Mouse Anti-ECSIT Recombinant Antibody (CBFYE-0416) (CBMAB-E0731-FY)

Name: Mouse Anti-ECSIT Recombinant Antibody (CBFYE-0416)
SKU: CBMAB-E0731-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-0416

Application

ELISA, PLA

Immunogen

ECSIT (aa20-431) full-length recombinant protein.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 20-431

More Infomation

Target

Full Name

ECSIT homolog (Drosophila)

Introduction

ECSIT (ECSIT Signalling Integrator) is a Protein Coding gene. Diseases associated with ECSIT include Dental Pulp Disease. Among its related pathways are Respiratory electron transport, ATP synthesis by chemiosmotic coupling, and heat production by uncoupling proteins. and Innate Immune System. Gene Ontology (GO) annotations related to this gene include DNA binding transcription factor activity and oxidoreductase activity, acting on NAD(P)H.

Entrez Gene ID

51295

UniProt ID

Q9BQ95

Alternative Names

ECSIT Signalling Integrator; Likely Ortholog Of Mouse Signaling Intermediate In Toll Pathway Evolutionarily Conserved; Signaling Intermediate In Toll Pathway Evolutionarily Conserved Ortholog (Mouse); Evolutionarily Conserved Signaling Intermediate In Toll Pathway, Mitochondrial; ECSIT Homolog (Drosophila); Protein SITPEC; ECSIT Homolog; SITPEC

Research Area

Adapter protein of the Toll-like and IL-1 receptor signaling pathway that is involved in the activation of NF-kappa-B via MAP3K1. Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development (By similarity).

Required for efficient assembly of mitochondrial NADH:ubiquinone oxidoreductase.

Biological Process

Innate immune response Source: UniProtKB-KW
Regulation of oxidoreductase activity Source: UniProtKB
Regulation of protein complex stability Source: UniProtKB

Cellular Location

Nucleus; Mitochondrion; Cytoplasm

Chaitanya, N. S., Tammineni, P., Nagaraju, G. P., & Reddy, A. B. (2022). Pleiotropic roles of evolutionarily conserved signaling intermediate in toll pathway (ECSIT) in pathophysiology. Journal of Cellular Physiology.

Qi, Z., Pi, X., Xu, Y., Zhang, Q., Wangkahart, E., Meng, F., & Wang, Z. (2022). Molecular characterization of the evolutionary conserved signaling intermediate in Toll pathways (ECSIT) of soiny mullet (Liza haematocheila). Fish & Shellfish Immunology.

Hu, Y., Liu, W., Zhang, X., & Liu, D. (2022). ECSIT inhibits cell death to increase tumor progression and metastasis via p53 in human breast cancer. Translational Cancer Research, 11(4), 699.

Xu, L., Humphries, F., Delagic, N., Wang, B., Holland, A., Edgar, K. S., ... & Moynagh, P. N. (2021). ECSIT is a critical limiting factor for cardiac function. JCI insight, 6(12).

Ge, Q., Wang, J., Li, J., & Li, J. (2021). Identification, characterization, and functional analysis of Toll and ECSIT in Exopalaemon carinicauda. Developmental & Comparative Immunology, 116, 103926.

Kim, M. J., Min, Y., Kwon, J., Son, J., Im, J. S., Shin, J., & Lee, K. Y. (2019). p62 negatively regulates TLR4 signaling via functional regulation of the TRAF6-ECSIT complex. Immune Network, 19(3).

Kim, M. J., Min, Y., Shim, J. H., Chun, E., & Lee, K. Y. (2019). CRBN is a negative regulator of bactericidal activity and autophagy activation through inhibiting the ubiquitination of ECSIT and BECN1. Frontiers in immunology, 10, 2203.

Wen, H., Ma, H., Cai, Q., Lin, S., Lei, X., He, B., ... & Liu, Q. (2018). Recurrent ECSIT mutation encoding V140A triggers hyperinflammation and promotes hemophagocytic syndrome in extranodal NK/T cell lymphoma. Nature medicine, 24(2), 154-164.

Carneiro, F. R., Lepelley, A., Seeley, J. J., Hayden, M. S., & Ghosh, S. (2018). An essential role for ECSIT in mitochondrial complex I assembly and mitophagy in macrophages. Cell reports, 22(10), 2654-2666.

Wang, H., Behrens, E. M., Pignolo, R. J., & Kaplan, F. S. (2018). ECSIT links TLR and BMP signaling in FOP connective tissue progenitor cells. Bone, 109, 201-209.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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