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Mouse Anti-ESR2 Monoclonal Antibody (14C8) (CBMAB-1215-YC)

Provided herein is a mouse monoclonal antibody against Human ESR2. The antibody, clone 14C8, can be used for immunoassay techniques, such as ChIP, FC, ICC, IF, IHC-Fr, IHC-P and WB.
See all ESR2 antibodies
Published Data
Fig.1 MCF-7 cells were transfected with control siRNA or siRNA targeting ERα (siERα) or ERβ (siERβ) for a total of 48 h.
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Fig.2 Immunohistochemical staining for Erβ.
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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Monkey, Baboon
Clone
14C8
Antibody Isotype
IgG2b, κ
Application
ChIP, FC, ICC, IF, IHC-Fr, IHC-P, WB

Basic Information

Immunogen
Recombinant 6-His fusion protein encoding aa 1-153 of human ER-beta expressed in E. coli.
Specificity
Human, Mouse, Monkey, Baboon
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
>95%, as determined by SDS-PAGE analysis
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Estrogen Receptor 2
Introduction
ESR2 is a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. ESR2 is localized to the nucleus, cytoplasm, and mitochondria. ESR2 forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription.
Entrez Gene ID
Human2100
Mouse13983
Monkey574110
Baboon100303418
UniProt ID
HumanQ92731
MouseO08537
MonkeyQ9TTE5
Alternative Names
Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA
Research Area
Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560).

Isoform 2:
Lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability.
Biological Process
Cell-cell signaling Source: ProtInc
Cellular response to estradiol stimulus Source: CAFA
Intracellular estrogen receptor signaling pathway Source: CAFA
Intracellular steroid hormone receptor signaling pathway Source: GO_Central
Negative regulation of cell growth Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Positive regulation of DNA-binding transcription factor activity Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: CAFA
Regulation of transcription, DNA-templated Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Signal transduction Source: ProtInc
Cellular Location
Nucleus
Involvement in disease
Ovarian dysgenesis 8 (ODG8):
A form of ovarian dysgenesis, a disorder characterized by lack of spontaneous pubertal development, primary amenorrhea, uterine hypoplasia, and hypergonadotropic hypogonadism as a result of streak gonads.
PTM
Phosphorylation at Ser-87 and Ser-105 recruits NCOA1.

Dalal, H., Dahlgren, M., Gladchuk, S., Brueffer, C., Gruvberger-Saal, S. K., & Saal, L. H. (2022). Clinical associations of ESR2 (estrogen receptor beta) expression across thousands of primary breast tumors. Scientific reports, 12(1), 1-12.

He, J., Yan, J. J., Zha, X., Ding, X. J., Zhang, Y. L., Lu, Z., & Xu, X. H. (2022). Sexually dimorphic effects of estrogen receptor 2 deletion in the dorsal raphe nucleus on emotional behaviors. Journal of Neuroendocrinology, e13195.

Asiamah, C. A., Liu, Y., Ye, R., Pan, Y., Lu, L. L., Zou, K., ... & Su, Y. (2022). Polymorphism analysis and expression profile of the estrogen receptor 2 gene in Leizhou black duck. Poultry Science, 101(3), 101630.

Gregorio, K. C. R., Laurindo, C. P., & Machado, U. F. (2021). Estrogen and glycemic homeostasis: the fundamental role of nuclear estrogen receptors ESR1/ESR2 in glucose transporter GLUT4 regulation. Cells, 10(1), 99.

Ulhaq, Z. S., & Garcia, C. P. (2021). Estrogen receptor beta (ESR2) gene polymorphism and susceptibility to dementia. Acta Neurologica Belgica, 121(5), 1281-1293.

Chmielewska, M., Janusz, P., Andrusiewicz, M., Kotwicki, T., & Kotwicka, M. (2020). Methylation of estrogen receptor 2 (ESR2) in deep paravertebral muscles and its association with idiopathic scoliosis. Scientific reports, 10(1), 1-14.

Bahia, W., Soltani, I., Haddad, A., Soua, A., Radhouani, A., Mahdhi, A., & Ferchichi, S. (2020). Association of genetic variants in Estrogen receptor (ESR) 1 and ESR2 with susceptibility to recurrent pregnancy loss in Tunisian women: A case control study. Gene, 736, 144406.

Lang-Muritano, M., Sproll, P., Wyss, S., Kolly, A., Hürlimann, R., Konrad, D., & Biason-Lauber, A. (2018). Early-onset complete ovarian failure and lack of puberty in a woman with mutated estrogen receptor β (ESR2). The Journal of Clinical Endocrinology & Metabolism, 103(10), 3748-3756.

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For research use only. Not intended for any clinical use.

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