Mouse Anti-DUSP16 Recombinant Antibody (3F3) (CBMAB-D1887-YC)

Name: Mouse Anti-DUSP16 Recombinant Antibody (3F3)
SKU: CBMAB-D1887-YC
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

3F3

Antibody Isotype

IgG2a, κ

Application

ELISA

Basic Information

Immunogen

DUSP16 (NP_085143.1, 561 a.a. ~ 665 a.a) partial recombinant protein with GST tag. The immunogen sequence: TESSHFYSAS AIYGGSASYS AYSCSQLPTC GDQVYSVRRR QKPSDRADSR RSWHEESPFE KQFKRRSCQM EFGESIMSEN RSREELGKVG SQSSFSGSME IIEVS

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 561-665

Target

Full Name

Dual Specificity Phosphatase 16

Introduction

DUSP16 is a mitogen-activated protein kinase phosphatase that is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. The encoded protein specifically regulates the c-Jun amino-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) pathways.

Entrez Gene ID

80824

UniProt ID

Q9BY84

Alternative Names

Dual Specificity Phosphatase 16; Mitogen-Activated Protein Kinase Phosphatase 7; MAP Kinase Phosphatase 7; MAPK Phosphatase-7; MKP-7; MKP7;

Research Area

Dual specificity protein phosphatase involved in the inactivation of MAP kinases. Dephosphorylates MAPK10 bound to ARRB2.

Biological Process

Dephosphorylation Source: UniProtKB
Inactivation of MAPK activity Source: GO_Central
MAPK export from nucleus Source: UniProtKB
MAPK phosphatase export from nucleus, leptomycin B sensitive Source: UniProtKB
Peptidyl-threonine dephosphorylation Source: GO_Central
Peptidyl-tyrosine dephosphorylation Source: GO_Central
Protein dephosphorylation Source: GO_Central

Cellular Location

Nucleus; Cytoplasm; Cytoplasmic vesicle. After dissociation upon AGTR stimulation, re-associates with ARRB2 on endocytic vesicles.

PTM

Phosphorylated at Ser-446 by MAPK1/ERK2, which prevents its degradation, and thereby stabilizes it and blocks JNK MAPK activity.
(Microbial infection) Acetylated at Lys-55 by the M.tuberculosis Eis protein; this leads to the inhibition of JNK-dependent autophagy, phagosome maturation, and ROS (reactive oxygen species) generation for enhanced intracellular survival of M.tuberculosis.

More Infomation

References

Low, H. B., Wong, Z. L., Wu, B., Kong, L. R., Png, C. W., Cho, Y. L., ... & Zhang, Y. (2021). DUSP16 promotes cancer chemoresistance through regulation of mitochondria-mediated cell death. Nature communications, 12(1), 1-16.

Liu, W., Wang, B., Duan, A., Shen, K., Zhang, Q., Tang, X., ... & Zhang, S. (2021). Exosomal transfer of miR-769-5p promotes osteosarcoma proliferation and metastasis by targeting DUSP16. Cancer cell international, 21(1), 1-17.

Wu, Y. K., Hu, L. F., Lou, D. S., Wang, B. C., & Tan, J. (2020). Targeting DUSP16/TAK1 signaling alleviates hepatic dyslipidemia and inflammation in high fat diet (HFD)-challenged mice through suppressing JNK MAPK. Biochemical and Biophysical Research Communications, 524(1), 142-149.

Li, J. M., Zhou, J., Xu, Z., Huang, H. J., Chen, M. J., & Ji, J. S. (2018). MicroRNA‐27a‐3p inhibits cell viability and migration through down‐regulating DUSP16 in hepatocellular carcinoma. Journal of cellular biochemistry, 119(7), 5143-5152.

Zega, K., Jovanovic, V. M., Vitic, Z., Niedzielska, M., Knaapi, L., Jukic, M. M., ... & Brodski, C. (2017). Dusp16 deficiency causes congenital obstructive hydrocephalus and brain overgrowth by expansion of the neural progenitor pool. Frontiers in Molecular Neuroscience, 10, 372.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Alternative Versions

Mouse Anti-DUSP16 Recombinant Antibody (3F3)

Related Products

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Rabbit Anti-DUSP16 Recombinant Antibody (D1885)

Mouse Anti-DUSP16 Recombinant Antibody (3F3)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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