass by GST-DIAPH3-CT, fixed, and stained with β- and γ-actin-specific antibodies. Actin isolated from chicken gizzard nucleated on glass by GST-DIAPH3-CT, fixed, and stained with β- and γ-actin-specific antibodies. ...View More

Chen, A., Arora, P. D., McCulloch, C. A., & Wilde, A. (2017). Cytokinesis requires localized β-actin filament production by an actin isoform specific nucleator. Nature Communications, 8(1), 1530.

Mouse Anti-DIAPH3 Recombinant Antibody (4D5) (V2LY-0425-LY323-2) in Immunoblot

Immunoblots and quantification to determine the amount of GST-DIAPH3-CT and MBP-DIAPH3-NT bound to amylose beads in the presence of increasing concentrations of GST, GST-RhoA, and 6× His-anillin(1-151 aa). Values normalized to the amount of MBP-DIAPH3-NT immobilized on beads. Each binding assay was repeated at least three times. Error bars indicate±s.e.m. *p=0.014, **p=0.0009 compared to GST control, Student's t test. n=3. ...View More

Chen, A., Arora, P. D., McCulloch, C. A., & Wilde, A. (2017). Cytokinesis requires localized β-actin filament production by an actin isoform specific nucleator. Nature Communications, 8(1), 1530.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4D5

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 632-729 of human DIAPH3.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.1 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

diaphanous homolog 3 (Drosophila)

Entrez Gene ID

81624

UniProt ID

Q9NSV4

Function

Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers. Required for cytokinesis, stress fiber formation and transcriptional activation of the serum response factor. Binds to GTP-bound form of Rho and to profilin: acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity.

Biological Process

Actin cytoskeleton organization Source: UniProtKB
Actin filament polymerization Source: UniProtKB
Cytoskeleton organization Source: UniProtKB

Cellular Location

Nucleus; Cytoplasm. During mitosis, co-localizes with the actin-rich cleavage furrow and with the microtubule-rich central spindle during cytokinesis (PubMed:18755006, PubMed:19457867). Shuttles between the cytoplasm and the nucleus (By similarity).

Involvement in disease

Auditory neuropathy, autosomal dominant, 1 (AUNA1):
A form of sensorineural hearing loss with absent or severely abnormal auditory brainstem response, in the presence of normal cochlear outer hair cell function and normal otoacoustic emissions. Auditory neuropathies result from a lesion in the area including the inner hair cells, connections between the inner hair cells and the cochlear branch of the auditory nerve, the auditory nerve itself and auditory pathways of the brainstem.

PTM

Ubiquitinated.

Zhang, Z., Dai, F., Luo, F., Wu, W., Zhang, S., Zhou, R., ... & Song, L. (2021). Diaphanous related formin 3 knockdown suppresses cell proliferation and metastasis of osteosarcoma cells. Discover Oncology, 12(1), 1-12.

Lau, E. O. C., Damiani, D., Chehade, G., Ruiz-Reig, N., Saade, R., Jossin, Y., ... & Tissir, F. (2021). DIAPH3 deficiency links microtubules to mitotic errors, defective neurogenesis, and brain dysfunction. Elife, 10, e61974.

Wan, L., Zhu, J., & Wu, Q. (2021). Knockdown of DIAPH3 Inhibits the Proliferation of Cervical Cancer Cells through Inactivating mTOR Signaling Pathway. Journal of Oncology, 2021.

Xiang, G., Weiwei, H., Erji, G., & Haitao, M. (2019). DIAPH3 promotes the tumorigenesis of lung adenocarcinoma. Experimental cell research, 385(1), 111662.

Dong, L., Li, Z., Xue, L., Li, G., Zhang, C., Cai, Z., ... & Guo, R. (2018). DIAPH3 promoted the growth, migration and metastasis of hepatocellular carcinoma cells by activating beta-catenin/TCF signaling. Molecular and cellular biochemistry, 438(1), 183-190.

Jiang, J. (2017). Diaphanous-related formin-3 overexpression inhibits the migration and invasion of triple-negative breast cancer by inhibiting RhoA-GTP expression. Biomedicine & Pharmacotherapy, 94, 439-445.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-DIAPH3 Recombinant Antibody (4D5) (CAT#: CBMAB-D0890-YC)

Related Products

Mouse Anti-DIAPH3 Recombinant Antibody (4D5) (CAT#: CBMAB-D0890-YC)

Isotype control

For research use only. Not intended for any clinical use.

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