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Mouse Anti-EIF5 Recombinant Antibody (1D9-4B9) (CBMAB-A2551-LY)

The product is antibody recognizes EIF5. The antibody 1D9-4B9 immunoassay techniques such as: WB, ELISA.
See all EIF5 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1D9-4B9
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
EIF5 (AAH32866, 1 a.a. ~ 431 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Eukaryotic Translation Initiation Factor 5
Entrez Gene ID
1983
UniProt ID
P55010
Alternative Names
EIF-5; EIF-5A
Research Area
Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).
Biological Process
Activation of GTPase activity Source: Ensembl
Formation of cytoplasmic translation initiation complex Source: GO_Central
Formation of translation preinitiation complex Source: GO_Central
Regulation of translational initiation Source: CACAO
Cellular Location
Cytosol; Plasma membrane; Cytoplasm

Li, Z., Wu, Y., Fu, Y., Chen, X., Zhao, X., Wu, X., ... & Sun, F. (2022). Cyst stem cell lineage eIF5 non-autonomously prevents testicular germ cell tumor formation via eIF1A/eIF2γ-mediated pre-initiation complex. Stem cell research & therapy, 13(1), 1-18.

Paul, E. E., Lin, K. Y., Gamble, N., Tsai, A. W. L., Swan, S. H., Yang, Y., ... & Marintchev, A. (2022). Dynamic interaction network involving the conserved intrinsically disordered regions in human eIF5. Biophysical Chemistry, 281, 106740.

Singh, C. R., Jaiswal, R., Escalante, C. R., & Asano, K. (2022). Label-free protocol to quantify protein affinity using isothermal titration calorimetry and bio-layer interferometry of a human eIF5-mimic protein. STAR protocols, 3(3), 101615.

Marintchev, A., Paul, E. E., Lin, K. Y., Tsai, A. W. L., Yang, Y., Gamble, N., & Doran, M. (2022). Dynamic Interaction Network Involving the Conserved Intrinsically Disordered Regions in Human eIF5. The FASEB Journal, 36.

Cai, S. X., Chen, W. S., Zeng, W., Cheng, X. F., Lin, M. B., & Wang, J. S. (2021). Roles of HDAC2, eIF5, and eIF6 in lung cancer tumorigenesis. Current medical science, 41(4), 764-769.

Zeman, J., Itoh, Y., Kukačka, Z., Rosůlek, M., Kavan, D., Kouba, T., ... & Valášek, L. S. (2019). Binding of eIF3 in complex with eIF5 and eIF1 to the 40S ribosomal subunit is accompanied by dramatic structural changes. Nucleic acids research, 47(15), 8282-8300.

Lin, K. Y., Nag, N., Pestova, T. V., & Marintchev, A. (2018). Human eIF5 and eIF1A compete for binding to eIF5B. Biochemistry, 57(40), 5910-5920.

Llácer, J. L., Hussain, T., Saini, A. K., Nanda, J. S., Kaur, S., Gordiyenko, Y., ... & Ramakrishnan, V. (2018). Translational initiation factor eIF5 replaces eIF1 on the 40S ribosomal subunit to promote start-codon recognition. Elife, 7, e39273.

Ross, J. A., Bressler, K. R., & Thakor, N. (2018). Eukaryotic initiation factor 5B (eIF5B) cooperates with eIF1A and eIF5 to facilitate uORF2-mediated repression of ATF4 translation. International journal of molecular sciences, 19(12), 4032.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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