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Mouse Anti-EPHX2 Recombinant Antibody (CBFYE-1040) (CBMAB-E1553-FY)

This product is mouse antibody that recognizes EPHX2. The antibody CBFYE-1040 can be used for immunoassay techniques such as: FC, IF, WB.
See all EPHX2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYE-1040
Antibody Isotype
IgG1
Application
FC, IF, WB

Basic Information

Immunogen
EPHX2 antibody was raised in mouse using a full length recombinant protein of human EPHX2 produced in HEK293T cells, as the immunogen
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.3, 1%BSA, 50% glycerol
Preservative
0.02% Sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Epoxide Hydrolase 2
Introduction
This gene encodes a member of the epoxide hydrolase family. The protein, found in both the cytosol and peroxisomes, binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have been associated with familial hypercholesterolemia. Alternatively spliced transcript variants have been described.
Entrez Gene ID
2053
UniProt ID
P34913
Alternative Names
Epoxide Hydrolase 2; Epoxide Hydrolase 2, Cytoplasmic; Bifunctional Epoxide Hydrolase 2; Epoxide Hydrolase 2, Cytosolic; Epoxide Hydrolase, Soluble; Epoxide Hydratase; CEH; SEH
Research Area
Bifunctional enzyme (PubMed:12574510).

The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides (PubMed:12869654, PubMed:12574510, PubMed:22798687).

Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides (By similarity).

Also determines steady-state levels of physiological mediators (PubMed:12869654, PubMed:12574510, PubMed:22798687, PubMed:21217101).

Bifunctional enzyme (PubMed:12574510).

The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo-9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro-9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy-octadec-9Z-enoic acid and 12-phosphonooxy-octadec-9E-enoic acid (PubMed:12574510).

Has phosphatase activity toward lyso-glycerophospholipids with also some lower activity toward lysolipids of sphingolipid and isoprenoid phosphates (PubMed:22217705, PubMed:22387545).
Biological Process
Cholesterol homeostasis Source: UniProtKB
Dephosphorylation Source: UniProtKB
Epoxide metabolic process Source: UniProtKB
Phospholipid dephosphorylation Source: UniProtKB
Positive regulation of gene expression Source: UniProtKB
Regulation of cholesterol metabolic process Source: UniProtKB
Response to toxic substance Source: UniProtKB-KW
Stilbene catabolic process Source: UniProtKB
Cellular Location
Cytoplasm; Peroxisome
PTM
The N-terminus is blocked.
The covalent modification of cysteine by 15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic and reversible. It may occur as an alternative to other cysteine modifications, such as S-nitrosylation and S-palmitoylation (Probable).

Roumeliotis, S., Roumeliotis, A., Stamou, A., Panagoutsos, S., Manolopoulos, V. G., Tsetsos, F., ... & Liakopoulos, V. (2021). Association of rs11780592 polymorphism in the human soluble epoxide hydrolase gene (EPHX2) with oxidized LDL and mortality in patients with diabetic chronic kidney disease. Oxidative medicine and cellular longevity, 2021.

Li, X., & Wu, X. (2021). Soluble epoxide hydrolase (Ephx2) silencing attenuates the hydrogen peroxide-induced oxidative damage in IEC-6 cells. Archives of Medical Science: AMS, 17(4), 1075.

Khadir, A., Kavalakatt, S., Madhu, D., Cherian, P., Al-Mulla, F., Abubaker, J., & Tiss, A. (2020). Soluble epoxide hydrolase 2 expression is elevated in obese humans and decreased by physical activity. International journal of molecular sciences, 21(6), 2056.

Demirdöğen, B. C., Miçooğulları, Y., Özçelik, A. T., & Adalı, O. (2020). Missense genetic polymorphisms of microsomal (EPHX1) and soluble epoxide hydrolase (EPHX2) and their relation to the risk of large artery atherosclerotic ischemic stroke in a Turkish population. Neuropsychiatric disease and treatment, 16, 3251.

Ma, L., Zhao, H., Yu, M., Wen, Y., Zhao, T., Yan, M., ... & Niu, W. (2020). Association of epoxide hydrolase 2 gene Arg287Gln with the risk for primary hypertension in Chinese. International journal of hypertension, 2020.

Sun, H., Lee, P., Yan, C., Gao, N., Wang, J., Fan, X., & Yu, F. S. (2018). Inhibition of soluble epoxide hydrolase 2 ameliorates diabetic keratopathy and impaired wound healing in mouse corneas. Diabetes, 67(6), 1162-1172.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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