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Mouse Anti-ETF1 (AA 338-437) Recombinant Antibody (CBFYE-1322) (CBMAB-E1930-FY)

This product is mouse antibody that recognizes ETF1. The antibody CBFYE-1322 can be used for immunoassay techniques such as: ELISA, WB.
See all ETF1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYE-1322
Antibody Isotype
IgG2b, κ
Application
ELISA, WB

Basic Information

Immunogen
ETF1 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Immunogen sequence: TEEEKILYLT PEQEKDKSHF TDKETGQEHE LIESMPLLEW FANNYKKFGA TLEIVTDKSQ EGSQFVKGFG GIGGILRYRV DFQGMEYQGG DDEFFDLDDY
Specificity
Human
Antibody Isotype
IgG2b, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 338-437

Target

Full Name
eukaryotic translation termination factor 1
Introduction
This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X.
Entrez Gene ID
2107
UniProt ID
P62495
Alternative Names
Eukaryotic Translation Termination Factor 1; Sup45 (Yeast Omnipotent Suppressor 45) Homolog-Like 1; Polypeptide Chain Release Factor 1; Protein Cl1; SUP45L1; TB3-1; ERF1
Research Area
Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA (PubMed:7990965, PubMed:24486019).

Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes (PubMed:30682371).
Biological Process
Cytoplasmic translational termination Source: GO_Central
Nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: UniProtKB-KW
Protein methylation Source: MGI
Regulation of translational termination Source: UniProtKB
Translational termination Source: MGI
Cellular Location
Cytoplasm
PTM
Methylated at Gln-185 by N6AMT1.
Hydroxylation at Lys-63 by JMJD4 promotes its translational termination efficiency.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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