Mouse Anti-ETV4 Recombinant Antibody (1536) (V2LY-0825-LY875)

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Basic Information

Host Animal
Mouse
Clone
1536
Application
WB, ELISA
Immunogen
Full-length recombinant ETV3.
Host Species
Mouse
Specificity
Human, Mouse
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal Antibody

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
None
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
ETS Variant 4
Entrez Gene ID
Human2118
Mouse18612
UniProt ID
HumanP43268
MouseP28322
Research Area
Transcriptional activator (PubMed:19307308, PubMed:31552090).

May play a role in keratinocyte differentiation (PubMed:31552090).

(Microbial infection) Binds to the enhancer of the adenovirus E1A gene and acts as a transcriptional activator; the core-binding sequence is 5'-[AC]GGA[AT]GT-3'.
Biological Process
Cell differentiation Source: GO_Central
Positive regulation of keratinocyte differentiation Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Regulation of transcription by RNA polymerase II Source: GO_Central
Cellular Location
Nucleus
PTM
Sumoylated; enhanced upon ERK/MAP kinase pathway activation, it positively regulates the transcriptional activator capacity. Sumoylation at Lys-96 probably requires phosphorylation at Ser-101. Transiently polysumoylated and desumoylated by SENP1. Sumoylation is a prerequisite to polyubiquitination which in turn increases proteasomal-mediated degradation. Probably polyubiquitinated by RNF4 and deubiquitinated by USP2.

Wang, J., Sun, C., Li, J., Jiang, H., Qiu, Y., & Gong, M. (2022). Knockdown of ETV4 promotes autophagy‑dependent apoptosis in GBM cells by reducing the transcriptional activation of EMP1. Oncology Letters, 23(2), 1-10.

IV, A. N., & Nair, A. S. (2022). Bioinformatics screening of ETV4 transcription factor oncogenes and identifying small‐molecular anticancer drugs. Chemical Biology & Drug Design, 99(2), 277-285.

Wang, L., Zhang, Y., Yang, J., Liu, L., Yao, B., Tian, Z., & He, J. (2021). The Knockdown of ETV4 Inhibits the Papillary Thyroid Cancer Development by Promoting Ferroptosis Upon SLC7A11 Downregulation. DNA and Cell Biology, 40(9), 1211-1221.

Yao, D., Bao, Z., Qian, X., Yang, Y., & Mao, Z. (2021). ETV4 transcriptionally activates HES1 and promotes Stat3 phosphorylation to promote malignant behaviors of colon adenocarcinoma. Cell Biology International, 45(10), 2129-2139.

Yatagai, Y., Oshima, H., Sakamoto, T., Shigemasa, R., Kitazawa, H., Hyodo, K., ... & Hizawa, N. (2021). Expression quantitative trait loci for ETV4 and MEOX1 are associated with adult asthma in Japanese populations. Scientific reports, 11(1), 1-9.

Wang, B., Cai, Y., Li, X., Kong, Y., Fu, H., & Zhou, J. (2021). ETV4 mediated lncRNA C2CD4D-AS1 overexpression contributes to the malignant phenotype of lung adenocarcinoma cells via miR-3681-3p/NEK2 axis. Cell Cycle, 20(24), 2607-2618.

Wang, Y., Ding, X., Liu, B., Li, M., Chang, Y., Shen, H., ... & Li, Y. (2020). ETV4 overexpression promotes progression of non–small cell lung cancer by upregulating PXN and MMP1 transcriptionally. Molecular Carcinogenesis, 59(1), 73-86.

Deshmukh, S. K., Singh, A. P., & Singh, S. (2018). ETV4: an emerging target in pancreatic cancer. Oncoscience, 5(9-10), 260.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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