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Mouse Anti-ERF Recombinant Antibody (1E5) (CBMAB-A2699-LY)

The product is antibody recognizes ERF. The antibody 1E5 immunoassay techniques such as: WB, ELISA.
See all ERF antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1E5
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
ERF (AAH22231, 181 a.a. ~ 290 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Ets2 repressor factor
Introduction
Members of the ETS family of transcription factors, such as ERF, regulate cell proliferation and differentiation. They share a highly conserved DNA-binding domain, the ETS domain, that recognizes the sequence GGAA/T (de Castro et al., 1997 [PubMed 9192842]). For further information on ETS transcription factors, see ETS1 (MIM 164720).[supplied by OMIM
Entrez Gene ID
2077
UniProt ID
P50548
Alternative Names
PE-2; PE2
Research Area
Potent transcriptional repressor that binds to the H1 element of the Ets2 promoter. May regulate other genes involved in cellular proliferation. Required for extraembryonic ectoderm differentiation, ectoplacental cone cavity closure, and chorioallantoic attachment (By similarity).

May be important for regulating trophoblast stem cell differentiation (By similarity).
Biological Process
Cell differentiation Source: GO_Central
Negative regulation of transcription by RNA polymerase II Source: GO_Central
Regulation of transcription by RNA polymerase II Source: GO_Central
Cellular Location
Nucleus
Involvement in disease
Craniosynostosis 4 (CRS4):
A primary abnormality of skull growth involving premature fusion of one or more cranial sutures. The growth velocity of the skull often cannot match that of the developing brain resulting in an abnormal head shape and, in some cases, increased intracranial pressure, which must be treated promptly to avoid permanent neurodevelopmental disability.
Chitayat syndrome (CHYTS):
An autosomal dominant syndrome characterized by hyperphalangism, partial syndactyly, bilateral accessory phalanx resulting in shortened index fingers, hallux valgus, brachydactyly, facial anomalies, diffuse bronchomalacia, and respiratory distress at birth and in infancy.
PTM
Phosphorylated by multiple kinases including MAPK1/ERK2 at THR-526. Phosphorylation regulates the activity of ERF.

Tsiomita, S., Liveri, E. M., Vardaka, P., Vogiatzi, A., Skiadaresis, A., Saridis, G., ... & Thyphronitis, G. (2022). ETS2 repressor factor (ERF) is involved in T lymphocyte maturation acting as regulator of thymocyte lineage commitment. Journal of Leukocyte Biology.

Cannon-Albright, L. A., Teerlink, C. C., Stevens, J., Huang, F. W., Sipeky, C., Schleutker, J., ... & Trump, D. L. (2021). A Rare Variant in ERF (rs144812092) Predisposes to Prostate and Bladder Cancers in an Extended Pedigree. Cancers, 13(10), 2399.

Bao, X., Zhang, X., Wang, L., Wang, Z., Huang, J., Zhang, Q., ... & Xu, X. (2021). Epigenetic inactivation of ERF reactivates γ-globin expression in β-thalassemia. The American Journal of Human Genetics, 108(4), 709-721.

Yamada, M., Funato, M., Kondo, G., Suzuki, H., Uehara, T., Takenouchi, T., ... & Kosaki, K. (2021). Noonan syndrome‐like phenotype in a patient with heterozygous ERF truncating variant. Congenital Anomalies, 61(6), 226-230.

Vogiatzi, A., Baltsavia, I., Dialynas, E., Theodorou, V., Zhou, Y., Deligianni, E., ... & Mavrothalassitis, G. (2021). Erf affects commitment and differentiation of osteoprogenitor cells in cranial sutures via the retinoic acid pathway. Molecular and Cellular Biology, 41(8), e00149-21.

Yin, X., Zheng, X., Liu, M., Wang, D., Sun, H., Qiu, Y., ... & Shi, B. (2020). Exosomal miR‐663b targets Ets2‐repressor factor to promote proliferation and the epithelial–mesenchymal transition of bladder cancer cells. Cell Biology International, 44(4), 958-965.

Caro‐Contreras, A., Alcántara‐Ortigoza, M. A., Ahumada‐Pérez, J. F., & González‐del Angel, A. (2019). Molecular analysis provides further evidence that Chitayat syndrome is caused by the recurrent p.(Tyr89Cys) pathogenic variant in the ERF gene. American Journal of Medical Genetics Part A, 179(1), 118-122.

Glass, G. E., O'Hara, J., Canham, N., Cilliers, D., Dunaway, D., Fenwick, A. L., ... & Wilson, L. C. (2019). ERF‐related craniosynostosis: The phenotypic and developmental profile of a new craniosynostosis syndrome. American Journal of Medical Genetics Part A, 179(4), 615-627.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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