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Mouse Anti-EPRS Recombinant Antibody (F-3) (CBMAB-P0314-YC)

Provided herein is a Mouse monoclonal antibody against Human Glutamyl-Prolyl-TRNA Synthetase. The antibody can be used for immunoassay techniques, such as WB, IP, IF, ELISA.
See all EPRS antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
F-3
Antibody Isotype
IgG
Application
WB, IP, IF, ELISA

Basic Information

Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Glutamyl-Prolyl-TRNA Synthetase
Introduction
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. Alternative splicing has been observed for this gene, but the full-length nature and biological validity of the variant have not been determined.
Entrez Gene ID
2058
UniProt ID
P07814
Alternative Names
EARS; GLUPRORS; PARS; PIG32; QARS; QPRS
Research Area
Multifunctional protein which is primarily part of the aminoacyl-tRNA synthetase multienzyme complex, also know as multisynthetase complex, that catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA (PubMed:1756734, PubMed:24100331, PubMed:23263184).

The phosphorylation of EPRS1, induced by interferon-gamma, dissociates the protein from the aminoacyl-tRNA synthetase multienzyme complex and recruits it to the GAIT complex that binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin), suppressing their translation. Interferon-gamma can therefore redirect, in specific cells, the EPRS1 function from protein synthesis to translation inhibition (PubMed:15479637, PubMed:23071094).

Also functions as an effector of the mTORC1 signaling pathway by promoting, through SLC27A1, the uptake of long-chain fatty acid by adipocytes. Thereby, it also plays a role in fat metabolism and more indirectly influences lifespan (PubMed:28178239).
Biological Process
Cellular response to insulin stimulus Source: UniProtKB
Cellular response to interferon-gamma Source: UniProtKB
Glutamyl-tRNA aminoacylation Source: InterPro
Negative regulation of translation Source: UniProtKB
Prolyl-tRNA aminoacylation Source: UniProtKB
Protein-containing complex assembly Source: ProtInc
Regulation of long-chain fatty acid import into cell Source: UniProtKB
tRNA aminoacylation for protein translation Source: Reactome
Cellular Location
Cytosol; Membrane. Translocates from cytosol to membranes upon phosphorylation at Ser-999.
Involvement in disease
Leukodystrophy, hypomyelinating, 15 (HLD15):
An autosomal recessive disorder characterized by hypomyelinating leukodystrophy with thinning of the corpus callosum. Clinical features include motor and cognitive impairment appearing in the first or second decade of life, dystonia, ataxia, spasticity, and dysphagia. Most patients develop severe optic atrophy, and some have hearing loss.
PTM
Phosphorylated at Ser-886 by CDK5 (PubMed:19647514, PubMed:21220307). Phosphorylated at Ser-999 by RPS6KB1; triggers EPRS1 release from the aminoacyl-tRNA synthetase multienzyme complex (PubMed:19647514, PubMed:21220307, PubMed:28178239). In monocytes, the IFN-gamma-induced sequential phosphorylation at Ser-886 and Ser-999 releases EPRS1 from the aminoacyl-tRNA synthetase multienzyme complex, allowing its association with the GAIT complex. Phosphorylation at Ser-999 is specifically required for the RPL13A-mediated interaction of the GAIT complex with eIF4G (PubMed:19647514, PubMed:21220307). Phosphorylation at Ser-999 by RPS6KB1, is also induced by insulin through activation of the mTORC1 signaling pathway and promotes the interaction of EPRS1 with SLC27A1 (PubMed:28178239).

Jin, D. (2021). Characterizing the role of the bifunctional glutamyl-prolyl-tRNA synthetase in human diseases (Doctoral dissertation, The Ohio State University).

Wu, J., Subbaiah, K. C. V., Xie, L. H., Jiang, F., Khor, E. S., Mickelsen, D., ... & Yao, P. (2020). Glutamyl-prolyl-tRNA synthetase regulates proline-rich pro-fibrotic protein synthesis during cardiac fibrosis. Circulation research, 127(6), 827-846.

Lee, C. H., Kim, D. W., Cho, M., Kim, J. M., Lee, J. H., Park, M. Y., ... & Park, J. S. (2020). Glutamyl-Prolyl-tRNA-Synthetase as a Novel Biomarker for Idiopathic Pulmonary Fibrosis. In A63. MEDIATORS OF LUNG FIBROSIS (pp. A2275-A2275). American Thoracic Society.

Song, D. G., Kim, D., Jung, J. W., Nam, S. H., Kim, J. E., Kim, H. J., ... & Lee, J. W. (2019). Glutamyl‐prolyl‐tRNA synthetase induces fibrotic extracellular matrix via both transcriptional and translational mechanisms. The FASEB Journal, 33(3), 4341-4354.

Eswarappa, S. M., Potdar, A. A., Sahoo, S., Sankar, S., & Fox, P. L. (2018). Metabolic origin of the fused aminoacyl-tRNA synthetase, glutamyl-prolyl-tRNA synthetase. Journal of Biological Chemistry, 293(49), 19148-19156.

Song, D. G., Kim, D., Jung, J. W., Nam, S. H., Kim, J. E., Kim, H. J., ... & Lee, J. W. (2018). Glutamyl-prolyl-tRNA synthetase regulates epithelial expression of mesenchymal markers and extracellular matrix proteins: implications for idiopathic pulmonary fibrosis. Frontiers in pharmacology, 9, 1337.

Schwarz, M. A., Lee, D. D., & Bartlett, S. (2018). Aminoacyl tRNA synthetase complex interacting multifunctional protein 1 simultaneously binds Glutamyl-Prolyl-tRNA synthetase and scaffold protein aminoacyl tRNA synthetase complex interacting multifunctional protein 3 of the multi-tRNA synthetase complex. The international journal of biochemistry & cell biology, 99, 197-202.

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For research use only. Not intended for any clinical use.

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