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Rabbit Anti-EHD1 Recombinant Antibody (E0131) (V2LY-0425-LY1594)


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Summary

Host Animal
Rabbit
Specificity
Mouse, Rat, Human
Clone
E0131
Antibody Isotype
IgG
Application
WB, IP, IHC-P, IF, FC

Basic Information

Immunogen
A synthesized peptide derived from human EHD1.
Host Species
Rabbit
Specificity
Mouse, Rat, Human
Antibody Isotype
IgG
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
IP1:50
IF(ICC)1:50-1:200
IHC-P1:50-1:200
FC1:40

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
BSA & Glycerol & PBS
Preservative
Sodium Azide
Concentration
Batch dependent
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
EH Domain Containing 1
Entrez Gene ID
Human10938
Mouse13660
Rat293692
UniProt ID
HumanQ9H4M9
MouseQ9WVK4
RatQ641Z6
Research Area
ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes vesiculation of endocytic membranes (PubMed:24019528).

Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes (PubMed:15020713, PubMed:17233914, PubMed:20801876).

Recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth (By similarity).

Plays a role in myoblast fusion (By similarity).

Involved in the unidirectional retrograde dendritic transport of endocytosed BACE1 and in efficient sorting of BACE1 to axons implicating a function in neuronal APP processing (By similarity).

Plays a role in the formation of the ciliary vesicle (CV), an early step in cilium biogenesis. Proposed to be required for the fusion of distal appendage vesicles (DAVs) to form the CV by recruiting SNARE complex component SNAP29. Is required for recruitment of transition zone proteins CEP290, RPGRIP1L, TMEM67 and B9D2, and of IFT20 following DAV reorganization before Rab8-dependent ciliary membrane extension. Required for the loss of CCP110 form the mother centriole essential for the maturation of the basal body during ciliogenesis (PubMed:25686250).
Biological Process
Cellular response to nerve growth factor stimulus Source: UniProtKB
Cholesterol homeostasis Source: BHF-UCL
Cilium assembly Source: UniProtKB
Endocytic recycling Source: UniProtKB
Endocytosis Source: UniProtKB
Endosomal transport Source: GO_Central
Intracellular protein transport Source: UniProtKB
Low-density lipoprotein particle clearance Source: BHF-UCL
Neuron projection development Source: UniProtKB
Positive regulation of cholesterol storage Source: BHF-UCL
Positive regulation of endocytic recycling Source: UniProtKB
Positive regulation of myoblast fusion Source: UniProtKB
Positive regulation of neuron projection development Source: Ensembl
Protein homooligomerization Source: UniProtKB
Protein localization to cilium Source: UniProtKB
Protein localization to plasma membrane Source: GO_Central
Cellular Location
Cell membrane; Cilium membrane; Recycling endosome membrane; Early endosome membrane. Preferentially associates with tubular recycling endosomes (PubMed:15020713, PubMed:17233914, PubMed:19864458, PubMed:23596323). Colocalizes with FER1L5 at plasma membrane in myoblasts and myotubes (By similarity). Localizes to the ciliary pocket from where the cilium protrudes (PubMed:25686250). Colocalizes with BACE1 in tubulovesicular cytoplasmic membranes. Colocalizes with BACE1 and APP amyloid beta proteins in hippocampal mossy fiber terminals (By similarity).
More Infomation

Santos, H. J., Hanadate, Y., Imai, K., Watanabe, H., & Nozaki, T. (2022). Entamoeba histolytica EHD1 is involved in mitosome-endosome contact. Mbio, 13(2), e03849-21.

Lu, Y., Wang, W., & Tan, S. (2022). EHD1 promotes the cancer stem cell (CSC)‐like traits of glioma cells via interacting with CD44 and suppressing CD44 degradation. Environmental Toxicology, 37(9), 2259-2268.

Liu, Y., Song, Y., Cao, M., Fan, W., Cui, Y., Cui, Y., ... & Xing, Y. (2022). A novel EHD1/CD44/Hippo/SP1 positive feedback loop potentiates stemness and metastasis in lung adenocarcinoma. Clinical and Translational Medicine, 12(4), e836.

Lin, Y. S., Huang, K. Y., Yu, H. C., Lu, M. C., Fan, C. J., Tseng, H. Y. H., ... & Huang, H. B. (2020). Identification of phostensin in association with Eps 15 homology domain-containing protein 1 (EHD1) and EHD4. Biochemical and Biophysical Research Communications, 531(2), 236-241.

Jones, T., Naslavsky, N., & Caplan, S. (2020). Eps15 Homology Domain Protein 4 (EHD4) is required for Eps15 Homology Domain Protein 1 (EHD1)-mediated endosomal recruitment and fission. PloS one, 15(9), e0239657.

Dhawan, K., Naslavsky, N., & Caplan, S. (2020). Sorting nexin 17 (SNX17) links endosomal sorting to Eps15 homology domain protein 1 (EHD1)–mediated fission machinery. Journal of Biological Chemistry, 295(12), 3837-3850.

Wu, C., Bao, G., Xu, G., Sun, Y., Wang, L., Chen, J., ... & Cui, Z. (2019). Triad1 regulates the expression and distribution of EHD1 contributing to the neurite outgrowth of neurons after spinal cord injury. Journal of Cellular Biochemistry, 120(4), 5355-5366.

Liu, Y., Liang, Y., Li, M., Liu, D., Tang, J., Yang, W., ... & Jin, X. (2018). Eps15 homology domain 1 promotes the evolution of papillary thyroid cancer by regulating endocytotic recycling of epidermal growth factor receptor. Oncology Letters, 16(4), 4263-4270.

Kamens, A. J., Mientkiewicz, K. M., Eisert, R. J., Walz, J. A., Mace, C. R., & Kritzer, J. A. (2018). Thioether-stapled macrocyclic inhibitors of the EH domain of EHD1. Bioorganic & medicinal chemistry, 26(6), 1206-1211.

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For research use only. Not intended for any clinical use.

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