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Mouse Anti-FBXL7 Recombinant Antibody (2G10) (CBMAB-A2877-LY)

The product is antibody recognizes FBXL7. The antibody 2G10 immunoassay techniques such as: WB, ELISA.
See all FBXL7 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2G10
Antibody Isotype
IgG2b, κ
Application
WB, ELISA

Basic Information

Immunogen
FBXL7 (NP_036436, 392 a.a. ~ 489 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
F-box and leucine-rich repeat protein 7
Introduction
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains several tandem leucine-rich repeats. [provided by RefSeq]
Entrez Gene ID
23194
UniProt ID
Q9UJT9
Alternative Names
FBL6; FBL7
Research Area
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex (PubMed:25778398).

During mitosis, it mediates the ubiquitination and subsequent proteasomal degradation of AURKA, causing mitotic arrest (By similarity).

It also regulates mitochondrial function by mediating the ubiquitination and proteasomal degradation of the apoptosis inhibitor BIRC5 (PubMed:25778398, PubMed:28218735).
Biological Process
Cell division Source: UniProtKB-KW
G2/M transition of mitotic cell cycle Source: UniProtKB
Mitotic cell cycle Source: UniProtKB
Protein polyubiquitination Source: UniProtKB
Protein ubiquitination Source: UniProtKB
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
Ubiquitin-dependent protein catabolic process Source: UniProtKB
Cellular Location
Centrosome. Localizes to the centrosome during spindle formation.

Wang, Y., Shen, X., Gong, L., Zhao, Y., & Xiong, X. (2022). Functional characterization of FBXL7 as a novel player in human cancers. Cell Death Discovery, 8(1), 1-8.

Camuzi, D., Buexm, L. A., Lourenço, S. D. Q. C., Grazziotin, R., Guaraldi, S., Valverde, P., ... & Soares-Lima, S. C. (2022). FBXL7 body hypomethylation is frequent in tumors from the digestive and respiratory tracts and is associated with risk-factor exposure. International Journal of Molecular Sciences, 23(14), 7801.

Ni, J., Shen, L., Xu, L., Jin, Q., & Wang, G. (2021). 7-Ethoxyrosmanol alleviates hyperglycemia-induced vascular endothelial dysfunction by regulating FBXL7 expression. Journal of Bioenergetics and Biomembranes, 53(5), 553-560.

Moro, L., Simoneschi, D., Kurz, E., Arbini, A. A., Jang, S., Guaragnella, N., ... & Pagano, M. (2020). Epigenetic silencing of the ubiquitin ligase subunit FBXL7 impairs c-SRC degradation and promotes epithelial-to-mesenchymal transition and metastasis. Nature cell biology, 22(9), 1130-1142.

Boone, P. M., Paterson, S., Mohajeri, K., Zhu, W., Genetti, C. A., Tai, D. J., ... & Rodan, L. H. (2020). Biallelic mutation of FBXL7 suggests a novel form of Hennekam syndrome. American Journal of Medical Genetics Part A, 182(1), 189-194.

Kong, S., Fang, Y., Wang, B., Cao, Y., He, R., & Zhao, Z. (2020). miR‐152‐5p suppresses glioma progression and tumorigenesis and potentiates temozolomide sensitivity by targeting FBXL7. Journal of Cellular and Molecular Medicine, 24(8), 4569-4579.

Moro, L., & Pagano, M. (2020). Epigenetic suppression of FBXL7 promotes metastasis. Molecular & Cellular Oncology, 7(6), 1833698.

Chiu, H. W., Chang, J. S., Lin, H. Y., Lee, H. H., Kuei, C. H., Lin, C. H., ... & Lin, Y. F. (2018). FBXL7 upregulation predicts a poor prognosis and associates with a possible mechanism for paclitaxel resistance in ovarian cancer. Journal of clinical medicine, 7(10), 330.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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