Mouse Anti-ELMO1 Recombinant Antibody (CP0579) (V2LY-0825-LY98)

Name: Mouse Anti-ELMO1 Recombinant Antibody (CP0579)
SKU: V2LY-0825-LY98
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CP0579

Application

WB, IP, IF, ELISA

Immunogen

Full length of mouse Elmo1.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Engulfment And Cell Motility 1

Entrez Gene ID

Human	9844
Mouse	140580
Rat	361251

UniProt ID

Human	Q92556
Mouse	Q8BPU7
Rat	D3ZY46

Research Area

Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.

Biological Process

Actin cytoskeleton organization Source: UniProtKB
Actin filament organization Source: GO_Central
Apoptotic process Source: UniProtKB
Cell migration Source: InterPro
Cell motility Source: UniProtKB
Phagocytosis, engulfment Source: UniProtKB
Rac protein signal transduction Source: UniProtKB
Regulation of catalytic activity Source: GOC

Cellular Location

Cell membrane; Cytoplasm. Translocation to plasma membrane seems to be mediated by DOCK1 and CRK.

PTM

Phosphorylated by HCK.

Tocci, S., Ibeawuchi, S. R., Das, S., & Sayed, I. M. (2022). Role of ELMO1 in inflammation and cancer—clinical implications. Cellular Oncology, 1-21.

Kohorn, B. D., Zorensky, F. D., Dexter-Meldrum, J., Chabout, S., Mouille, G., & Kohorn, S. (2021). Mutation of an Arabidopsis Golgi membrane protein ELMO1 reduces cell adhesion. Development, 148(10), dev199420.

Arandjelovic, S., Perry, J. S., Zhou, M., Ceroi, A., Smirnov, I., Walk, S. F., ... & Ravichandran, K. S. (2021). ELMO1 signaling is a promoter of osteoclast function and bone loss. Nature communications, 12(1), 1-12.

Kukimoto-Niino, M., Katsura, K., Kaushik, R., Ehara, H., Yokoyama, T., Uchikubo-Kamo, T., ... & Shirouzu, M. (2021). Cryo-EM structure of the human ELMO1-DOCK5-Rac1 complex. Science Advances, 7(30), eabg3147.

Xie, Z., Yu, W., Zheng, G., Li, J., Cen, S., Ye, G., ... & Shen, H. (2021). TNF-α-mediated m6A modification of ELMO1 triggers directional migration of mesenchymal stem cell in ankylosing spondylitis. Nature communications, 12(1), 1-14.

Chang, L., Yang, J., Jo, C. H., Boland, A., Zhang, Z., McLaughlin, S. H., ... & Barford, D. (2020). Structure of the DOCK2− ELMO1 complex provides insights into regulation of the auto-inhibited state. Nature communications, 11(1), 1-17.

Arandjelovic, S., Perry, J. S., Lucas, C. D., Penberthy, K. K., Kim, T. H., Zhou, M., ... & Ravichandran, K. S. (2019). A noncanonical role for the engulfment gene ELMO1 in neutrophils that promotes inflammatory arthritis. Nature immunology, 20(2), 141-151.

Peng, H., Zhang, Y., Zhou, Z., Guo, Y., Huang, X., Westover, K. D., ... & Shen, S. (2019). Intergrated analysis of ELMO1, serves as a link between tumour mutation burden and epithelial-mesenchymal transition in hepatocellular carcinoma. EBioMedicine, 46, 105-118.

Luo, T., Chen, M., Zhao, Y., Wang, D., Liu, J., Chen, J., ... & Li, L. (2019). Macrophage-associated lncRNA ELMO1-AS1: a novel therapeutic target and prognostic biomarker for hepatocellular carcinoma. OncoTargets and therapy, 12, 6203.

Li, W., Tam, K. M. V., Chan, W. W. R., Koon, A. C., Ngo, J. C. K., Chan, H. Y. E., & Lau, K. F. (2018). Neuronal adaptor FE65 stimulates Rac1-mediated neurite outgrowth by recruiting and activating ELMO1. Journal of Biological Chemistry, 293(20), 7674-7688.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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