Mouse Anti-ETF1 Recombinant Antibody (CBFYE-1322) (CBMAB-E1930-FY)

Name: Mouse Anti-ETF1 Recombinant Antibody (CBFYE-1322)
SKU: CBMAB-E1930-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-1322

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 1-300 mapping at the N-terminus of human Erf1.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Dog, Cattle, Pig

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Epitope

AA 338-437

More Infomation

Target

Full Name

eukaryotic translation termination factor 1

Introduction

This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X.

Entrez Gene ID

Human	2107
Mouse	225363
Rat	307503
Dog	608717
Monkey	714449

UniProt ID

Human	P62495
Mouse	Q8BWY3
Rat	Q5U2Q7
Dog	E2RBM3
Monkey	A0A2K5WYH8

Alternative Names

Eukaryotic Translation Termination Factor 1; Sup45 (Yeast Omnipotent Suppressor 45) Homolog-Like 1; Polypeptide Chain Release Factor 1; Protein Cl1; SUP45L1; TB3-1; ERF1

Research Area

Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA (PubMed:7990965, PubMed:24486019).

Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes (PubMed:30682371).

Biological Process

Cytoplasmic translational termination Source: GO_Central
Nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: UniProtKB-KW
Protein methylation Source: MGI
Regulation of translational termination Source: UniProtKB
Translational termination Source: MGI

Cellular Location

Cytoplasm

PTM

Methylated at Gln-185 by N6AMT1.
Hydroxylation at Lys-63 by JMJD4 promotes its translational termination efficiency.

Hu, A., Hong, F., Li, D., Xie, Q., Chen, K., Zhu, L., & He, H. (2022). KDM3B-ETF1 fusion gene downregulates LMO2 via the WNT/β-catenin signaling pathway, promoting metastasis of invasive ductal carcinoma. Cancer Gene Therapy, 29(2), 215-224.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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