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Mouse Anti-ETF1 Recombinant Antibody (2H4) (CBMAB-A2720-LY)

The product is antibody recognizes ETF1. The antibody 2H4 immunoassay techniques such as: WB, ELISA.
See all ETF1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2H4
Antibody Isotype
IgG2b, κ
Application
WB, ELISA

Basic Information

Immunogen
ETF1 (NP_004721.1, 338 a.a. ~ 437 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
eukaryotic translation termination factor 1
Introduction
Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA; however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).[supplied by OMIM
Entrez Gene ID
2107
UniProt ID
P62495
Alternative Names
D5S1995; ERF; ERF1; MGC111066; RF1; SUP45L1; TB3-1
Research Area
Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA (PubMed:7990965, PubMed:24486019).

Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes (PubMed:30682371).
Biological Process
Cytoplasmic translational termination Source: GO_Central
Nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: UniProtKB-KW
Protein methylation Source: MGI
Regulation of translational termination Source: UniProtKB
Translational termination Source: MGI
Cellular Location
Cytoplasm
PTM
Methylated at Gln-185 by N6AMT1.
Hydroxylation at Lys-63 by JMJD4 promotes its translational termination efficiency.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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