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Mouse Anti-DOCK4 Recombinant Antibody (1B3) (CBMAB-D1480-YC)

Provided herein is a Mouse monoclonal antibody, which binds to Dedicator Of Cytokinesis 4 (DOCK4). The antibody can be used for immunoassay techniques, such as WB.
See all DOCK4 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1B3
Antibody Isotype
IgG3, κ
Application
WB

Basic Information

Immunogen
DOCK4 (NP_055520, 1867 a.a. ~ 1967 a.a) partial recombinant protein with GST tag.
Specificity
Human
Antibody Isotype
IgG3, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
dedicator of cytokinesis 4
Introduction
DOCK4 is a member of the dedicator of cytokinesis (DOCK) family and encodes a protein with a DHR-1 (CZH-1) domain, a DHR-2 (CZH-2) domain and an SH3 domain. This membrane-associated, cytoplasmic protein functions as a guanine nucleotide exchange factor and is involved in regulation of adherens junctions between cells. Mutations in this gene have been associated with ovarian, prostate, glioma, and colorectal cancers. Alternatively spliced variants which encode different protein isoforms have been described, but only one has been fully characterized.
Entrez Gene ID
9732
UniProt ID
Q8N1I0
Alternative Names
Dedicator Of Cytokinesis 4; KIAA0716;
Function
Functions as a guanine nucleotide exchange factor (GEF) that promotes the exchange of GDP to GTP, converting inactive GDP-bound small GTPases into their active GTP-bound form (PubMed:12628187, PubMed:16464467).

Involved in regulation of adherens junction between cells (PubMed:12628187).

Plays a role in cell migration (PubMed:20679435).

Isoform 2:
Has a higher guanine nucleotide exchange factor activity compared to other isoforms.
Biological Process
Cell chemotaxis Source: UniProtKB
Negative regulation of vascular associated smooth muscle contraction Source: BHF-UCL
Positive regulation of vascular associated smooth muscle cell migration Source: BHF-UCL
Small GTPase mediated signal transduction Source: InterPro
Cellular Location
Cell membrane; Cytosol; Cell projection. Colocalizes with EPHA2, RHOG and CTTN/cortactin at the tip of protrusions in migrating cells.

Yazbeck, P., Cullere, X., Bennett, P., Yajnik, V., Wang, H., Kawada, K., ... & Mayadas, T. N. (2022). DOCK4 Regulation of Rho GTPases Mediates Pulmonary Vascular Barrier Function. Arteriosclerosis, Thrombosis, and Vascular Biology, 10-1161.

Huang, Y., Yang, Y., Zhao, Y., Guo, D., Chen, L., Shi, L., & Xu, G. (2022). DOCK4 regulates ghrelin production in gastric X/A-like cells. Journal of Endocrinological Investigation, 1-8.

Guo, D., Peng, Y., Wang, L., Sun, X., Wang, X., Liang, C., ... & Shi, L. (2021). Autism-like social deficit generated by Dock4 deficiency is rescued by restoration of Rac1 activity and NMDA receptor function. Molecular psychiatry, 26(5), 1505-1519.

Qin, T., Yang, J., Huang, D., Zhang, Z., Huang, Y., Chen, H., & Xu, G. (2021). DOCK4 stimulates MUC2 production through its effect on goblet cell differentiation. Journal of Cellular Physiology, 236(9), 6507-6519.

Yang, X., Guo, D., Li, K., & Shi, L. (2021). Altered postnatal developmental patterns of ultrasonic vocalizations in Dock4 knockout mice. Behavioural Brain Research, 406, 113232.

McNally, L., Zhou, Y., Robinson, J. F., Zhao, G., Chen, L. M., Chen, H., ... & Fisher, S. J. (2020). Up-regulated cytotrophoblast DOCK4 contributes to over-invasion in placenta accreta spectrum. Proceedings of the National Academy of Sciences, 117(27), 15852-15861.

Huang, L., Chambliss, K. L., Gao, X., Yuhanna, I. S., Behling-Kelly, E., Bergaya, S., ... & Shaul, P. W. (2019). SR-B1 drives endothelial cell LDL transcytosis via DOCK4 to promote atherosclerosis. Nature, 569(7757), 565-569.

Westbrook, J. A., Wood, S. L., Cairns, D. A., McMahon, K., Gahlaut, R., Thygesen, H., ... & Brown, J. E. (2019). Identification and validation of DOCK4 as a potential biomarker for risk of bone metastasis development in patients with early breast cancer. The Journal of pathology, 247(3), 381-391.

Sundaravel, S., Kuo, W. L., Jeong, J. J., Choudhary, G. S., Gordon-Mitchell, S., Liu, H., ... & Wickrema, A. (2019). Loss of function of DOCK4 in myelodysplastic syndromes stem cells is restored by inhibitors of DOCK4 signaling networks. Clinical Cancer Research, 25(18), 5638-5649.

Debruyne, D. N., Turchi, L., Burel-Vandenbos, F., Fareh, M., Almairac, F., Virolle, V., ... & Virolle, T. (2018). DOCK4 promotes loss of proliferation in glioblastoma progenitor cells through nuclear beta-catenin accumulation and subsequent miR-302-367 cluster expression. Oncogene, 37(2), 241-254.

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For research use only. Not intended for any clinical use.

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