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Mouse Anti-DLC1 Recombinant Antibody (D1009) (CBMAB-D1009-YC)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
D1009
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA, IHC-P

Basic Information

Immunogen
Amino acids 111-370 mapping within an internal region of human DLC-1.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
DLC1
Introduction
DLC1 is a GTPase-activating protein (GAP) that is a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-binding proteins. GAP family proteins participate in signaling pathways that regulate cell processes involved in cytoskeletal changes. This gene functions as a tumor suppressor gene in a number of common cancers, including prostate, lung, colorectal, and breast cancers. Multiple transcript variants due to alternative promoters and alternative splicing have been found for this gene.
Entrez Gene ID
10395
UniProt ID
Q96QB1
Alternative Names
DLC1 Rho GTPase Activating Protein; StAR-Related Lipid Transfer (START) Domain Containing 12; Rho-Type GTPase-Activating Protein 7; START Domain-Containing Protein 12; Deleted In Liver Cancer 1 Protein; ARHGAP7; STARD12; StAR-Related Lipid Transfer Protein 12; Deleted In Liver Cancer 1 Variant 2; Deleted In Liver Cancer Variant 4;
Function
Functions as a GTPase-activating protein for the small GTPases RHOA, RHOB, RHOC and CDC42, terminating their downstream signaling. This induces morphological changes and detachment through cytoskeletal reorganization, playing a critical role in biological processes such as cell migration and proliferation. Also functions in vivo as an activator of the phospholipase PLCD1. Active DLC1 increases cell migration velocity but reduces directionality.
Biological Process
Actin cytoskeleton organization Source: UniProtKB
Activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
Apoptotic process Source: UniProtKB
Focal adhesion assembly Source: UniProtKB
Forebrain development Source: UniProtKB
Heart morphogenesis Source: UniProtKB
Hindbrain morphogenesis Source: UniProtKB
Negative regulation of cell migration Source: UniProtKB
Negative regulation of cell population proliferation Source: UniProtKB
Negative regulation of focal adhesion assembly Source: UniProtKB
Negative regulation of Rho protein signal transduction Source: UniProtKB
Negative regulation of stress fiber assembly Source: UniProtKB
Neural tube closure Source: UniProtKB
Positive regulation of execution phase of apoptosis Source: UniProtKB
Positive regulation of protein dephosphorylation Source: UniProtKB
Regulation of actin cytoskeleton organization Source: UniProtKB
Regulation of cell shape Source: UniProtKB
Regulation of Rho protein signal transduction Source: GO_Central
Regulation of small GTPase mediated signal transduction Source: Reactome
Signal transduction Source: InterPro
Cellular Location
Cytoplasm; Focal adhesion; Membrane. Colocalizes with EF1A1 at actin-rich regions in the cell periphery.
More Infomation

Frey, Y., Franz-Wachtel, M., Macek, B., & Olayioye, M. A. (2022). Proteasomal turnover of the RhoGAP tumor suppressor DLC1 is regulated by HECTD1 and USP7. Scientific reports, 12(1), 1-10.

Ren, G., & Li, G. (2021). Tumor suppressor gene DLC1: Its modifications, interactive molecules, and potential prospects for clinical cancer application. International Journal of Biological Macromolecules, 182, 264-275.

Zhang, Y., & Li, G. (2020). A tumor suppressor DLC1: The functions and signal pathways. Journal of Cellular Physiology, 235(6), 4999-5007.

Yang, X., Hu, F., Liu, J. A., Yu, S., Cheung, M. P. L., Liu, X., ... & Cheung, M. (2020). Nuclear DLC1 exerts oncogenic function through association with FOXK1 for cooperative activation of MMP9 expression in melanoma. Oncogene, 39(20), 4061-4076.

Wang, D., Qian, X., Sanchez-Solana, B., Tripathi, B. K., Durkin, M. E., & Lowy, D. R. (2020). Cancer-associated point mutations in the DLC1 tumor suppressor and other Rho-GAPs occur frequently and are associated with decreased function. Cancer research, 80(17), 3568-3579.

Tripathi, B. K., & Lowy, D. R. (2017). DLC1: A tumor suppressor that regulates Rho signaling. Oncotarget, 8(17), 27674.

Tripathi, B. K., Grant, T., Qian, X., Zhou, M., Mertins, P., Wang, D., ... & Lowy, D. R. (2017). Receptor tyrosine kinase activation of RhoA is mediated by AKT phosphorylation of DLC1. Journal of Cell Biology, 216(12), 4255-4270.

Shih, Y. P., Yuan, S. Y., & Lo, S. H. (2017). Down-regulation of DLC1 in endothelial cells compromises the angiogenesis process. Cancer letters, 398, 46-51.

Sim, C. K., Kim, S. Y., Brunmeir, R., Zhang, Q., Li, H., Dharmasegaran, D., ... & Xu, F. (2017). Regulation of white and brown adipocyte differentiation by RhoGAP DLC1. PLoS One, 12(3), e0174761.

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For research use only. Not intended for any clinical use.

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