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Mouse Anti-DNAJB1 Recombinant Antibody (D1281) (V2LY-0425-LY624)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
D1281
Antibody Isotype
IgG1
Application
WB, IHC, IF, IP, ELISA

Basic Information

Immunogen
Recombinant Human Protein HSP40.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:2,000
IF(ICC)1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Glycerol & PBS
Preservative
Sodium Azide
Concentration
1 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
DnaJ Heat Shock Protein Family (Hsp40) Member B1
Entrez Gene ID
Human3337
Mouse81489
Rat361384
UniProt ID
HumanP25685
MouseQ9QYJ3
RatB0K030
Function
Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response (PubMed:9499401).

Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro) (PubMed:24318877).
Biological Process
Chaperone cofactor-dependent protein refolding Source: UniProtKB
Forebrain development Source: Ensembl
Negative regulation of inclusion body assembly Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: GO_Central
Negative regulation of transcription from RNA polymerase II promoter in response to stress Source: UniProtKB
Positive regulation of ATPase activity Source: BHF-UCL
Regulation of cellular response to heat Source: Reactome
Response to unfolded protein Source: ProtInc
Cellular Location
Nucleus; Nucleolus; Cytoplasm. Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.
More Infomation

Zhang, Z., Zhang, X., Wu, X., Zhang, Y., Lu, J., & Li, D. (2022). Sirt1 Attenuates Astrocyte Activation Via Modulating Dnajb1 and Chaperone-Mediated Autophagy after Closed Head Injury. Cerebral Cortex.

Kim, S. S., Kycia, I., Karski, M., Ma, R. K., Bordt, E. A., Kwan, J., ... & Vakili, K. (2022). DNAJB1-PRKACA in HEK293T cells induces LINC00473 overexpression that depends on PKA signaling. PloS one, 17(2), e0263829.

Vyas, M., Hechtman, J. F., Zhang, Y., Benayed, R., Yavas, A., Askan, G., ... & Basturk, O. (2020). DNAJB1-PRKACA fusions occur in oncocytic pancreatic and biliary neoplasms and are not specific for fibrolamellar hepatocellular carcinoma. Modern Pathology, 33(4), 648-656.

Lu, T. W., Aoto, P. C., Weng, J. H., Nielsen, C., Cash, J. N., Hall, J., ... & Taylor, S. S. (2020). Structural analyses of the PKA RIIβ holoenzyme containing the oncogenic DnaJB1-PKAc fusion protein reveal protomer asymmetry and fusion-induced allosteric perturbations in fibrolamellar hepatocellular carcinoma. PLoS biology, 18(12), e3001018.

Tomasini, M. D., Wang, Y., Karamafrooz, A., Li, G., Beuming, T., Gao, J., ... & Simon, S. M. (2018). Conformational landscape of the PRKACA-DNAJB1 chimeric kinase, the driver for fibrolamellar hepatocellular carcinoma. Scientific reports, 8(1), 1-11.

Silva, G., Marins, M., Chaichanasak, N., Yoon, Y., Fachin, A. L., Pinhanelli, V. C., ... & Baek, S. J. (2018). Trans-chalcone increases p53 activity via DNAJB1/HSP40 induction and CRM1 inhibition. PLoS One, 13(8), e0202263.

Graham, R. P., Lackner, C., Terracciano, L., González‐Cantú, Y., Maleszewski, J. J., Greipp, P. T., ... & Torbenson, M. S. (2018). Fibrolamellar carcinoma in the Carney complex: PRKAR1A loss instead of the classic DNAJB1‐PRKACA fusion. Hepatology, 68(4), 1441-1447.

Engelholm, L. H., Riaz, A., Serra, D., Dagnæs-Hansen, F., Johansen, J. V., Santoni-Rugiu, E., ... & Frödin, M. (2017). CRISPR/Cas9 engineering of adult mouse liver demonstrates that the Dnajb1–Prkaca gene fusion is sufficient to induce tumors resembling fibrolamellar hepatocellular carcinoma. Gastroenterology, 153(6), 1662-1673.

Kastenhuber, E. R., Lalazar, G., Houlihan, S. L., Tschaharganeh, D. F., Baslan, T., Chen, C. C., ... & Lowe, S. W. (2017). DNAJB1–PRKACA fusion kinase interacts with β-catenin and the liver regenerative response to drive fibrolamellar hepatocellular carcinoma. Proceedings of the National Academy of Sciences, 114(50), 13076-13084.

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For research use only. Not intended for any clinical use.

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