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Mouse Anti-DIAPH3 Recombinant Antibody (4D5) (CBMAB-D0890-YC)

Provided herein is a Mouse monoclonal antibody, which binds to Diaphanous Related Formin 3 (DIAPH3). The antibody can be used for immunoassay techniques, such as ELISA, IF, WB.
See all DIAPH3 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
4D5
Antibody Isotype
IgG2a, κ
Application
ELISA, IF, WB

Basic Information

Immunogen
DIAPH3 (NP_112194, 632 a.a. ~ 729 a.a) partial recombinant protein with GST tag. The immunogen sequence: PDILNFVDDL EPLDKASKVS VETLEKNLRQ MGRQLQQLEK ELETFPPPED LHDKFVTKMS RFVISAKEQY ETLSKLHENM EKLYQSIIGY YAIDVKKV
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 632-729

Target

Full Name
diaphanous homolog 3 (Drosophila)
Introduction
DIAPH3 belongs to the diaphanous subfamily of the formin family. Members of this family are involved in actin remodeling and regulate cell movement and adhesion. Mutations in this gene are associated with autosomal dominant auditory neuropathy 1.
Entrez Gene ID
81624
UniProt ID
Q9NSV4
Alternative Names
Diaphanous Related Formin 3; Diap3; MDia2; DRF3; Auditory Neuropathy, Autosomal Dominant 1; Diaphanous (Drosophila, Homolog) 3; Diaphanous Homolog 3 (Drosophila); Protein Diaphanous Homolog 3;
Function
Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers. Required for cytokinesis, stress fiber formation and transcriptional activation of the serum response factor. Binds to GTP-bound form of Rho and to profilin: acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity.
Biological Process
Actin cytoskeleton organization Source: UniProtKB
Actin filament polymerization Source: UniProtKB
Cytoskeleton organization Source: UniProtKB
Cellular Location
Nucleus; Cytoplasm. During mitosis, co-localizes with the actin-rich cleavage furrow and with the microtubule-rich central spindle during cytokinesis (PubMed:18755006, PubMed:19457867). Shuttles between the cytoplasm and the nucleus (By similarity).
Involvement in disease
Auditory neuropathy, autosomal dominant, 1 (AUNA1):
A form of sensorineural hearing loss with absent or severely abnormal auditory brainstem response, in the presence of normal cochlear outer hair cell function and normal otoacoustic emissions. Auditory neuropathies result from a lesion in the area including the inner hair cells, connections between the inner hair cells and the cochlear branch of the auditory nerve, the auditory nerve itself and auditory pathways of the brainstem.
PTM
Ubiquitinated.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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