Rabbit Anti-DUSP16 Recombinant Antibody (D1885) (V2LY-0425-LY1061)

Name: Rabbit Anti-DUSP16 Recombinant Antibody (D1885)
SKU: V2LY-0425-LY1061
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Rabbit

Specificity

Human, Mouse, Rat, Monkey

Clone

D1885

Antibody Isotype

IgG

Application

Basic Information

Immunogen

Synthetic peptide corresponding to residues surrounding Lys431 of human DUSP16 protein.

Host Species

Rabbit

Specificity

Human, Mouse, Rat, Monkey

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

BSA & Glycerol & HEPES

Preservative

Sodium Azide

Concentration

Batch dependent

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Dual Specificity Phosphatase 16

Entrez Gene ID

Human	80824
Mouse	70686
Rat	297682
Monkey	696332

UniProt ID

Human	Q9BY84
Mouse	Q920R2
Rat	D4A3W6
Monkey	F6YFQ2

Research Area

Dual specificity protein phosphatase involved in the inactivation of MAP kinases. Dephosphorylates MAPK10 bound to ARRB2.

Biological Process

Dephosphorylation Source: UniProtKB
Inactivation of MAPK activity Source: GO_Central
MAPK export from nucleus Source: UniProtKB
MAPK phosphatase export from nucleus, leptomycin B sensitive Source: UniProtKB
Peptidyl-threonine dephosphorylation Source: GO_Central
Peptidyl-tyrosine dephosphorylation Source: GO_Central
Protein dephosphorylation Source: GO_Central

Cellular Location

Nucleus; Cytoplasm; Cytoplasmic vesicle. After dissociation upon AGTR stimulation, re-associates with ARRB2 on endocytic vesicles.

PTM

Phosphorylated at Ser-446 by MAPK1/ERK2, which prevents its degradation, and thereby stabilizes it and blocks JNK MAPK activity.
(Microbial infection) Acetylated at Lys-55 by the M.tuberculosis Eis protein; this leads to the inhibition of JNK-dependent autophagy, phagosome maturation, and ROS (reactive oxygen species) generation for enhanced intracellular survival of M.tuberculosis.

More Infomation

References

Low, H. B., Wong, Z. L., Wu, B., Kong, L. R., Png, C. W., Cho, Y. L., ... & Zhang, Y. (2021). DUSP16 promotes cancer chemoresistance through regulation of mitochondria-mediated cell death. Nature communications, 12(1), 1-16.

Liu, W., Wang, B., Duan, A., Shen, K., Zhang, Q., Tang, X., ... & Zhang, S. (2021). Exosomal transfer of miR-769-5p promotes osteosarcoma proliferation and metastasis by targeting DUSP16. Cancer cell international, 21(1), 1-17.

Wu, Y. K., Hu, L. F., Lou, D. S., Wang, B. C., & Tan, J. (2020). Targeting DUSP16/TAK1 signaling alleviates hepatic dyslipidemia and inflammation in high fat diet (HFD)-challenged mice through suppressing JNK MAPK. Biochemical and Biophysical Research Communications, 524(1), 142-149.

Li, J. M., Zhou, J., Xu, Z., Huang, H. J., Chen, M. J., & Ji, J. S. (2018). MicroRNA‐27a‐3p inhibits cell viability and migration through down‐regulating DUSP16 in hepatocellular carcinoma. Journal of cellular biochemistry, 119(7), 5143-5152.

Zega, K., Jovanovic, V. M., Vitic, Z., Niedzielska, M., Knaapi, L., Jukic, M. M., ... & Brodski, C. (2017). Dusp16 deficiency causes congenital obstructive hydrocephalus and brain overgrowth by expansion of the neural progenitor pool. Frontiers in Molecular Neuroscience, 10, 372.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Rabbit Anti-DUSP16 Recombinant Antibody (D5F4)

Mouse Anti-DUSP16 Recombinant Antibody (3F3)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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