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Mouse Anti-EPHA8 Recombinant Antibody (CBT487) (V2LY-0625-LY3485)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBT487
Antibody Isotype
IgG
Application
WB, ELISA

Basic Information

Immunogen
Purified recombinant fragment of EphA8 (aa70-150) expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
ELISA1:10,000
WB1:500-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Buffer
BSA, Glycerol
Preservative
Sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
EPH Receptor A8
Entrez Gene ID
2046
UniProt ID
P29322
Research Area
Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. The GPI-anchored ephrin-A EFNA2, EFNA3, and EFNA5 are able to activate EPHA8 through phosphorylation. With EFNA5 may regulate integrin-mediated cell adhesion and migration on fibronectin substrate but also neurite outgrowth. During development of the nervous system plays also a role in axon guidance. Downstream effectors of the EPHA8 signaling pathway include FYN which promotes cell adhesion upon activation by EPHA8 and the MAP kinases in the stimulation of neurite outgrowth (By similarity).
Biological Process
Axon guidance Source: UniProtKB
Cell adhesion Source: UniProtKB-KW
Cellular response to follicle-stimulating hormone stimulus Source: Ensembl
Ephrin receptor signaling pathway Source: UniProtKB
Neuron projection development Source: UniProtKB
Neuron remodeling Source: UniProtKB
Positive regulation of kinase activity Source: GO_Central
Positive regulation of MAPK cascade Source: UniProtKB
Positive regulation of phosphatidylinositol 3-kinase activity Source: UniProtKB
Protein autophosphorylation Source: UniProtKB
Regulation of cell adhesion Source: UniProtKB
Regulation of cell adhesion mediated by integrin Source: UniProtKB
Substrate-dependent cell migration Source: UniProtKB
Transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Cellular Location
Cell membrane; Early endosome membrane; Cell projection. Undergoes clathrin-mediated endocytosis upon EFNA5-binding and is targeted to early endosomes.
Topology
Extracellular: 28-542
Helical: 543-563
Cytoplasmic: 564-1005
PTM
Phosphorylated. Phosphorylation is stimulated upon binding of its ligands including EFNA2, EFNA3 and EFNA5. Autophosphorylation on Tyr-616 is critical for association with FYN. Autophosphorylation on Tyr-839 modulates tyrosine kinase activity (By similarity).
Ubiquitinated. Ubiquitination by CBL regulates the receptor stability and activity through proteasomal degradation. ANKS1A prevents ubiquitination and degradation (By similarity).
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For research use only. Not intended for any clinical use.

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