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Mouse Anti-DNMT3L Recombinant Antibody (D1438) (V2LY-0425-LY737)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
D1438
Antibody Isotype
IgG1
Application
ELISA, WB, FC

Basic Information

Immunogen
Purified recombinant fragment of human DNMT3L (aa147-386) expressed in E. coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
ELISA1:10,000
FC1:200-1:400

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
Sodium Azide
Concentration
1 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
DNA Methyltransferase 3 Like
Entrez Gene ID
29947
UniProt ID
Q9UJW3
Function
Catalytically inactive regulatory factor of DNA methyltransferases that can either promote or inhibit DNA methylation depending on the context (By similarity).

Essential for the function of DNMT3A and DNMT3B: activates DNMT3A and DNMT3B by binding to their catalytic domain (PubMed:17687327).

Acts by accelerating the binding of DNA and S-adenosyl-L-methionine (AdoMet) to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases (PubMed:17687327).

Recognizes unmethylated histone H3 lysine 4 (H3K4me0) and induces de novo DNA methylation by recruitment or activation of DNMT3 (PubMed:17687327).

Plays a key role in embryonic stem cells and germ cells (By similarity).

In germ cells, required for the methylation of imprinted loci together with DNMT3A (By similarity).

In male germ cells, specifically required to methylate retrotransposons, preventing their mobilization (By similarity).

Plays a key role in embryonic stem cells (ESCs) by acting both as an positive and negative regulator of DNA methylation (By similarity).

While it promotes DNA methylation of housekeeping genes together with DNMT3A and DNMT3B, it also acts as an inhibitor of DNA methylation at the promoter of bivalent genes (By similarity).

Interacts with the EZH2 component of the PRC2/EED-EZH2 complex, preventing interaction of DNMT3A and DNMT3B with the PRC2/EED-EZH2 complex, leading to maintain low methylation levels at the promoters of bivalent genes (By similarity).

Promotes differentiation of ESCs into primordial germ cells by inhibiting DNA methylation at the promoter of RHOX5, thereby activating its expression (By similarity).
Biological Process
DNA methylation Source: UniProtKB
DNA methylation involved in gamete generation Source: UniProtKB
DNA methylation on cytosine Source: UniProtKB
Male meiosis I Source: UniProtKB
Negative regulation of DNA methylation Source: UniProtKB
Negative regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of DNA methylation Source: UniProtKB
Regulation of gene expression by genetic imprinting Source: UniProtKB
Spermatogenesis Source: UniProtKB
Stem cell differentiation Source: UniProtKB
Cellular Location
Nucleus
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For research use only. Not intended for any clinical use.

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