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Mouse Anti-ELK1 Recombinant Antibody (2G6) (CBMAB-A2574-LY)

The product is antibody recognizes ELK1. The antibody 2G6 immunoassay techniques such as: sELISA, ELISA.
See all ELK1 antibodies
Published Data
Fig. 1 Western blot analysis of HIF-1α, HIF-2α, and ELK protein expression in Hep3B cells or cells transfected with control, HIF-1α, HIF-2α, or ELK siRNAs at 21 or 1.5% O₂ (H).
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Summary

Host Animal
Mouse
Specificity
Human
Clone
2G6
Antibody Isotype
IgG2a, κ
Application
sELISA, ELISA

Basic Information

Immunogen
ELK1 (NP_005220, 67 a.a. ~ 166 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
ELK1, ETS Transcription Factor
Introduction
This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
Entrez Gene ID
2002
UniProt ID
P19419
Research Area
Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. Induces target gene transcription upon JNK-signaling pathway stimulation (By similarity).
Biological Process
Cell differentiation Source: GO_Central
Cellular response to gamma radiation Source: Ensembl
Cellular response to testosterone stimulus Source: Ensembl
Gene expression Source: Ensembl
Liver development Source: Ensembl
Lung development Source: Ensembl
Positive regulation of neuron death Source: Ensembl
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Response to fibroblast growth factor Source: UniProtKB
Response to light stimulus Source: Ensembl
Cellular Location
Nucleus
PTM
Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Also phosphorylated on Ser-383 by MAPK8 and/or MAKP9. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. Phosphorylated and activated by CAMK4, MAPK11, MAPK12 and MAPK14. Upon bFGF stimulus, phosphorylated by PAK1 (By similarity).

Wang, B., Xu, Z., Wang, X., Xia, S., Cai, P., Wang, M., & Gao, Z. (2022). Knockdown of lncRNA LINC00662 suppresses malignant behaviour of osteosarcoma cells via competition with miR-30b-3p to regulate ELK1 expression. Journal of orthopaedic surgery and research, 17(1), 1-14.

Li, X., Lu, L., Hou, W., Wang, F., Huang, T., Meng, Z., & Zhu, M. (2022). The SETD8/ELK1/bach1 complex regulates hyperglycaemia-mediated EndMT in diabetic nephropathy. Journal of translational medicine, 20(1), 1-18.

Li, Z., Cheng, Y., Fu, K., Lin, Q., Zhao, T., Tang, W., ... & Sun, Y. (2021). Circ-PTPDC1 promotes the progression of gastric cancer through sponging Mir-139-3p by regulating ELK1 and functions as a prognostic biomarker. International journal of biological sciences, 17(15), 4285.

Li, P., Ge, D., Li, P., Hu, F., Chu, J., Chen, X., ... & Gu, X. (2020). CXXC finger protein 4 inhibits the CDK18‐ERK1/2 axis to suppress the immune escape of gastric cancer cells with involvement of ELK1/MIR100HG pathway. Journal of cellular and molecular medicine, 24(17), 10151-10165.

Zhao, G., & Dai, G. J. (2020). Hsa_circRNA_000166 promotes cell proliferation, migration and invasion by regulating miR-330-5p/ELK1 in colon cancer. OncoTargets and therapy, 13, 5529.

Yang, R., Li, X., Wu, Y., Zhang, G., Liu, X., Li, Y., ... & Cui, H. (2020). EGFR activates GDH1 transcription to promote glutamine metabolism through MEK/ERK/ELK1 pathway in glioblastoma. Oncogene, 39(14), 2975-2986.

Gao, Y., Zhou, J., Xie, Z., Wang, J., Ho, C. K., Zhang, Y., & Li, Q. (2019). Mechanical strain promotes skin fibrosis through LRG-1 induction mediated by ELK1 and ERK signalling. Communications biology, 2(1), 1-15.

Tang, Q., Chen, Z., & Zhao, L. (2019). Circular RNA hsa_circ_0000515 acts as a miR-326 sponge to promote cervical cancer progression through up-regulation of ELK1. Aging (Albany NY), 11(22), 9982.

Huang, B., Chang, C., Wang, B. L., & Li, H. (2019). ELK1‐induced upregulation of lncRNA TRPM2‐AS promotes tumor progression in gastric cancer by regulating miR‐195/HMGA1 axis. Journal of Cellular Biochemistry, 120(10), 16921-16933.

Wang, Y., Wang, K., Hu, Z., Zhou, H., Zhang, L., Wang, H., ... & Shi, F. (2018). MicroRNA-139-3p regulates osteoblast differentiation and apoptosis by targeting ELK1 and interacting with long noncoding RNA ODSM. Cell death & disease, 9(11), 1-16.

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For research use only. Not intended for any clinical use.

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