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Mouse Anti-EPAS1 Recombinant Antibody (1G2) (CBMAB-A2631-LY)

The product is antibody recognizes EPAS1. The antibody 1G2 immunoassay techniques such as: WB, ELISA.
See all EPAS1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1G2
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
EPAS1 (NP_001421.2, 28 a.a. ~ 347 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
EPAS1
Entrez Gene ID
2034
UniProt ID
Q99814
Alternative Names
ECYT4; HIF2A; HLF; MOP2; PASD2; bHLHe73
Research Area
Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity).

Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX1 seems to activate CTAD (By similarity).
Biological Process
Angiogenesis Source: UniProtKB-KW
Blood vessel remodeling Source: Ensembl
Cell maturation Source: Ensembl
Cellular response to hypoxia Source: UniProtKB
Embryonic placenta development Source: Ensembl
Erythrocyte differentiation Source: Ensembl
Iron ion homeostasis Source: Ensembl
Lung development Source: Ensembl
Mitochondrion organization Source: Ensembl
Myoblast fate commitment Source: BHF-UCL
Norepinephrine metabolic process Source: Ensembl
Positive regulation of cold-induced thermogenesis Source: YuBioLab
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Regulation of heart rate Source: Ensembl
Regulation of protein neddylation Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Regulation of transcription from RNA polymerase II promoter in response to oxidative stress Source: Ensembl
Response to hypoxia Source: MGI
Signal transduction Source: ProtInc
Surfactant homeostasis Source: Ensembl
Visual perception Source: Ensembl
Cellular Location
Nucleus; Nucleus speckle. Colocalizes with HIF3A in the nucleus and speckles.
Involvement in disease
Erythrocytosis, familial, 4 (ECYT4):
An autosomal dominant disorder characterized by elevated serum hemoglobin and hematocrit, and normal platelet and leukocyte counts.
PTM
In normoxia, is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization (By similarity).By similarity
In normoxia, is hydroxylated on Asn-847 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation.By similarity
Phosphorylated on multiple sites in the CTAD.By similarity
The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains.By similarity

Gao, Z., Xiao, J., Li, X., & Li, C. (2022). Hypoxia promotes erythroid differentiation of human K562 cells by upregulating endothelial PAS domain-containing protein 1 (EPAS1) expression. Xi bao yu fen zi Mian yi xue za zhi= Chinese Journal of Cellular and Molecular Immunology, 38(6), 494-500.

Zhang, M., Li, J., Lin, W., Qi, L., Yao, C., Zheng, Z., ... & Qi, Y. (2022). EPAS1 Promoter Hypermethylation is a Diagnostic and Prognostic Biomarker for Non-Small Cell Lung Cancer. Genetic Testing and Molecular Biomarkers, 26(7-8), 360-374.

Dong, J., Li, S., Lu, Z., Du, P., Liu, G., Li, M., ... & Bao, J. (2022). HCMV-miR-US33-5p promotes apoptosis of aortic vascular smooth muscle cells by targeting EPAS1/SLC3A2 pathway. Cellular & Molecular Biology Letters, 27(1), 1-17.

Wang, S., Wang, Y., Liu, C., Xu, G., Gao, W., Hao, J., ... & Gao, Y. (2021). EPAS1 (Endothelial PAS Domain Protein 1) Orchestrates Transactivation of Endothelial ICAM1 (Intercellular Adhesion Molecule 1) by Small Nucleolar RNA Host Gene 5 (SNHG5) to Promote Hypoxic Pulmonary Hypertension. Hypertension, 78(4), 1080-1091.

Hu, A., Yang, L. Y., Liang, J., Lu, D., Zhang, J. L., Cao, F. F., ... & Zhang, J. F. (2020). SIRT2 modulates VEGFD‐associated lymphangiogenesis by deacetylating EPAS1 in human head and neck cancer. Molecular Carcinogenesis, 59(11), 1280-1291.

Schelker, R. C., Herr, W., & Grassinger, J. (2019). A new exon 12 mutation in the EPAS1 gene possibly associated with erythrocytosis. European Journal of Haematology, 103(1), 64-66.

Sergi, C. (2019). EPAS 1, congenital heart disease, and high altitude: disclosures by genetics, bioinformatics, and experimental embryology. Bioscience Reports, 39(5).

Pan, R., Zhou, C., Dai, J., Ying, X., Yu, H., Zhong, J., ... & Duan, S. (2018). Endothelial PAS domain protein 1 gene hypomethylation is associated with colorectal cancer in Han Chinese. Experimental and Therapeutic Medicine, 16(6), 4983-4990.

Xu, X. H., Bao, Y., Wang, X., Yan, F., Guo, S., Ma, Y., ... & Wang, J. (2018). Hypoxic‐stabilized EPAS1 proteins transactivate DNMT1 and cause promoter hypermethylation and transcription inhibition of EPAS1 in non‐small cell lung cancer. The FASEB Journal, 32(12), 6694-6705.

Crawford, N. F., Coleman, S. J., Holt, T. N., Speidel, S. E., Enns, R. M., Hamid, R., & Thomas, M. G. (2018). Allele distribution and testing for association between an oxygen dependent degradation domain SNP in EPAS1 and pulmonary arterial pressures in yearling Angus cattle. Agri Gene, 9, 27-31.

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For research use only. Not intended for any clinical use.

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