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Mouse Anti-DMC1 Recombinant Antibody (2H12/4) (CBMAB-D1174-YC)

Provided herein is a Mouse monoclonal antibody, which binds to DNA Meiotic Recombinase 1 (DMC1). The antibody can be used for immunoassay techniques, such as WB, IP, IHC-P, FC, ICC, IF.
See all DMC1 antibodies
Published Data
Fig.1 Nuclei of Spo11YF/YFzygotene spermatocytes were divided in four subgroups depending on their positivity for the pseudo XY body and for foci of one of the three DNA repair proteins DMC1 (n = 227) as follows: 1) with pseudo XY body and with foci, 2) with pseudo XY body and without foci, 3) without pseudo XY body and with foci, 4) without pseudo XY body and without foci. Spermatocyte nuclei were immunostained with anti-SYCP3 (red), anti-γH2AX (blue), and one of the following antibodies: anti-DMC1 (green, middle panel).
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Fig.2 Double immunostaining of irradiated (as in A) leptotene nucleus with anti-DMC1 (red) and anti-RAD18 (green). On the right, an enlarged region is shown with separate red and green images, to better visualize foci that do and do not colocalize.
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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat, Cattle
Clone
2H12/4
Antibody Isotype
IgG2a
Application
WB, IP, IHC-P, FC, ICC, IF

Basic Information

Specificity
Human, Mouse, Rat, Cattle
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
DNA Meiotic Recombinase 1
Introduction
DMC1 belongs to the superfamily of recombinases (also called DNA strand-exchange proteins). Recombinases are important for repairing double-strand DNA breaks during mitosis and meiosis. This protein, which is evolutionarily conserved, is reported to be essential for meiotic homologous recombination and may thus play an important role in generating diversity of genetic information.
Entrez Gene ID
Human11144
Mouse13404
Rat362960
Cattle618398
UniProt ID
HumanQ14565
MouseQ61880
RatD3ZJ85
CattleF1N1D2
Alternative Names
DNA Meiotic Recombinase 1; DMC1H; LIM15; DMC1 (Dosage Suppressor Of Mck1, Yeast Homolog) Meiosis-Specific Homologous Recombination; DMC1 Dosage Suppressor Of Mck1 Homolog, Meiosis-Specific Homologous Recombination (Yeast); DMC1 Dosage Suppressor Of Mck1 Homolog, Meiosis-Specific Homologous Recombination; Meiotic Recombination Protein DMC1/LIM15 Homolog; Disrupted Meiotic CDNA1, Yeast, Homolog Of; DJ199H16.1;
Function
Participates in meiotic recombination, specifically in homologous strand assimilation, which is required for the resolution of meiotic double-strand breaks.
Biological Process
Chromosome organization involved in meiotic cell cycle Source: GO_Central
DNA recombinase assembly Source: GO_Central
Female gamete generation Source: ProtInc
Homologous chromosome pairing at meiosis Source: Ensembl
Male meiosis I Source: Ensembl
Meiotic cell cycle Source: ProtInc
Mitotic recombination Source: GO_Central
Oocyte maturation Source: Ensembl
Ovarian follicle development Source: Ensembl
Reciprocal meiotic recombination Source: UniProtKB
Spermatid development Source: Ensembl
Spermatogenesis Source: ProtInc
Strand invasion Source: GO_Central
Cellular Location
Nucleus; Chromosome

Takemoto, K., Tani, N., Takada-Horisawa, Y., Fujimura, S., Tanno, N., Yamane, M., ... & Ishiguro, K. I. (2020). Meiosis-specific C19orf57/4930432K21Rik/BRME1 modulates localization of RAD51 and DMC1 to DSBs in mouse meiotic recombination. Cell reports, 31(8), 107686.

Lan, W. H., Lin, S. Y., Kao, C. Y., Chang, W. H., Yeh, H. Y., Chang, H. Y., ... & Li, H. W. (2020). Rad51 facilitates filament assembly of meiosis-specific Dmc1 recombinase. Proceedings of the National Academy of Sciences, 117(21), 11257-11264.

Slotman, J. A., Paul, M. W., Carofiglio, F., de Gruiter, H. M., Vergroesen, T., Koornneef, L., ... & Baarends, W. M. (2020). Super-resolution imaging of RAD51 and DMC1 in DNA repair foci reveals dynamic distribution patterns in meiotic prophase. PLoS genetics, 16(6), e1008595.

Hinch, A. G., Becker, P. W., Li, T., Moralli, D., Zhang, G., Bycroft, C., ... & Donnelly, P. (2020). The configuration of RPA, RAD51, and DMC1 binding in meiosis reveals the nature of critical recombination intermediates. Molecular cell, 79(4), 689-701.

Steinfeld, J. B., Beláň, O., Kwon, Y., Terakawa, T., Al-Zain, A., Smith, M. J., ... & Greene, E. C. (2019). Defining the influence of Rad51 and Dmc1 lineage-specific amino acids on genetic recombination. Genes & development, 33(17-18), 1191-1207.

Chan, Y. L., Zhang, A., Weissman, B. P., & Bishop, D. K. (2019). RPA resolves conflicting activities of accessory proteins during reconstitution of Dmc1-mediated meiotic recombination. Nucleic acids research, 47(2), 747-761.

Crickard, J. B., Kaniecki, K., Kwon, Y., Sung, P., & Greene, E. C. (2018). Spontaneous self-segregation of Rad51 and Dmc1 DNA recombinases within mixed recombinase filaments. Journal of Biological Chemistry, 293(11), 4191-4200.

Crickard, J. B., Kaniecki, K., Kwon, Y., Sung, P., & Greene, E. C. (2018). Meiosis-specific recombinase Dmc1 is a potent inhibitor of the Srs2 antirecombinase. Proceedings of the National Academy of Sciences, 115(43), E10041-E10048.

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For research use only. Not intended for any clinical use.

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